Claudins regulate the intestinal barrier in response to immune mediators

Kinugasa, Tetsushi; Sakaguchi, Takanori; Gu, Xuibin; Reinecker, Hans–Christian

doi:10.1016/s0016-5085(00)70351-9

Cited by 403 publications

(279 citation statements)

References 41 publications

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“…Thus, claudin-2 expression in MDCKII cells is regulated through at least two independent pathways, involving RhoA and JNK, respectively. Previous studies have indicated an involvement of the ERK pathway in the regulation of claudin-2 expression (Kinugasa et al, 2000;Lan et al, 2004;Singh and Harris, 2004;Yamamoto et al, 2004;Murata et al, 2005). However, treatment of only MDCKI, but not MDCKII cells, with the ERK inhibitor U0126 up-regulates claudin-2, suggesting that the ERK pathway is not implicated in the up-regulation of claudin-2 expression in MDCKII cells (Lipschutz et al, 2005).…”

Section: Discussionmentioning

confidence: 91%

“…The MAPK/extracellular signal-regulated kinase (ERK) kinase pathway has been implicated in the control of claudin-2 expression (Kinugasa et al, 2000;Lan et al, 2004;Singh and Harris, 2004;Yamamoto et al, 2004;Lipschutz et al, 2005;Murata et al, 2005). To investigate the role of this and other signaling pathways in the up-regulation of claudin-2 and ZO-3 in cingulin KD clones, cingulin-depleted cells were treated with either the MAPK/ERK inhibitor U0126, the p38 kinase inhibitor SB202190, or the JNK inhibitor SP600125 ( Figure 3A).…”

Section: Jnk Regulate Claudin-2 Expression Independently Of Cingulin mentioning

confidence: 99%

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Cingulin Regulates Claudin-2 Expression and Cell Proliferation through the Small GTPase RhoA

Guillemot

Citi

2006

MBoC

138

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In mouse embryoid bodies, mutation of the tight junction protein cingulin results in changes in gene expression. Here, we studied the function of cingulin using a gene silencing approach in Madin-Darby canine kidney (MDCK) cells. Cingulin-depleted cells show higher protein and mRNA levels of claudin-2 and ZO-3, increased RhoA activity, activation of G 1 /S phase transition, and increased cell density. The effects of cingulin depletion on claudin-2 expression, cell proliferation, and density are reversed by coexpression of either a dominant-negative form of RhoA (RhoAN19) or the Rho-inhibiting enzyme C3 transferase. However, the increase in ZO-3 protein and mRNA levels is not reversed by inhibition of either RhoA, p38, extracellular signal-regulated kinase (ERK), or c-Jun NH 2 -terminal kinase (JNK), suggesting that cingulin modulates ZO-3 expression by a different mechanism. JNK is implicated in the regulation of claudin-2 levels independently of cingulin depletion and RhoA activity, indicating distinct roles of RhoA-and JNK-dependent pathways in the control of claudin-2 expression. Finally, cingulin depletion does not significantly alter the barrier function of monolayers and the overall molecular organization of tight junctions. These results provide novel insights about the mechanisms of cingulin function and the signaling pathways controlling claudin-2 expression in MDCK cells.

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Section: Discussionmentioning

confidence: 91%

Section: Jnk Regulate Claudin-2 Expression Independently Of Cingulin mentioning

confidence: 99%

Cingulin Regulates Claudin-2 Expression and Cell Proliferation through the Small GTPase RhoA

Guillemot

Citi

2006

MBoC

138

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“…In contrast, the diminished levels of IL-17 observed in Treg cells depleted mice may have a deleterious effect since this cytokine has been demonstrated to play a protective role at the intestinal mucosa during peroral T. gondii infection due to its capacity to recruit neutrophils to the site of infection [35]. Besides, it has also been shown to play a role in the induction of tight junction formation of intestinal epithelial cells and thereby thought to regulate intestinal barrier function [36]. One could therefore speculate that the smaller amounts of IL-17 produced in the presence of higher levels of pro-inflammatory cytokines (e.g., IFN-g and IL-6), weakened the intestinal barrier and favoured an increase in parasite entry into the LP.…”

Section: Discussionmentioning

confidence: 99%

Partial depletion of CD4+CD25+Foxp3+ T regulatory cells significantly increases morbidity during acute phase Toxoplasma gondii infection in resistant BALB/c mice

Morampudi

Craeye

Moine

et al. 2011

Microbes and Infection

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CD4 þ CD25 þ Foxp3 þ T regulatory (Treg) cells, are known to regulate responses to infectious agents. Here we compared disease progression in BALB/c and C57BL/6(B6) mice infected perorally with Toxoplasma gondii for 7 days and examined the affect of partial depletion of Treg cells in these mice. BALB/c mice were seen to be resistant to peroral infection whereas B6 mice were susceptible in terms of mortality. Although the depletion of Treg cells before infection had no effect on the survival of B6 or BALB/c mice, it resulted in increased parasite burdens in BALB/c mice, especially in the lamina propria, but not in B6 mice. Pro-inflammatory cytokines were also increased in Treg cells depleted BALB/c mice as compared to B6 mice. In addition Treg cell depleted BALB/c mice displayed increased ileal histopathology compared to their non-treated counterparts. These findings provide evidence for the contribution of Treg cells, in the resistance of BALB/c mice against peroral T. gondii infection.

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“…Moreover, we also detected the production of IL-17 in the DSS + rIL-25 treated mice and DSS treated mice but without any significant difference. In spite of the fact that the role for IL-17 in IBD is unclear, IL-17 has been shown to protect mucosal barrier functions through induction of tight junction formation (29). The role for IL-17 was lately proven through neutralization of IL-17 that resulted in exacerbation of DSS-induced colitis (30).…”

Section: Discussionmentioning

confidence: 99%

Inhibitory Effect of Recombinant IL-25 on the Development of Dextran Sulfate Sodium-Induced Experimental Colitis in Mice

et al. 2008

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Claudins regulate the intestinal barrier in response to immune mediators

Cited by 403 publications

References 41 publications

Cingulin Regulates Claudin-2 Expression and Cell Proliferation through the Small GTPase RhoA

Cingulin Regulates Claudin-2 Expression and Cell Proliferation through the Small GTPase RhoA

Partial depletion of CD4+CD25+Foxp3+ T regulatory cells significantly increases morbidity during acute phase Toxoplasma gondii infection in resistant BALB/c mice

Inhibitory Effect of Recombinant IL-25 on the Development of Dextran Sulfate Sodium-Induced Experimental Colitis in Mice

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