Clarifying electron transfer and metagenomic analysis of microbial community in the methane production process with the addition of ferroferric oxide

Yin, Qidong; Yang, Shuo; Wang, Zhongzhong; Xing, Lei; Wu, Guangxue

doi:10.1016/j.cej.2017.09.160

Cited by 316 publications

(72 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Such properties allow DIET establishment among syntrophic partners and methanogens [48]. Enhanced sludge conductivity was proven to have a direct relation with electron transport efficiency, in AD broth, which reflects on CH 4 production [49]. Electrical aggregates are recently denoted as one of DIET occurrences [50].…”

Section: Conductive Material-based Broth Changementioning

confidence: 99%

Enhanced Anaerobic Digestion of Long Chain Fatty Acid by Adding Magnetite and Carbon Nanotubes

Mostafa

Song

et al. 2020

Microorganisms

View full text Add to dashboard Cite

This study investigated the impact of stimulating direct interspecies electron transfer (DIET), by supplementing nano-sized magnetite (nFe3O4, 0.5 g Fe/g VSS) and carbon nanotubes (CNT, 1 g/L), in anaerobic digestion of oleic acid (OA) at various concentrations (0.10–4.00 g chemical oxygen demand(COD)/L). Both supplementations could enhance CH4 production, and its beneficial impact increased with increased OA concentration. The biggest improvements of 114% and 165% compared to the control were achieved by nFe3O4 and CNT, respectively, at OA of 4 g COD/L. The enhancement can be attributed to the increased sludge conductivity: 7.1 ± 0.5 (control), 12.5 ± 0.8 (nFe3O4-added), and 15.7 ± 1.1 µS/cm (CNT-supplemented). Dissolved iron concentration, released from nFe3O4, seemed to have a negligible role in improving CH4 production. The excretion of electron shuttles, i.e., humic-like substances and protein-like substances, were found to be stimulated by supplementing nFe3O4 and CNT. Microbial diversity was found to be simplified under DIET-stimulating conditions, whereby five genera accounted for 88% of the total sequences in the control, while more than 82% were represented by only two genera (Methanotrix concilli and Methanosarcina flavescens) by supplementing nFe3O4 and CNT. In addition, the abudance of electro-active bacteria such as Syntrophomonas zehnderi was significantly increased from 17% to around 45%.

show abstract

Section: Conductive Material-based Broth Changementioning

confidence: 99%

Enhanced Anaerobic Digestion of Long Chain Fatty Acid by Adding Magnetite and Carbon Nanotubes

Mostafa

Song

et al. 2020

Microorganisms

View full text Add to dashboard Cite

show abstract

“…Until now, many conductive materials have been confirmed to be able to promote the performance of methanogenesis, such as increasing of the CH 4 production rate and chemical oxygen demand (COD) removal rate, and shortening of the lag phase of methanogenesis ( Zhao et al, 2015 ; Yin et al, 2017a ). Methanogenesis can be enhanced by ferroferric oxide (Fe 3 O 4 ) with the improvement of the extracellular electron transfer activity of the electron transport chain ( Yin et al, 2018 ). Nevertheless, how electron is transported from surfaces of microorganisms to conductive materials remains unknown.…”

Section: Introductionmentioning

confidence: 99%

Ferroferric Oxide Significantly Affected Production of Soluble Microbial Products and Extracellular Polymeric Substances in Anaerobic Methanogenesis Reactors

Yin

Echigo

et al. 2018

Front. Microbiol.

Self Cite

View full text Add to dashboard Cite

Conductive materials facilitate direct interspecies electron transfer between acidogens and methanogens during methane (CH4) production. Soluble microbial products (SMP) and extracellular polymeric substances (EPS) produced by microorganisms might act as the electron shuttle between microorganisms and conductive materials. In this study, effects of conductive ferroferric oxide (Fe3O4) on anaerobic treatment process and the production of SMP and EPS were investigated. The maximum CH4 production rate was enhanced by 23.3% with the dosage of Fe3O4. The concentrations of proteins, polysaccharides, and humic substances in tightly bound EPS (T-EPS) were promoted, suggesting that extracellular metabolisms were induced by conductive materials. Distribution of potential electron shuttles such as quinone-like substances, flavins, aromatic amino acids, and dipeptides in SMP and EPS phases were comprehensively investigated and these electron shuttles were significantly affected by Fe3O4. Dipeptides consisting of phenylalanine were widely detected in T-EPS of the Fe3O4 reactor, indicating a potential different extracellular electron exchange pattern with the addition of conductive materials.

show abstract

“…During acidification, soluble monomers are converted into short-chain fatty acids, such as acetic acid, which is the direct substrate for methanogens bacteria, as well as into propionic acid. To increase the production and the rate of conversion of monomers into acetic acid, different approaches can be considered, one of which is the use of substances that favor the bioconversion of carbohydrates, as for example cysteine [18], polycyclic aromatic hydrocarbons [19], and magnetite [20]. After the improvement of the first three stages, the methanogenesis stage is a key process to increase methane production.…”

Section: Introductionmentioning

confidence: 99%

An Incubation System to Enhance Biogas and Methane Production: A Case Study of an Existing Biogas Plant in Umbria, Italy

et al. 2019

View full text Add to dashboard Cite

The pre-incubation of digestate and recycling of microbes inside a continuously stirred tank reactor (CSTR) are effective ways to optimize the anaerobic digestion process and improve the performance of biogas and methane production, also in existing biogas plants. In this study, a digestate incubation system using a nutrient mix to boost the activity of microbes was coupled to a CSTR to boost biogas and methane production. This system has been tested both on a lab scale and on an industrial scale. On a pilot scale, the system achieved an increase of +16.47 v% in biogas production with respect to the conventional anaerobic digestion process, and an increase of +2 v% in methane content (from 65.94 v% to 67.84 v%). On an industrial scale, the use of this incubation reactor with a capacity of 1 m3 has led to an increase in methane yield of 12 v%. This system allows to maintain the syntrophic relationship between acid-producing bacteria and methanogens and contemporary push the development of methanogens. Moreover, it is an economic system to be integrated into an existing biogas plant given the small volume and the simplicity of the incubation reactor.

show abstract

Clarifying electron transfer and metagenomic analysis of microbial community in the methane production process with the addition of ferroferric oxide

Cited by 316 publications

References 41 publications

Enhanced Anaerobic Digestion of Long Chain Fatty Acid by Adding Magnetite and Carbon Nanotubes

Enhanced Anaerobic Digestion of Long Chain Fatty Acid by Adding Magnetite and Carbon Nanotubes

Ferroferric Oxide Significantly Affected Production of Soluble Microbial Products and Extracellular Polymeric Substances in Anaerobic Methanogenesis Reactors

An Incubation System to Enhance Biogas and Methane Production: A Case Study of an Existing Biogas Plant in Umbria, Italy

Contact Info

Product

Resources

About