Clarification of the C-35 Stereochemistries of Dinophysistoxin-1 and Dinophysistoxin-2 and Its Consequences for Binding to Protein Phosphatase

Larsen, Kristofer; Petersen, Dirk; Wilkins, Alistair L.; Samdal, Ingunn A.; Sandvik, Morten; Rundberget, Thomas; Goldstone, David C.; Arcus, Vickery L.; Hovgaard, Peter; Rise, Frode; Rehmann, Nils; Heß, Philipp; Miles, Christopher O.

doi:10.1021/tx700016m

Cited by 55 publications

(51 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The cells were rinsed on the sieve with fresh f/2 media, and kept wet to minimize breakage. The cells were washed into a 15-mL Falcon tube Tachibana and Scheuer, 1981;(2) Murata et al, 1982;(3) Larsen et al, 2007;(4) Yasumoto et al, 1985;(5) Marr et al, 1992 (6) Hu et al, 1995a;(7) Hu et al, 1995b;(8) Cruz et al, 2006;(9) Fernandez et al, 2003;(10) Suarez-Gomez et al, 2005;(11) Yasumoto et al, 1989;(12) Hu et al, 1992a;(13) Suarez-Gomez et al, 2001;(14) Miles et al, 2006;(15) Torgersen et al, 2008a;(16) Norte et al, 1994. using fresh f/2 media (13 mL), and the tube immersed in a water bath at 100 C for 5 min. The samples were frozen at À20 C, thawed at room temperature, and sonicated in a water bath (Fisher ultrasonic cleaner, Model FS30H) for 15 min before solid-phase extraction (SPE).…”

Section: Sample Preparation and Extraction Of Toxinsmentioning

confidence: 99%

Toxin profiles of five geographical isolates of Dinophysis spp. from North and South America

Fux

Smith

Tong

et al. 2011

Toxicon

View full text Add to dashboard Cite

a b s t r a c tMarine dinoflagellates of the genus Dinophysis can produce toxins of the okadaic acid (OA) and pectenotoxin (PTX) groups. These lipophilic toxins accumulate in filter-feeding shellfish and cause an illness in consumers called diarrhetic shellfish poisoning (DSP). In 2008, a bloom of Dinophysis led to the closure of shellfish harvesting areas along the Texas coast, one of the first DSP-related closures in the U.S. This event resulted in a broad study of toxin production in isolates of Dinophysis spp. from U.S. waters. In the present study, we compared toxin profiles in geographical isolates of Dinophysis collected in the U.S. (Eel Pond, Woods Hole MA; Martha's Vineyard, MA; and Port Aransas Bay, Texas), and in those from Canada (Blacks Harbour, Bay of Fundy) and Chile (Reloncavi Estuary), when cultured in the laboratory under the same conditions. For each isolate, the mitochondrial cox1 gene was sequenced to assist in species identification. Strains from the northeastern U.S. and Canada were all assigned to Dinophysis acuminata, while those from Chile and Texas were most likely within the D. acuminata complex whereas precise species designation could not be made with this marker. Toxins were detected in all Dinophysis isolates and each isolate had a different profile. Toxin profiles of isolates from Eel Pond, Martha's Vineyard, and Bay of Fundy were most similar, in that they all contained OA, DTX1, and PTX2. The Eel Pond isolate also contained OA-D8 and DTX1-D7, and low levels (unconfirmed structurally) of DTX1-D8 and DTX1-D9. D. acuminata from Martha's Vineyard produced DTX1-D7, along with OA, DTX1, and PTX2, as identified in both the cells and the culture medium. D. acuminata from the Bay of Fundy produced DTX1 and PTX2, as found in both cells and culture medium, while only trace amounts of OA were detected in the medium. The Dinophysis strain from Texas only produced OA, and the one from Chile only PTX2, as confirmed in both cells and culture medium.Published by Elsevier Ltd.

show abstract

Section: Sample Preparation and Extraction Of Toxinsmentioning

confidence: 99%

Toxin profiles of five geographical isolates of Dinophysis spp. from North and South America

Fux

Smith

Tong

et al. 2011

Toxicon

View full text Add to dashboard Cite

show abstract

“…Estrutura química da ficotoxina diarreica ácido okadaico (OA) e seus principais derivados dinophysistoxina 1 (DTX1), dinophysistoxina 2 (DTX2) e dinophysistoxina 3 (DTX3). Figura adaptada de Larsen et al (2007). Papel das Ficotoxinas Diarreicas na Biota Aquática e Aquicultura preferencialmente em vieiras.…”

Section: Toxinas Diarreicasunclassified

The Role of Diarroethic Phycotoxins in Aquatic Biota Andaquaculture

Ferreira

Pereira

2016

Oecol. Austr.

View full text Add to dashboard Cite

RESUMOO presente artigo contextualiza o papel das ficotoxinas diarreicas em três cenários: seu papel ecológico em relação à biota marinha, seus efeitos na aquicultura e, incluindo o homem na teia trófica marinha, seus efeitos deletérios na saúde pública. Primeiramente, ficotoxinas são definidas como produtos naturais marinhos, substâncias biologicamente ativas (metabólitos secundários), sendo as diarreicas sintetizadas por dinoflagelados dos gêneros Dinophysis e Prorocentrum. Esses organismos integram a comunidade planctônica marinha costeira, sendo algumas espécies de Prorocentrum bentônicas; portanto as toxinas apresentam implicações nas teias tróficas pelágicas e bentônicas costeiras. São ditas microalgas potencialmente tóxicas, pois a produção das ficotoxinas não é constante e varia intraespecificamente dependendo do contexto ecológico em que a microalga se encontre. Moluscos bivalvos (mexilhões, ostras e vieiras) são o segundo grupo mais cultivado na aquicultura marinha. São organismos bentônicos sésseis e alimentam-se principalmente de microalgas. Quando as microalgas potencialmente tóxicas estiverem expressando sua capacidade toxígena, os moluscos as bioacumularão, transferindo as ficotoxinas para os consumidores humanos. Dessa forma, surgem as Síndromes de Envenenamento por Moluscos, maior problema de saúde pública em nível mundial, associadas ao consumo de moluscos bivalvos contaminados por ficotoxinas. Essa revisão trata especificamente do Envenenamento Diarreico por Moluscos (EDM), intoxicação causada principalmente pela ficotoxina ácido okadáico, amplamente distribuída em águas temperadas às tropicais. Atualmente, a aquicultura corresponde a 47% do pescado produzido, com tendência a aumentar sua participação em virtude da estagnação na captura de pescado. Dessa forma, para garantir que o pescado produzido seja um alimento inócuo aos consumidores humanos, foi necessário desenvolver políticas públicas voltadas à sanidade do pescado. No cenário nacional, recentemente foi criado o Programa Nacional de Controle Higiênico Sanitário de Moluscos Bivalvos, ainda não implementado, em moldes de programas internacionais em vigor há décadas. A plena execução do programa é fundamental ao fomento seguro da aquicultura nacional. Palavras-chave: Dinophysis; envenenamento diarreico por moluscos; ficotoxinas; malacocultura; Prorocentrum. ABSTRACT -THE ROLE OF DIARROETHIC PHYCOTOXINS IN AQUATIC BIOTA ANDAQUA-CULTUREThis article analyzes the role of diarroethic phycotoxins in three scenarios: their ecological role in relation to marine biota, their effects on aquaculture -including the man in the marine trophic web -and its deleterious effects on public health. First, phycotoxins are defined as marine natural products. Biologically active (secondary metabolites) being the diarroethic ones-synthesized by dinoflagellates from the genus Dinophysis and Prorocentrum. These dinoflagellates comprise the coastal marine plankton community, but some species of toxic Prorocentrum are benthic. So the toxins have implications for ben...

show abstract

“…The chain length of the acyl moiety can range from C 14 to C 22 and may contain between 0 and 6 unsaturated carbon-carbon bonds. The most predominant fatty acid identified in the acylated derivatives is reported to be hexadecanoic (palmitic, C 16:0 ) acid (cited in Hallegraeff et al, 1995;Wright, 1995;EU/SANCO, 2001 Larsen et al, 2007).…”

Section: Chemical Characteristicsmentioning

confidence: 99%

Untitled

2008

EFSA Journal

View full text Add to dashboard Cite

Clarification of the C-35 Stereochemistries of Dinophysistoxin-1 and Dinophysistoxin-2 and Its Consequences for Binding to Protein Phosphatase

Cited by 55 publications

References 28 publications

Toxin profiles of five geographical isolates of Dinophysis spp. from North and South America

Toxin profiles of five geographical isolates of Dinophysis spp. from North and South America

The Role of Diarroethic Phycotoxins in Aquatic Biota Andaquaculture

Untitled

Contact Info

Product

Resources

About