CLAMP, a novel microtubule‐associated protein with EB‐type calponin homology

Dougherty, Gerard W.; Adler, Henry J.; Rzadzinska, Agnieszka K.; Gimona, Mario; Tomita, York; Lattig, M. Claudia; Merritt, Raymond C.; Kachar, Bechara

doi:10.1002/cm.20100

Cited by 3 publications

(3 citation statements)

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“…Contrary to studies of Xenopus epidermal cells, which are facilitated by the availability of markers used in immunofluorescence (26)(27)(28), mammalian cilia polarity is usually investigated by transmission EM (4,6,7,29,30), which is hardly compatible with tissue-wide polarity analysis. To circumvent this difficulty, we tested a variety of markers and found that phospho-β-catenin (P-βCat) (31)(32)(33), Chibby (29), FGFR1 Oncogene Partner (34), and Clamp (26,35) localized at the base of cilia, and when combined with γ-tubulin immunostaining, they clearly delineate cilia polarity. The P-βCat signal was adjacent to that of γ-tubulin; at the side opposite to the basal foot, a lateral extension of BBs pointing in the direction of the cilia beats effective stroke (Fig.…”

Section: Significancementioning

confidence: 99%

A dual role for planar cell polarity genes in ciliated cells

Boutin

Labedan²,

Dimidschstein

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

121

205

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In the nervous system, cilia dysfunction perturbs the circulation of the cerebrospinal fluid, thus affecting neurogenesis and brain homeostasis. A role for planar cell polarity (PCP) signaling in the orientation of cilia (rotational polarity) and ciliogenesis is established. However, whether and how PCP regulates cilia positioning in the apical domain (translational polarity) in radial progenitors and ependymal cells remain unclear. By analysis of a large panel of mutant mice, we show that two PCP signals are operating in ciliated cells. The first signal, controlled by cadherin, EGF-like, laminin G-like, seven-pass, G-type receptor (Celsr) 2, Celsr3, Frizzled3 (Fzd3) and Van Gogh like2 (Vangl2) organizes multicilia in individual cells (single-cell polarity), whereas the second signal, governed by Celsr1, Fzd3, and Vangl2, coordinates polarity between cells in both radial progenitors and ependymal cells (tissue polarity). Loss of either of these signals is associated with specific defects in the cytoskeleton. Our data reveal unreported functions of PCP and provide an integrated view of planar polarization of the brain ciliated cells.

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Section: Significancementioning

confidence: 99%

A dual role for planar cell polarity genes in ciliated cells

Boutin

Labedan²,

Dimidschstein

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

121

205

View full text Add to dashboard Cite

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“…One head contains Nuf2 and Ndc80, which each contain positively charged calponinhomology domains (CH) that facilitate binding to the negative microtubule surface (Wei et al 2005;Cheeseman et al 2006;Wei et al 2007;Ciferri et al 2008). CH domains have diverse functions and have been identified in other microtubule binding proteins (Hayashi and Ikura 2003;Dougherty et al 2005). An unstructured N-terminal tail on Ndc80 enhances the microtubule binding activity of the complex (Wei et al 2005;DeLuca et al 2006;Wei et al 2007;Ciferri et al 2008;Miller et al 2008;Alushin et al 2010), although it is not essential for yeast viability due to redundancy with Dam1 (Kemmler et al 2009;Demirel et al 2012;Lampert et al 2013).…”

Section: Kmnmentioning

confidence: 99%

The Composition, Functions, and Regulation of the Budding Yeast Kinetochore

2013

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The propagation of all organisms depends on the accurate and orderly segregation of chromosomes in mitosis and meiosis. Budding yeast has long served as an outstanding model organism to identify the components and underlying mechanisms that regulate chromosome segregation. This review focuses on the kinetochore, the macromolecular protein complex that assembles on centromeric chromatin and maintains persistent load-bearing attachments to the dynamic tips of spindle microtubules. The kinetochore also serves as a regulatory hub for the spindle checkpoint, ensuring that cell cycle progression is coupled to the achievement of proper microtubule-kinetochore attachments. Progress in understanding the composition and overall architecture of the kinetochore, as well as its properties in making and regulating microtubule attachments and the spindle checkpoint, is discussed. TABLE OF CONTENTS Abstract 817Introduction 818

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“…Spef1/CLAMP interacts with microtubules through a calponin homology domain and thus promotes microtubule bundling and microtubule stabilization (35). In T. brucei, a single Spef1 homolog (also known as TbCMF18) is present.…”

Section: Identification Of Bi-lobe-associated Proteins By Lc/ms-ms-mentioning

confidence: 99%

Biochemical Characterization of the Bi-lobe Reveals a Continuous Structural Network Linking the Bi-lobe to Other Single-copied Organelles in Trypanosoma brucei

Gheiratmand

Brasseur

Zhou

et al. 2013

Journal of Biological Chemistry

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Background:The bi-lobe is a structure critical to biogenesis and inheritance of several single-copied organelles in Trypanosoma brucei. Results: The bi-lobe was immunoisolated, and new bi-lobe associated proteins were characterized. Conclusion: A continuous structural network connected bi-lobe to other organelles in Trypanosoma brucei. Significance: Structural tethering may be a general strategy to ensure orderly and faithful inheritance of single-copied organelles in protists.

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CLAMP, a novel microtubule‐associated protein with EB‐type calponin homology

Cited by 3 publications

References 0 publications

A dual role for planar cell polarity genes in ciliated cells

A dual role for planar cell polarity genes in ciliated cells

The Composition, Functions, and Regulation of the Budding Yeast Kinetochore

Biochemical Characterization of the Bi-lobe Reveals a Continuous Structural Network Linking the Bi-lobe to Other Single-copied Organelles in Trypanosoma brucei

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