Citrus carotenoid isomerase gene characterization by complementation of the “Micro-Tom” tangerine mutant

Pinheiro, Thaísa Tessutti; Peres, Lázaro Eustáquio Pereira; Purgatto, Eduardo; Latado, Rodrigo Rocha; Maniero, Rodolfo A.; Martins, Mônica M.; Figueira, Antônio

doi:10.1007/s00299-019-02393-2

Cited by 10 publications

(10 citation statements)

References 49 publications

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“…), Ficus carica , S. baicalensis , and wolfberry [ 26 , 31 , 33 , 37 , 38 , 39 , 40 ]; carotenoid isomerase ( CrtISO ) in cabbage, citrus ( Citrus sinensis L Osbeck; cv. “Pera”), and wolfberry [ 33 , 41 , 42 ]; lycopene β-cyclase ( LCYB ) in Arabidopsis , citrus ( Citrus unshiu Marc., Citrus sinensis Osbeck, Citrus limon Burm.f. ), I. dentate , maize, papaya, persimmon, strawberry, sweet range, and wheat [ 26 , 29 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 ]; lycopene ε-cyclase ( LCYE ) in Arabidopsis , citrus ( Citrus unshiu Marc., Citrus sinensis Osbeck, Citrus limon Burm.f.…”

Section: Introductionmentioning

confidence: 99%

Molecular Characterization, Expression Analysis of Carotenoid, Xanthophyll, Apocarotenoid Pathway Genes, and Carotenoid and Xanthophyll Accumulation in Chelidonium majus L.

Sathasivam

Yeo

Park

et al. 2021

Plants

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Chelidonium majus L. is a perennial herbaceous plant that has various medicinal properties. However, the genomic information about its carotenoid biosynthesis pathway (CBP), xanthophyll biosynthesis pathway (XBP), and apocarotenoid biosynthesis pathway (ABP) genes were limited. Thus, the CBP, XBP, and ABP genes of C. majus were identified and analyzed. Among the 15 carotenoid pathway genes identified, 11 full and 4 partial open reading frames were determined. Phylogenetic analysis of these gene sequences showed higher similarity with higher plants. Through 3D structural analysis and multiple alignments, several distinct conserved motifs were identified, including dinucleotide binding motif, carotene binding motif, and aspartate or glutamate residues. Quantitative RT-PCR showed that CBP, XBP, and ABP genes were expressed in a tissue-specific manner; the highest expression levels were achieved in flowers, followed by those in leaves, roots, and stems. The HPLC analysis of the different organs showed the presence of eight different carotenoids. The highest total carotenoid content was found in leaves, followed by that in flowers, stems, and roots. This study provides information on the molecular mechanisms involved in CBP, XBP, and ABP genes, which might help optimize the carotenoid production in C. majus. The results could also be a basis of further studies on the molecular genetics and functional analysis of CBP, XBP, and ABP genes.

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Section: Introductionmentioning

confidence: 99%

Molecular Characterization, Expression Analysis of Carotenoid, Xanthophyll, Apocarotenoid Pathway Genes, and Carotenoid and Xanthophyll Accumulation in Chelidonium majus L.

Sathasivam

Yeo

Park

et al. 2021

Plants

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“…Despite being involved in the photo-inhibition of the PSII, suppression of VDE can induce, at the same time, an accumulation of fucoxanthin that functions as an efficient anti-oxidant in anoxia conditions ( Mikami and Hosokawa, 2013 ). The downregulation of carotenoid isomerase was demonstrated to induce an accumulation of zeta-carotene and cis -prolycopene in tomato fruits ( Isaacson et al, 2002 ; Pinheiro et al, 2019 ), both elevating and modifying carotenoid profiles toward more bioavailable forms compared to wild-type ( Cooperstone et al, 2015 ). Therefore, the ROS counteracting potential of PhAN4 HRC material may be related not only to anthocyanins but also to other accumulating anti-oxidant specialized metabolites.…”

Section: Discussionmentioning

confidence: 99%

Modifying Anthocyanins Biosynthesis in Tomato Hairy Roots: A Test Bed for Plant Resistance to Ionizing Radiation and Antioxidant Properties in Space

et al. 2022

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Gene expression manipulation of specific metabolic pathways can be used to obtain bioaccumulation of valuable molecules and desired quality traits in plants. A single-gene approach to impact different traits would be greatly desirable in agrospace applications, where several aspects of plant physiology can be affected, influencing growth. In this work, MicroTom hairy root cultures expressing a MYB-like transcription factor that regulates the biosynthesis of anthocyanins in Petunia hybrida (PhAN4), were considered as a testbed for bio-fortified tomato whole plants aimed at agrospace applications. Ectopic expression of PhAN4 promoted biosynthesis of anthocyanins, allowing to profile 5 major derivatives of delphinidin and petunidin together with pelargonidin and malvidin-based anthocyanins, unusual in tomato. Consistent with PhAN4 features, transcriptomic profiling indicated upregulation of genes correlated to anthocyanin biosynthesis. Interestingly, a transcriptome reprogramming oriented to positive regulation of cell response to biotic, abiotic, and redox stimuli was evidenced. PhAN4 hairy root cultures showed the significant capability to counteract reactive oxygen species (ROS) accumulation and protein misfolding upon high-dose gamma irradiation, which is among the most potent pro-oxidant stress that can be encountered in space. These results may have significance in the engineering of whole tomato plants that can benefit space agriculture.

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“…After determining that BoaCRTISO partial suppression can yellow Chinese kale leaves, we further explored the relationship among its phenotype, gene expression, and carotenoid contents. Prior work showed that the "Micro Tom" tomato was silenced using an inverted repeat of a fragment from the Citrus CRTISO that resulted in an overall decrease in transcript accumulation of all genes from the carotenoid biosynthetic pathway (Pinheiro et al, 2019), whereas downregulation of CRTISO expression in tomato (Isaacson et al, 2002) and cabbage (Su et al, 2015) can lead to upregulation of upstream gene expression and downregulation of downstream gene expression in the carotenoid biosynthetic pathway, causing the accumulation of pro-lycopene. In our study, we found that after BoaCRTISO expression was downregulated, almost all carotenoid biosynthetic gene expressions in Chinese kale were also downregulated; this finding is consistent with results reported on citrus (Pinheiro et al, 2019) but inconsistent with that known from tomato (Isaacson et al, 2002) and cabbage (Su et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

“…Several major carotenoids in plants appear in their trans form through the activity of isomerases, which are enzymes that function to convert molecules from one isomer to another. For example, carotenoid isomerase (CRTISO) is a key isomerase in the carotenoid biosynthetic pathway, which can catalyze prolycopene to lycopene (Pinheiro et al, 2019). In Arabidopsis (Park et al, 2002), tomato (Isaacson et al, 2002), and cabbage (Su et al, 2015), the loss of CRTISO function could cause the accumulation of pro-lycopene, which delays the greening of Arabidopsis, and changes the tomato color from red to orange and the cabbage color from yellow to orange.…”

Section: Introductionmentioning

confidence: 99%

Characterization of BoaCRTISO Reveals Its Role in Carotenoid Biosynthesis in Chinese Kale

et al. 2021

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Carotenoids are organic pigments that play an important role in both plant coloration and human health; they are a critical subject in molecular breeding due to growing demand for natural molecules in both food and medicine. In this study, we focus upon characterizing BoaCRTISO, the carotenoid isomerase gene before the branch of the carotenoid biosynthetic pathway, which is expressed in all organs and developmental stages of Chinese kale, and BoaCRTISO, which is located in the chloroplast. The expression of BoaCRTISO is induced by strong light, red and blue combined light, and gibberellic acid treatment, but it is suppressed by darkness and abscisic acid treatment. We obtained BoaCRTISO-silenced plants via virus-induced gene silencing technology, and the silence efficiencies ranged from 52 to 77%. The expressions of most carotenoid and chlorophyll biosynthetic genes in BoaCRTISO-silenced plants were downregulated, and the contents of carotenoids and chlorophyll were reduced. Meanwhile, BoaCRTISO-silenced plants exhibited phenotypes of yellowing leaves and inhibited growth. This functional characterization of BoaCRTISO provides insight for the biosynthesis and regulation of carotenoid in Chinese kale.

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Citrus carotenoid isomerase gene characterization by complementation of the “Micro-Tom” tangerine mutant

Cited by 10 publications

References 49 publications

Molecular Characterization, Expression Analysis of Carotenoid, Xanthophyll, Apocarotenoid Pathway Genes, and Carotenoid and Xanthophyll Accumulation in Chelidonium majus L.

Molecular Characterization, Expression Analysis of Carotenoid, Xanthophyll, Apocarotenoid Pathway Genes, and Carotenoid and Xanthophyll Accumulation in Chelidonium majus L.

Modifying Anthocyanins Biosynthesis in Tomato Hairy Roots: A Test Bed for Plant Resistance to Ionizing Radiation and Antioxidant Properties in Space

Characterization of BoaCRTISO Reveals Its Role in Carotenoid Biosynthesis in Chinese Kale

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