Citrate Functionalized Mn₃O₄in Nanotherapy of Hepatic Fibrosis by Oral Administration

Abstract

Aim:To test the potential of orally administered citrate functionalized Mn3O4 nanoparticles (C-Mn3O4 NPs) as a therapeutic agent against hepatic fibrosis and associated chronic liver diseases.Materials & methods:C-Mn3O4 NPs were synthesized and the pH dependent antioxidant mechanism was characterized by in vitro studies. CCl4 intoxicated mice were orally treated with C-Mn3O4 NPs to test its in vivo antioxidant and antifibrotic ability.Results:We demonstrated ultrahigh efficacy of the C-Mn3O4 NPs in treatment of chronic liver diseases such as hepatic fibrosis and cirrhosis in mice compared with conventional medicine silymarin without any toxicological implications.Conclusion:These findings may pave the way for practical clinical use of the NPs as safe medication of chronic liver diseases associated with fibrosis and cirrhosis in human subjects.

A Smart Nanotherapeutic Agent for in vitro and in vivo Reversal of Heavy‐Metal‐Induced Causality: Key Information from Optical Spectroscopy

“…Mice were fasted for 12 h on the 50th day, and blood samples were collected by orbital exsanguination from the retro‐orbital sinus plexus of the mice in micro centrifuge tubes with and without EDTA (2.0 %). Serum was separated following standard procedure and kept at −20 °C until further use.…”

Novel one pot synthesis and spectroscopic characterization of a folate-Mn3O4 nanohybrid for potential photodynamic therapeutic application

“…24-0734). 22,23,29 Absence of any additional peaks from other phases, indicated high purity and good crystallinity of the synthesized material. From the aforementioned ndings we can conclude that the hydrothermal method was able to synthesize highly crystalline Mn 3 O 4 capped with folate.…”

“…To investigate the participation of singlet oxygen radicals, we carried out the DCFH oxidation assay in the presence of a wellknown singlet oxygen quencher, sodium azide. 23,34 The rate of DCFH oxidation (i.e., increase in uorescence intensity) was decreased signicantly in presence of sodium azide (Fig. 3b).…”

“…The aforementioned results clearly indicates the nature of the ROS predominantly to be singlet oxygen rather than superoxide anion, which is consistent with the previously reported mechanistic pathways of ROS generation by surface functionalized manganese oxide nanoparticles. 22,23,35 To elucidate the role of dissolved oxygen in the aforementioned ROS generation pathway, we carried out DCFH oxidation assay aer nitrogen purging for one hour. The reduction in DCFH oxidation implies that the dissolved oxygen in the medium is involved in the generation of singlet oxygen.…”

“…By modulating the surface functionalization of this nanoparticle one can modulate its physicochemical, magnetic and optical properties. 21 Its ROS generation ability with different capping ligands [21][22][23] makes it a potential agent against cancer and associated disorders. In this study we demonstrate a one-step method for the preparation of folic acid-functionalised manganese oxide nanoparticles (FA-Mn 3 O 4 NPs) which have enhanced uptake and toxicity in cancer cells upon blue light irradiation.…”