Citrate-Capped AuNP Fabrication, Characterization and Comparison with Commercially Produced Nanoparticles

Abstract

Gold nanoparticles (AuNPs) were synthesized using citrate reduction, also known as the Turkevich method. The AuNPs were compared with the commercially available product and later subjected to characterization. The AuNPs were 13 nm in diameter with a 2.7 × 108 M−1cm−1 extension coefficient. The calculated concentration was 5.1 nM through the Beer–Lambert law using UV–vis absorbance spectra. Further detailed characterization was applied, such as scanning electron microscopy (SEM), high-resolution transmission electron microscopy (HR-TEM), elemental analysis using electro and DLS instruments, energy-dispersive X-ray spectroscopy (EDS), XRD, and Zeta potential. The synthesized AuNPs had a higher UV-absorbance peak of 0.93 in comparison to commercially available nanoparticles at 5.8 identical conditions. The characterization confirmed successful fabrication of colloidal-citrate-capped AuNPs and their dispersed and aggregated state with induced salt concentration. The shape and morphology were confirmed through XRD, showing a face-centered cubic lattice of {111}, confirmed at 38.1 round shape, and a crystalline lattice. AuNPs tend to be applied in sensing, detection, drug delivery, pharmaceuticals, and other applications in the environment and materials. Other applications include environmental contaminant detection, colorimetric sensors, antimicrobial applications, biosensing and drug delivery, tissue engineering, nanomedicines, optoelectronics, and catalysts.

Fibrillation of Human Serum Albumin Differentially Affected by Asp-, Arg-, and Tyr-Capped Gold Nanoparticles

“…Figure 1 shows the UV−vis spectra of the amino acid functionalized AuNPs. CT-AuNPs show the characteristic LSPR peak at 520 nm, 57 whereas for the Tyr-AuNPs, there is a slight blue shift and the peak is obtained at 518 nm. For Arg-AuNPs, a broad peak with a shoulder arises at ∼621 nm.…”