cis-Jasmone Elicits Aphid-Induced Stress Signalling in Potatoes

Abstract

Elicitation of plant defense signaling that results in altered emission of volatile organic compounds (VOCs) offers opportunities for protecting plants against arthropod pests. In this study, we treated potato, Solanum tuberosum L., with the plant defense elicitor cis-jasmone (CJ), which induces the emission of defense VOCs and thus affects the behavior of herbivores. Using chemical analysis, electrophysiological and behavioral assays with the potato-feeding aphid Macrosiphum euphorbiae, we showed that CJ treatment substantially increased the emission of defense VOCs from potatoes compared to no treatment. Coupled GC-electroantennogram (GC-EAG) recordings from the antennae of M. euphorbiae showed robust responses to 14 compounds present in induced VOCs, suggesting their behavioral role in potato/aphid interactions. Plants treated with CJ and then challenged with M. euphorbiae were most repellent to alate M. euphorbiae. Principal component analysis (PCA) of VOC collections suggested that (E)-2-hexenal, (E,E)-4,8,12-trimethyl-1,3,7,11-tridecatetraene (TMTT), (E)-β-farnesene, (E)-4,8-dimethyl-1,3,7-nonatriene (DMNT), methyl salicylate (MeSA), CJ, and methyl benzoate (MeBA) were the main VOCs contributing to aphid behavioral responses, and that production of TMTT, (E)-β-farnesene, CJ, and DMNT correlated most strongly with aphid repellency. Our findings confirm that CJ can enhance potato defense against aphids by inducing production of VOCs involved in aphid-induced signalling.Electronic supplementary materialThe online version of this article (doi:10.1007/s10886-016-0805-9) contains supplementary material, which is available to authorized users.

Attractiveness of Host Plant Volatile Extracts to the Asian Citrus Psyllid, Diaphorina citri, is Reduced by Terpenoids from the Non-Host Cashew

“…as repellents) against a range of insect pests e.g. aphids, leafhoppers, stinkbugs (Hegde et al 2011; Moraes et al 2009; Oluwafemi et al 2011; Sobhy et al 2017), and in indirect defence via recruitment of natural enemy parasitic wasps (Bruce et al 2008; Li et al 2017; Moraes et al 2009; Tamiru et al 2011). Furthermore, Magalhães et al (2012) suggested that cotton boll weevils, Anthonomus grandis recognize the physiological stage of host cotton plants via information on the amount of DMNT and TMTT released by cotton plants.…”

“…the residence time (time spent in minutes) and the number of entries of D. citri in the treatment and control arms, were subjected to paired t -tests ( P  < 0.05), as described previously (Hegde et al 2011; Sobhy et al 2017). For this, the residence time in the control arms was obtained as a mean value.…”

Association between olfactory sensitivity and behavioral responses of Drosophila suzukii to naturally occurring volatile compounds

“…Excluding cis ‐jasmone with 90% of purity, all compounds were purchased with a purity of ≥99% (GC) and used without further purification. These compounds were chosen on the basis of data available in the literature on different species of invertebrates: eugenol, a phenolic compound used in perfumes, flavorings, and as a local antiseptic and anesthetic (Jadhav, Khandelwal, Ketkar, & Pisal, 2004; Sell & Carlini, 1976), presents a repellent activity (Del Fabbro & Nazzi, 2013; Zeringota et al, 2013); menthol, the main component of peppermint essential oil (Ibanez & Amparo Blazquez, 2018), shows repellent activity (Park et al, 2016; Renkema et al, 2016; Renkema et al, 2017); vanillin, an aromatic compound and flavoring agent, shows the synergic effect with essential oils with repellent activity (Kamsuk et al, 2007); and cis ‐jasmone, a volatile compound that enhances the plant defense against herbivores and insects (Sobhy et al, 2017).…”

“…On the basis of these considerations, the aim of this study was to evaluate peripheral sensitivity of adult females of the spotted‐wing D. suzukii toward volatile compounds, which in other invertebrates and populations of D. suzukii have shown a repellent action (Del Fabbro & Nazzi, 2013; Kamsuk et al, 2007; Munerato, Sinigaglia, Reguly, & Rodrigues de Andrade, 2005; Park, Jang, Yoon, & Kim, 2016; Renkema et al, 2017; Renkema, Wright, Buitenhuis, & Hallett, 2016; Sobhy et al, 2017; Zeringota et al, 2013), by means of an electrophysiological and behavioral approach. In this respect, we evaluated the antennal olfactory sensitivity toward increasing concentrations of repellent compounds (both alone and as binary mixtures) and the subsequent ovipositing behavior evoked in adult females of D. suzukii .…”

“…These compounds are naturally occurring and showed biological activity in different studies aimed at developing control strategies of different pests. In fact, eugenol exhibited repellent action on larvae and adult ticks (Del Fabbro & Nazzi, 2013; Zeringota et al, 2013), and genotoxic effect on D. melanogaster (Munerato et al, 2005); vanillin increased the repellence times of DEET against mosquitoes (Kamsuk et al, 2007) and evoked the strongest antennal responses in adults of Philaenus spumarius (Germinara et al, 2017); menthol displayed moderate repellent action on D. suzukii populations in Canada (Renkema et al, 2016) and Republic of Korea (Park et al, 2016); finally, plants treated with cis ‐jasmone were more repellent to aphid Macrosiphum euphorbiae (Sobhy et al, 2017).…”