CiRS-7 promotes growth and metastasis of esophageal squamous cell carcinoma via regulation of miR-7/HOXB13

Li, Ruichao; Ke, Shun; Meng, Fankai; Lü, Jun; Zou, Xiaojing; He, Zhigang; Wang, Weng-feng; Fang, Minghao

doi:10.1038/s41419-018-0852-y

Cited by 169 publications

(158 citation statements)

References 52 publications

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“…For instance, circ‐Sry contains 16 binding sites of miR‐138 and interacts with miR‐138 . The CDR1as/ciRS‐7 harbours more than 70 binding sites of miR‐7 and could sequester miR‐7 to reduce its tumour‐suppressive roles in colorectal cancer and nasopharyngeal carcinoma . CircHIPK3 was proved to be a tumour‐related molecule in bladder cancer by sequestering miR‐558 .…”

Section: Discussionmentioning

confidence: 99%

Effect of up‐regulation of circMATR3 on the proliferation, metastasis, progression and survival of hypopharyngeal carcinoma

Wang

Wei

et al. 2020

J Cellular Molecular Medi

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Increasing number of circular RNAs (circRNAs) have been reported to play important role in gene regulation, carcinogenesis and pathogenesis in various cancers.However, the biological functions and underlying molecular mechanisms of circRNAs in hypopharyngeal squamous cell carcinoma (HSCC) remain elusive. Thus, secondary circRNA-seq profiling was performed to identify the differentially expressed circR-NAs between HSCC tissues and adjacent normal tissues, and the expression level of circMATR3 (derived from human gene matrin3 (MATR3), has_circRNA_0008922) was confirmed by qRT-PCR. Proliferation of HSCC cells was detected by cell counting kit-8 (CCK8) assay, apoptosis and the cell cycle were analysed by flow cytometry, and the migration and invasion of HSCC cells was determined by transwell assay.Bioinformatics analysis was conducted to predict possible pathways and potential miRNA targets of circMATR3. We found that circMATR3 was up-regulated in HSCC tissues, and abundant circMATR3 expression was markedly correlated with late T classification, advanced clinical stage, greater lymph node metastasis, and poor prognosis. Furthermore, knock-down of circMATR3 significantly inhibited proliferation, migration and invasion of HSCC cells, whereas silencing of circMATR3 induced cell apoptosis. Our analysis predicted that circMATR3 may participate in cancer-related pathways by serving as miRNA sponges. In conclusion, our findings first identified the oncogenic roles of circMATR3 in promoting the progression of HSCC and demonstrated that circMATR3 may be a novel prognostic marker and therapeutic target for HSCC. K E Y W O R D S circMATR3, circular RNAs, hypopharyngeal squamous cell carcinoma, progression S U PP O RTI N G I N FO R M ATI O N Additional supporting information may be found online in the Supporting Information section. How to cite this article: Wang Z, Wei P, Wei D, et al. Effect of up-regulation of circMATR3 on the proliferation, metastasis, progression and survival of hypopharyngeal carcinoma. J Cell

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Section: Discussionmentioning

confidence: 99%

Effect of up‐regulation of circMATR3 on the proliferation, metastasis, progression and survival of hypopharyngeal carcinoma

Wang

Wei

et al. 2020

J Cellular Molecular Medi

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“…In addition, circRNAs have been proposed to play important roles in miRNA function and transcription regulation by acting as eTMs [26,39]. In human, ciRS-7 was reported to be a negative regulator of miR-7 [40]. Many circRNAs that contained miRNA binding sites were also found to be potential eTMs in Arabidopsis, rice and tomato [27,39].…”

Section: Disscusionmentioning

confidence: 99%

Identification and characterization of circular RNAs during the sea buckthorn fruit development

Zhang

Chen

et al. 2019

RNA Biology

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As a rising star of noncoding RNA, circular RNAs (circRNAs) have a covalently closed loop structure, which formed by 3ʹ-5ʹ ligation during splicing. A few circRNAs were identified and thought to be transcriptional noise due to cognitive defect over the past 40 years. Recently, with the development of high-throughput RNA sequencing techniques and specific algorithms for circRNA detection and quantification, plenty of potential circRNAs were identified in many species which play important roles in various biological processes. However, researches on circRNAs in fruit ripening process were lacking. Here, we totally identified 2616 circRNAs in sea buckthorn fruit development process, which uniformly distributed in sea buckthorn chromosome. Among them, 1721 (65.8%) circRNAs were arising from the exons of their host genes, 252 circRNAs were identified as the differentially expressed circRNAs (DEcircRNAs) between three different development stages, and 181 (71.8%) DEcircRNAs had sequence similarity with 235 identified circRNAs from five know plant species. Functional annotation revealed that host genes of DEcircRNAs were predicted to be involved in carotenoid biosynthesis, lipid synthesis and plant hormone signal transduction. Additionally, 53 DEcircRNAs were predicted as the corresponding nine miRNAs sponges in sea buckthorn. Divergent reverse-transcription PCR and RT-qPCR were used for validate the differential expression and back-splicing sites of six DEcircRNAs. These results revealed the role of circRNAs in sea buckthorn fruit ripening process and promoted the noncoding RNA researches in plants.

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“…4 circRNAs, a group of important endogenous non-coding RNAs, mainly consist of transcripts from exons and exert important roles in the downstream gene regulation. 11,12 miRNAs are another group ofnon-coding RNAs containing approximately 22 nucleotides, with a role of regulating gene expression. Thus, compared to their linear counterparts, circRNAs are extraordinarily stable in vivo due to their resistance to exonuclease.…”

Section: Introductionmentioning

confidence: 99%

Circular RNA cMras inhibits lung adenocarcinoma progression via modulating miR‐567/PTPRG regulatory pathway

Tian

et al. 2019

Cell Proliferation

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Objectives: Circular RNA, a type of RNA formed by a covalently closed loop, possesses sophisticated abilities of gene regulation in tumorigenesis and metastasis.However, the role of circRNAs on lung adenocarcinoma (LUAD) remains largely unknown. Materials and methods:The role of cMras was examined both in vitro and in vivo.cMras expression in LUAD tissues was determined by quantitative real-time PCR (qRT-PCR). Downstream targets of cMras were predicted by bioinformatics tools and confirmed by RNA immunoprecipitation assay and luciferase assay. qRT-PCR and western blot assay were used to detect the expression of specific targets.Results: Thirty-six paired LUAD and healthy tissues were collected and cMras resulted significantly downregulated in cancerous tissues. Its expression was negatively associated with tumour stages. cMras overexpression suppressed LUAD growth and metastasis, while endogenous cMras silencing resulted in the opposite effects. Bioinformatics analysis and experimental evidence confirmed that cMras was a sponge of miRNA-567 and released its direct target, PTPRG. cMras overexpression decreased miR-567 expression and subsequently increased PTPRG expression, while increased miRNA-567 expression blocked the effects induced by cMras.Moreover, PTPRG was downregulated in LUAD and patients with low PTPRG expression exhibited significantly poor prognosis. These results suggested that cMras/ miR-567/PTPRG regulatory pathway might be associated to LUAD tumorigenesis and development. Conclusions:A novel circular RNA cMras and its functions were identified, discovering a cMras/miR-567/PTPRG regulatory pathway in LUAD tumorigenesis and development.

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CiRS-7 promotes growth and metastasis of esophageal squamous cell carcinoma via regulation of miR-7/HOXB13

Cited by 169 publications

References 52 publications

Effect of up‐regulation of circMATR3 on the proliferation, metastasis, progression and survival of hypopharyngeal carcinoma

Effect of up‐regulation of circMATR3 on the proliferation, metastasis, progression and survival of hypopharyngeal carcinoma

Identification and characterization of circular RNAs during the sea buckthorn fruit development

Circular RNA cMras inhibits lung adenocarcinoma progression via modulating miR‐567/PTPRG regulatory pathway

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