Circulating Tumor Cells in Metastatic Breast Cancer: Biologic Staging Beyond Tumor Burden

Cristofanilli, Massimo; Broglio, Kristine; Guarneri, Valentina; Jackson, Summer; Fritsche, Herbert A.; Islam, Rabiul; Dawood, Shaheenah; Reuben, James M.; Kau, Shu Wan; Lara, Jonathan F.; Krishnamurthy, Savitri; Ueno, Naoto T.; Hortobágyi, Gabriel N.; Valero, Vicente

doi:10.3816/cbc.2007.n.004

Cited by 155 publications

(128 citation statements)

References 28 publications

Supporting

Mentioning

115

Contrasting

Unclassified

Order By: Relevance

“…Circulating tumor epithelial cells circulate in the peripheral blood and may represent intermediate cells between primary tumors and metastases. Showing promise for improved cancer prognosis, CEpC numbers are reported to better correlate with cancer progression than imaging, lymph node biopsy, and serum biomarker analysis (3,25). In addition to CEpC enumeration, successful molecular analysis of CEpCs-including detecting DNA aberrations, profiling gene expression, or determining degree of phenotype heterogeneity of the cells-should provide candidate surrogate endpoints for evaluation in clinical trials.…”

Section: Discussionmentioning

confidence: 99%

Isolating highly enriched populations of circulating epithelial cells and other rare cells from blood using a magnetic sweeper device

Talasaz

Powell²,

Huber

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

436

298

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Isolating highly enriched populations of circulating epithelial cells and other rare cells from blood using a magnetic sweeper device

Talasaz

Powell²,

Huber

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

436

298

View full text Add to dashboard Cite

“…The research group rested their case stating that CTCs have superior and independent prognostic value. [16] Based on the background that increased CTCs were associated with poor prognosis in terms of PFS and OS, Smerage et al…”

Section: Circulating Tumor Cells In Metastatic Breast Cancermentioning

confidence: 99%

Circulating tumor cells in breast cancer

Jain¹,

Suryavanshi²,

Batra³

et al. 2017

Int J Mol Immuno Oncol.

View full text Add to dashboard Cite

Breast cancer is a heterogeneous disease. Liquid biopsy is a novel diagnostic tool and may provide answers to many questions related to unevenness in prognosis and ultimate outcome. Different technologies for CTC isolation, enrichment, detection, and characterization are under evaluation. Various clinical trials and meta-analysis have been conducted to define the role of CTC in early and metastatic breast cancer. CTCs are superior to other serum markers for prognostication. Their role as predictive marker remains elusive.

show abstract

“…2 The clinical significance of circulating tumor cells (CTCs) in metastatic cancer has been clearly demonstrated. 3,4 The prognostic value of CTCs drug resistance profile in metastatic breast cancer patients has been confirmed, 5 and the detection of CTCs before initiation of therapy in cancer patients with a) Author to whom correspondence should be addressed. Electronic mail: zohar@email.arizona.edu.…”

Section: Introductionmentioning

confidence: 99%

“…25,26 In particular, antibody-functionalized microchannels have been utilized for the isolation of cancer cells from either homogeneous or heterogeneous suspensions. 4,27,28 Utilizing micro-posts coated with EpCAM antibodies, viable CTCs were selectively separated from peripheral whole blood samples. 27 By combining E-selectin and anti-EpCAM molecules, efficient capture of target cells was reported in microfluidic chambers.…”

Section: Introductionmentioning

confidence: 99%

Isolation of viable cancer cells in antibody-functionalized microfluidic devices

et al. 2014

View full text Add to dashboard Cite

Microfluidic devices functionalized with EpCAM antibodies were utilized for the capture of target cancer cells representing circulating tumor cells (CTCs). The fraction of cancer cells captured from homogeneous suspensions is mainly a function of flow shear rate, and can be described by an exponential function. A characteristic shear rate emerges as the most dominant parameter affecting the cell attachment ratio. Utilizing this characteristic shear rate as a scaling factor, all attachment ratio results for various combinations of receptor and ligand densities collapsed onto a single curve described by the empirical formula. The characteristic shear rate increases with both cell-receptor and surface-ligand densities, and empirical formulae featuring a product of two independent cumulative distributions described well these relationships. The minimum detection limit in isolation of target cancer cells from binary mixtures was experimentally explored utilizing microchannel arrays that allow high-throughput processing of suspensions about 0.5 ml in volume, which are clinically relevant, within a short time. Under a twostep attachment/detachment flow rate, both high sensitivity (almost 1.0) and high specificity (about 0.985) can be achieved in isolating target cancer cells from binary mixtures even for the lowest target/non-target cell concentration ratio of 1:100 000; this is a realistic ratio between CTCs and white blood cells in blood of cancer patients. Detection of CTCs from blood samples was also demonstrated using whole blood from healthy donors spiked with cancer cells. Finally, the viability of target cancer cells released after capture was confirmed by observing continuous cell growth in culture. V C 2014 AIP Publishing LLC.

show abstract

Circulating Tumor Cells in Metastatic Breast Cancer: Biologic Staging Beyond Tumor Burden

Cited by 155 publications

References 28 publications

Isolating highly enriched populations of circulating epithelial cells and other rare cells from blood using a magnetic sweeper device

Isolating highly enriched populations of circulating epithelial cells and other rare cells from blood using a magnetic sweeper device

Circulating tumor cells in breast cancer

Isolation of viable cancer cells in antibody-functionalized microfluidic devices

Contact Info

Product

Resources

About