Circulating miRNAs miR-574-5p and miR-3135b are potential metabolic regulators for serum lipids and blood glucose in gestational diabetes mellitus

Wang, Fuyan; Li, Zhulin; Zhao, Ming; Ye, Wen; Wu, Hao; Liao, Qi; Bu, Shizhong; Zhang, Yisheng

doi:10.1080/09513590.2021.1908990

Cited by 22 publications

(15 citation statements)

References 34 publications

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“…Furthermore, some of the miRNAs reported herein have been previously associated with other pathological conditions. For instance, hsa-miR-451a has been reported as a tumor suppressor in gastric cancer [ 36 ] and hepatocellular carcinoma [ 37 ], hsa-miR-574-5p has been proposed as a biomarker for non-small cell lung cancer [ 38 ] and as a metabolic regulator in gestational diabetes mellitus [ 39 ], while dysregulation of hsa-miR-6721-5p, hsa-miR-142-3p, and hsa-miR-149-5p has been reported in Alzheimer’s disease [ 40 ].…”

Section: Discussionmentioning

confidence: 99%

Identification of Novel Circulating miRNAs in Patients with Acute Ischemic Stroke

Al-Dous

Toor

Parray

et al. 2022

IJMS

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Ischemic strokes are associated with significant morbidity and mortality, but currently there are no reliable prognostic or diagnostic blood biomarkers. MicroRNAs (miRNAs) regulate various molecular pathways and may be used as biomarkers. Using RNA-Seq, we conducted comprehensive circulating miRNA profiling in patients with ischemic stroke compared with healthy controls. Samples were collected within 24 h of clinical diagnosis. Stringent analysis criteria of discovery (46 cases and 95 controls) and validation (47 cases and 96 controls) cohorts led to the identification of 10 differentially regulated miRNAs, including 5 novel miRNAs, with potential diagnostic significance. Hsa-miR-451a was the most significantly upregulated miRNA (FC; 4.8, FDR; 3.78 × 10−85), while downregulated miRNAs included hsa-miR-574-5p and hsa-miR-142-3p, among others. Importantly, we computed a multivariate classifier based on the identified miRNA panel to differentiate between ischemic stroke patients and healthy controls, which showed remarkably high sensitivity (0.94) and specificity (0.99). The area under the ROC curve was 0.97 and it is superior to other current available biomarkers. Moreover, in samples collected one month following stroke, we found sustained upregulation of hsa-miR-451a and downregulation of another 5 miRNAs. Lastly, we report 3 miRNAs that were significantly associated with poor clinical outcomes of stroke, as defined by the modified Rankin scores. The clinical translation of the identified miRNA panel may be explored further.

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Section: Discussionmentioning

confidence: 99%

Identification of Novel Circulating miRNAs in Patients with Acute Ischemic Stroke

Al-Dous

Toor

Parray

et al. 2022

IJMS

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“…Stirm et al (60) reported conflicting results on the expression of let-7g. These authors reported higher expression of let-7g in the screening group, however, no difference was observed in the validation group in whole blood samples of German women with GDM compared to controls in the screening group (60) Other articles included in this review reported differential miRNA expression, yet these miRNAs were identified in single studies only (36,37,43,44,48,50,57,59,69,71,74,76,77,80,81).…”

Section: Qualitative Synthesis Of Studiesmentioning

confidence: 87%

A Big Role for microRNAs in Gestational Diabetes Mellitus

et al. 2022

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Maternal diabetes is associated with pregnancy complications and poses a serious health risk to both mother and child. Growing evidence suggests that pregnancy complications are more frequent and severe in pregnant women with pregestational type 1 diabetes mellitus (T1DM) and type 2 diabetes mellitus (T2DM) compared to women with gestational diabetes mellitus (GDM). Elucidating the pathophysiological mechanisms that underlie the different types of maternal diabetes may lead to targeted strategies to prevent or reduce pregnancy complications. In recent years, microRNAs (miRNAs), one of the most common epigenetic mechanisms, have emerged as key players in the pathophysiology of pregnancy-related disorders including diabetes. This review aims to provide an update on the status of miRNA profiling in pregnancies complicated by maternal diabetes. Four databases, Pubmed, Web of Science, EBSCOhost, and Scopus were searched to identify studies that profiled miRNAs during maternal diabetes. A total of 1800 articles were identified, of which 53 are included in this review. All studies profiled miRNAs during GDM, with no studies on miRNA profiling during pregestational T1DM and T2DM identified. Studies on GDM were mainly focused on the potential of miRNAs to serve as predictive or diagnostic biomarkers. This review highlights the lack of miRNA profiling in pregnancies complicated by T1DM and T2DM and identifies the need for miRNA profiling in all types of maternal diabetes. Such studies could contribute to our understanding of the mechanisms that link maternal diabetes type with pregnancy complications.

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“…The lncRNA microarray analysis revealed that lncRNAs ERMP1, TSPAN32, and MRPL38 form a coexpression network with TPH1, which is mainly involved in the tryptophan metabolism pathway, and lncRNA RPL13P5 forms a coexpression network with the TSC2 gene via the pi3k-akt and insulin signaling pathways (29). The miRNA expression profiles of plasma samples revealed that miR-574-5p and miR-3135b may serve as metabolic regulators of glucose and lipid levels in GDM (30).…”

Section: Discussionmentioning

confidence: 99%

Upregulation of T Cell Receptor Signaling Pathway Components in Gestational Diabetes Mellitus Patients: Joint Analysis of mRNA and circRNA Expression Profiles

Chen

Cai²,

Zhao

et al. 2022

Front. Endocrinol.

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ObjectiveGestational diabetes mellitus (GDM) is one of the most common complications of pregnancy, and its pathogenesis is still unclear. Studies have shown that circular RNAs (circRNAs) can regulate blood glucose levels by targeting mRNAs, but the role of circRNAs in GDM is still unknown. Therefore, a joint microarray analysis of circRNAs and their target mRNAs in GDM patients and healthy pregnant women was carried out.MethodsIn this study, microarray analyses of mRNA and circRNA in 6 GDM patients and 6 healthy controls were conducted to identify the differentially expressed mRNA and circRNA in GDM patients, and some of the discovered mRNAs and circRNAs were further validated in additional 56 samples by quantitative realtime PCR (qRT-PCR) and droplet digital PCR (ddPCR).ResultsGene ontology and pathway analyses showed that the differentially expressed genes were significantly enriched in T cell immune-related pathways. Cross matching of the differentially expressed mRNAs and circRNAs in the top 10 KEGG pathways identified 4 genes (CBLB, ITPR3, NFKBIA, and ICAM1) and 4 corresponding circRNAs (circ-CBLB, circ-ITPR3, circ-NFKBIA, and circ-ICAM1), and these candidates were subsequently verified in larger samples. These differentially expressed circRNAs and their linear transcript mRNAs were all related to the T cell receptor signaling pathway, and PCR results confirmed the initial microarray results. Moreover, circRNA/miRNA/mRNA interactions and circRNA-binding proteins were predicted, and circ-CBLB, circ-ITPR3, and circ-ICAM1 may serve as GDM-related miRNA sponges and regulate the expression of CBLB, ITPR3, NFKBIA, and ICAM1 in cellular immune pathways.ConclusionUpregulation of T cell receptor signaling pathway components may represent the major pathological mechanism underlying GDM, thus providing a potential approach for the prevention and treatment of GDM.

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Circulating miRNAs miR-574-5p and miR-3135b are potential metabolic regulators for serum lipids and blood glucose in gestational diabetes mellitus

Cited by 22 publications

References 34 publications

Identification of Novel Circulating miRNAs in Patients with Acute Ischemic Stroke

Identification of Novel Circulating miRNAs in Patients with Acute Ischemic Stroke

A Big Role for microRNAs in Gestational Diabetes Mellitus

Upregulation of T Cell Receptor Signaling Pathway Components in Gestational Diabetes Mellitus Patients: Joint Analysis of mRNA and circRNA Expression Profiles

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