Circulating endothelial cells as markers for ANCA-associated small-vessel vasculitis

Woywodt, Alexander; Streiber, Frank; Groot, Kirsten de; Regelsberger, Heide; Haller, Hermann; Haubitz, Marion

doi:10.1016/s0140-6736(03)12269-6

Cited by 261 publications

(275 citation statements)

References 21 publications

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“…This is not surprising, because for both lcSSc and dcSSc several studies have demonstrated widespread vascular damage in lesional as well as prelesional tissues (5,29,30). Moreover, the amount of CECs found in SSc is comparable with that reported in patients with different diseases characterized by very extensive vascular injury, such as sickle cell anemia, rickettsial and cytomegalovirus infections, and antineutrophil cytoplasmic antibody-associated vasculitides (9,11,12,15). The fact that SSc (among the vasculopathies described in other connective tissue diseases) is characterized by peculiar and particularly aggressive endothelial damage is further confirmed by our preliminary results obtained in patients with SLE.…”

Section: Discussionmentioning

confidence: 76%

“…However, the amount of CECs detectable in peripheral blood has been recently proposed as a reliable marker of endothelial damage in different vascular diseases, such as acute coronary syndrome (also after coronary angioplasty), sickle cell anemia, thrombotic thrombocytopenic purpura, rickettsial and cytomegalovirus infections, Behçet's disease, SLE, and small-vessel vasculitides (7)(8)(9)(10)(11)(12)(13)(14)(15). Thus, the detection of CECs in patients with SSc is certainly not surprising, because results of several histologic studies of dermal microvas- culature in this disease, which demonstrated disruption of the normal architecture of the endothelium with loss of intercellular junctions and progressive death of endothelial cells (3)(4)(5)(6), have suggested that endothelium may play a key role in the pathologic process of SSc.…”

Section: Discussionmentioning

confidence: 99%

“…In humans, CECs have been detected in diverse conditions having in common endothelial damage, such as coronary angioplasty, acute coronary syndrome, sickle cell anemia, thrombotic thrombocytopenic purpura, infection with Rickettsia conorii or cytomegalovirus, Behçet's disease, systemic lupus erythematosus (SLE), and small-vessel vasculitis (7)(8)(9)(10)(11)(12)(13)(14)(15). Moreover, it has been suggested that in response to severe ischemia or cytokine stimuli, circulating endothelial cell progenitors (CEPs) increase and home into sites of angiogenesis and/or vascular damage, and consequently contribute to neovascularization and/or wound-healing processes (16)(17)(18).…”

Section: Conclusion the Presence Of Cecs In Patientsmentioning

confidence: 99%

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Circulating endothelial cells as a marker of ongoing vascular disease in systemic sclerosis

Papa

Colombo

Fracchiolla

et al. 2004

Arthritis & Rheumatism

136

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Results. Total and activated CEC counts were significantly higher in SSc patients compared with healthy controls and were positively correlated with the disease activity score. With respect to visceral involvement, significant correlation was observed between the CEC number and the severity of pulmonary hypertension. High levels of endothelial progenitors were observed in patients with SSc, and the counts were higher in the early stages of disease.

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Section: Discussionmentioning

confidence: 76%

Section: Discussionmentioning

confidence: 99%

Section: Conclusion the Presence Of Cecs In Patientsmentioning

confidence: 99%

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Circulating endothelial cells as a marker of ongoing vascular disease in systemic sclerosis

Papa

Colombo

Fracchiolla

et al. 2004

Arthritis & Rheumatism

136

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“…Endothelial cells (ECs) can detach from the vascular wall and circulate in the bloodstream, and would then be termed CECs. It has long been shown that the level of CECs is significantly higher in patients with widespread vascular damage, such as antineutrophil cytoplasmic antibody‐associated small vessel vasculitis (Woywodt, Streiber, et al., 2003), sickle cell crisis (Belcher et al., 2003), and pulmonary hypertension (Bull et al., 2003). Therefore, CECs have been used as a marker of endothelial damage in a variety of vascular disorders (Alessio et al., 2013; Davignon & Ganz, 2004; Dignat‐George & Sampol, 2000).…”

Section: Discussionmentioning

confidence: 99%

Circulating endothelial progenitor cells and endothelial cells in moyamoya disease

et al. 2018

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IntroductionThere is no well‐recognized biomarker for accurately predicting outcome in the presence of moyamoya disease (MMD), a progressive occlusive cerebrovascular disease of the internal carotid arteries or their branches. The aim of this study was to investigate the presence of endothelial progenitor cells (EPCs) and circulating endothelial cells (CECs) in MMD and correlate the findings with clinical features.MethodsPatients with MMD (n = 66) were compared with healthy controls (n = 81). Blood samples were obtained from an antecubital vein and analyzed using flow cytometry. EPCs were defined as CD31+ CD45dim CD34br CD133+ and CECs as CD31br CD45− CD34dim CD133−. Univariate and multivariate linear regression analyses were carried out.ResultsThe CEC counts were significantly higher in the patients than in the controls (p = 0.008). In multivariate analysis, EPC counts were independently associated with age of patients with MMD (p = 0.049) and CEC counts were independently negatively associated with concomitant disease such as hypertension, diabetes mellitus, and coronary heart disease (p = 0.034).ConclusionsThis is the first study to investigate the presence of CECs in the plasma of patients with MMD, and the amount of CECs was negatively correlated with concomitant disease in these patients.

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“…4 An increased number of circulating endothelial cells (CECs) has previously been reported after the onset of various diseases that are associated with endothelial injury, including myocardial infarction, septic shock, sickle cell crisis, antineutrophil cytoplasmic autoantibody (ANCA)-associated small vessel vasculitis, thrombotic thrombocytopenic purpura (TTP), systemic lupus erythematosus and Behçet's disease. [5][6][7][8] An increase in the number of CECs has been used for assessment of vascular damage and it seems to be a marker of disease activity in recent years. 6,8 Hyperhomocysteinemia is now widely recognized as an independent risk factor for cardiovascular disease -homocysteine level was found to be increased and was associated with cellular fibronectin in preeclampsia.…”

Section: Introductionmentioning

confidence: 99%

Circulating endothelial cells in preeclampsia

et al. 2007

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Endothelial dysfunction plays an important role in the pathogenesis of preeclampsia. Increased number of circulating endothelial cells (CECs) have previously been reported after various diseases associated endothelial injury. The aim of this study was to evaluate the CECs in patients with preeclampsia and to demonstrate any association between CECs and homocysteine, which is another marker of vascular injury. The study included 20 preeclamptic, 15 hypertensive women, 15 healthy pregnant and 15 healthy non-pregnant women. All subjects had normal renal function. Systolic and diastolic blood pressures, serum homocysteine levels were measured. To isolate CECs, peripheral blood was first incubated with anti-CD-146 antibody and subsequently conjugated to immunomagnetic beads. Cells were stained with acridine and counted. Preeclamptic patients had elevated numbers of CECs (13.275.2 cells/ml) compared with hypertensive patients (6.970.8 cells/ml), healthy pregnants (5.271.4 cells/ml) and non-pregnant controls (4.071.8 cells/ml), (Po0.0001). Serum homocysteine level in preeclamptic patients (9.572.8 lmol/l) was significantly higher compared with healthy pregnants (6.070.6 lmol/l), was not different from hypertensive patients (11.572.3 lmol/l, P40.05), but it was lesser compared with non-pregnant controls (12.273.3 lmol/l, Po0.0001). Also, significant correlation between CECs and systolic blood pressure (Po0.0001, r ¼ 0.63), diastolic blood pressure (Po0.0001, r ¼ 0.64) and serum homocysteine (Po0.01, r ¼ 0.55) levels were found in preeclamptic patients. CECs as a marker of endothelial injury were significantly higher in patients with preeclampsia than in hypertensive patients, healthy pregnants and normal controls. Further studies are needed for the prognostic and potential importance of CECs in preeclampsia.

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Circulating endothelial cells as markers for ANCA-associated small-vessel vasculitis

Cited by 261 publications

References 21 publications

Circulating endothelial cells as a marker of ongoing vascular disease in systemic sclerosis

Circulating endothelial cells as a marker of ongoing vascular disease in systemic sclerosis

Circulating endothelial progenitor cells and endothelial cells in moyamoya disease

Circulating endothelial cells in preeclampsia

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