Circulating Cell-Free DNA: A Promising Marker of Pathologic Tumor Response in Rectal Cancer Patients Receiving Preoperative Chemoradiotherapy

Agostini, Marco; Pucciarelli, Salvatore; Enzo, Maria Vittoria; Bianco, Paola Del; Briarava, Marta; Bedin, Chiara; Friso, Maria Luisa; Lonardi, Sara; Mescoli, Claudia; Toppan, Paola; Urso, E.; Nitti, Donato

doi:10.1245/s10434-011-1638-y

Cited by 121 publications

(123 citation statements)

References 28 publications

Supporting

Mentioning

101

Contrasting

Unclassified

Order By: Relevance

“…In addition, larger lengths of cell-free DNA and the ratio of larger fragments both increased in the higher TRG scores group, which suggested that these parameters were associated with a better response to preoperative chemoradiotherapy. However, this is not consistent with a previous study, in which lower levels of circulating DNA and lower integrity were found in the responsive group, although this was not statistically significant (16). This difference suggested circulating plasma DNA may originate mainly from tumor necrosis but not active liberation of the tumor itself in rectal cancer.…”

Section: Discussioncontrasting

confidence: 99%

“…Circulating cell-free DNA fragments from tumor necrosis were variable in size and generally larger than 200 bp. In addition, the ratio between longer fragments and shorter fragments, which was termed the integrity index, was more reliable in reflecting tumor status (16). In the present study, we also demonstrated that plasma cell-free DNA levels were higher in patients with rectal cancer than in healthy individuals, which was consistent with results of previous reports (31).…”

Section: Discussionsupporting

confidence: 92%

“…In addition, a previous study demonstrated that plasma cell-free DNA concentration showed dynamic changes during radiotherapy (15). Another study revealed that baseline DNA integrity and variations induced by treatment were closely related to pathologic tumor response (16). These data suggest that plasma cell-free DNA detection may have potential clinical utility in cancer treatment.…”

Section: Introductionmentioning

confidence: 81%

See 2 more Smart Citations

The role of plasma cell-free DNA detection in predicting preoperative chemoradiotherapy response in rectal cancer patients

Sun

Zhu

et al. 2013

Oncology Reports

View full text Add to dashboard Cite

Abstract. In the present study, we studied the relationship between plasma cell-free DNA and the effect of preoperative chemoradiotherapy in patients with rectal cancer. The concentration, KRAS mutation and O6-methylguanine-DNA methyltransferase (MGMT) promoter methylation status of cell-free DNA were measured by using polymerase chain reaction (PCR) analyses. The response to chemoradiotherapy was assessed using tumor regression grading (TRG) scores. The cell-free DNA concentrations in patients with rectal cancer (n=34) were significantly higher compared to healthy controls (n=10). The 400-base pair (bp) DNA concentration, 400-/100-bp DNA ratio decreased significantly after chemoradiotherapy in the good response group. The incidence of KRAS mutation decreased significantly after chemoradiotherapy in both good and poor response groups. Higher MGMT promoter methylation status at baseline DNA was associated with a better tumor response. Therefore, cell-free DNA detection may be useful in evaluating the effect of preoperative chemoradiotherapy in patients with rectal cancer.

show abstract

Section: Discussioncontrasting

confidence: 99%

Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 81%

See 1 more Smart Citation

The role of plasma cell-free DNA detection in predicting preoperative chemoradiotherapy response in rectal cancer patients

Sun

Zhu

et al. 2013

Oncology Reports

View full text Add to dashboard Cite

show abstract

“…Patients who responded to chemoradiotherapy, their pre and post treatment cell free DNA integrity indices were found to be statistically different whereas patients who did not respond, their pre and post treatment cell free DNA integrity indices did not differ [54]. Chan et al [55] found that DNA integrity index not only got reduced in patients with nasopharyngeal carcinoma who responded successfully to radiotherapy but was also found to be associated with reduced probability of disease free survival at higher values.…”

Section: Circulating Dna Integrity Index and Its Clinical Significancementioning

confidence: 99%

“…They suggested that integrity index can be a simple and inexpensive way to detect gynecologic and breast cancers. Briefly, integrity index is calculated by amplifying Alu-sequences [48] [54] and nasopharyngeal cancers [55]. Interestingly, in a review focused on circulating DNA integrity index, authors have highlighted the fact that DNA integrity index is more sensitive and specific than circulating DNA concentration in differentiating cancer patients from their respective non-neoplastic diseases and healthy controls [56].…”

Section: Circulating Dna Integrity Index and Its Clinical Significancementioning

confidence: 99%

Circulating DNA in Cancer: An Overview

Singh¹,

Saraya²

2015

Chemotherapy (Los Angel)

View full text Add to dashboard Cite

In the last decade, research has been focused on discovering markers which have high sensitivity and specificity to detect cancer. Molecular biologists have been trying to find markers which can be detected non-invasively. Circulating nucleic acids in plasma and serum of cancer patients and the mutations detected in them have gained immense importance in this field. Circulating nucleic acids (CNAs) include DNA, RNA, nucleosomal DNA, microRNA, viral DNA. This review focuses on circulating DNA, its origin and mechanism of release, its diagnostic and prognostic utility, association with any of the clinic pathological parameters and its role in monitoring treatment efficiency in cancer. However, due to lack of uniformity in laboratory techniques, variability in disease advancement, less number of samples in various studies, lack of evidences for the origin of circulating nucleic acids, the knowledge sequestered till now in this field has not been translated in to clinical practice. With more high throughput techniques, circulating nucleic acid levels and mutations in them may give rise to a new era of cancer diagnostics and therapy.

show abstract

Association of Observed Perioperative Cell-Free DNA Dynamics With Early Recurrence in Patients With Colon Cancer

et al. 2020

View full text Add to dashboard Cite

whereas the mean age of the general anesthesia cohort did not (b = 0.0557; P = .47).Discussion | This analysis demonstrates increased use of spinal anesthesia for the repair of hip fractures over the study period, 2007 to 2017. This finding may be explained by the observed demographic shift, with increasing age in the spinal anesthesia cohort (but not the general anesthesia cohort) paralleling the rise in use of spinal anesthesia.Because the purported benefits of spinal anesthesia in patients with hip fractures are associated with the minimization of interventional morbidity in frail, older patients, this analysis supports the notion that use of spinal anesthesia has increased because of growth of this subpopulation, as opposed to the notion that practice patterns have shifted (eg, anesthesiologists using spinal anesthesia with greater frequency overall and/or expanding its use to younger or less frail patient subpopulations).This analysis has the limitations of any observational study, which by definition cannot demonstrate causation. How-ever, the observed trends are valuable evidence in considering the health care services implications of the noted increase in the use of spinal anesthesia. Results from randomized clinical trials are needed to determine whether increased application of spinal anesthesia to patients beyond the oldest and most frail subgroups is warranted.

show abstract

Circulating Cell-Free DNA: A Promising Marker of Pathologic Tumor Response in Rectal Cancer Patients Receiving Preoperative Chemoradiotherapy

Abstract: The plasma levels of the longer fragments (Alu 247) of cfDNA and the cfDNA integrity index are promising markers to predict tumor response after preoperative CRT for rectal cancer.

Cited by 121 publications

References 28 publications

The role of plasma cell-free DNA detection in predicting preoperative chemoradiotherapy response in rectal cancer patients

The role of plasma cell-free DNA detection in predicting preoperative chemoradiotherapy response in rectal cancer patients

Circulating DNA in Cancer: An Overview

Association of Observed Perioperative Cell-Free DNA Dynamics With Early Recurrence in Patients With Colon Cancer

Contact Info

Product

Resources

About