Circular RNA FLNA acts as a sponge of miR-486-3p in promoting lung cancer progression via regulating XRCC1 and CYP1A1

Pan, Jiongwei; Huang, Gang; Yin, Zhangyong; Cai, Xiaoping; Gong, Enhui; Li, Yuling; Xu, Cunlai; Ye, Zaiting; Cao, Zhuo; Cheng, Wei

doi:10.1038/s41417-021-00293-w

Cited by 16 publications

(13 citation statements)

References 36 publications

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“…Although numerous studies have reported that miRNA expression is altered upon irradiation, studies exploring the mechanisms underlying these miRNA changes in response to irradiation are scarce. Recently, it has been reported that circular RNA can act as a sponge for miRNA to influence its function [ 37 , 38 , 39 ]. Thus, we attempted to look for links that might regulate miRNA production, starting from the miRNA production pathway.…”

Section: Discussionmentioning

confidence: 99%

Irradiation Activates MZF1 to Inhibit miR-541-5p Expression and Promote Epithelial-Mesenchymal Transition (EMT) in Radiation-Induced Pulmonary Fibrosis (RIPF) by Upregulating Slug

Liang

Yan²,

Wang³

et al. 2021

IJMS

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Understanding miRNAs regulatory roles in epithelial-mesenchymal transition (EMT) would help establish new avenues for further uncovering the mechanisms underlying radiation-induced pulmonary fibrosis (RIPF) and identifying preventative and therapeutic targets. Here, we demonstrated that miR-541-5p repression by Myeloid Zinc Finger 1 (MZF1) promotes radiation-induced EMT and RIPF. Irradiation could decrease miR-541-5p expression in vitro and in vivo and inversely correlated to RIPF development. Ectopic miR-541-5p expression suppressed radiation-induced-EMT in vitro and in vivo. Knockdown of Slug, the functional target of miR-541-5p, inhibited EMT induction by irradiation. The upregulation of transcription factor MZF1 upon irradiation inhibited the expression of endogenous miR-541-5p and its primary precursor (pri-miR-541-5p), which regulated the effect of the Slug on the EMT process. Our finding showed that ectopic miR-541-5p expression mitigated RIPF in mice by targeting Slug. Thus, irradiation activates MZF1 to downregulate miR-541-5p in alveolar epithelial cells, promoting EMT and contributing to RIPF by targeting Slug. Our observation provides further understanding of the development of RIPF and determines potential preventative and therapeutic targets.

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Section: Discussionmentioning

confidence: 99%

Irradiation Activates MZF1 to Inhibit miR-541-5p Expression and Promote Epithelial-Mesenchymal Transition (EMT) in Radiation-Induced Pulmonary Fibrosis (RIPF) by Upregulating Slug

Liang

Yan²,

Wang³

et al. 2021

IJMS

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“… 30 Pan et al used a mouse lung cancer model to find that circFLNA promoted the proliferation, migration, and invasion of lung cancer cells through the sponge effect of miR‐486‐3p; the circFLNA‐miR‐486‐3p‐XRCC1/CYP1A1 regulatory axis was established through subsequent mechanistic experiments. 31 Gao et al screened the plasma of patients with persistent atrial fibrillation by circRNA microarray and found that hsa_circ_0004104 was significantly downregulated; hsa_circ_0004104 negatively correlated with TGF‐β1, suggesting the potential of hsa_circ_0004104 as a biomarker for persistent AF. 32 …”

Section: Discussionmentioning

confidence: 99%

“…the circFLNA-miR-486-3p-XRCC1/CYP1A1 regulatory axis was established through subsequent mechanistic experiments. 31 Gao et al…”

Section: Positioning and Sponge Function Of Hsa_ Circ_0000437mentioning

confidence: 99%

Hsa_circ_0000437 upregulates and promotes disease progression in rheumatic valvular heart disease

Zhu

Wang

et al. 2021

Clinical Laboratory Analysis

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Background: Currently, the diagnosis and outcome of rheumatic valvular heart disease (RVHD) are less than ideal, and there are no accurate biomarkers. Circular RNA (circRNA) might participate in the occurrence and development of RVHD. Materials and methods:We use circRNA microarray to filter out the target has_circ_0000437. qRT-PCR was used to measure the expression levels of hsa_ circ_0000437 in RVHD plasma samples. We assessed the diagnostic value of hsa_ circ_0000437 in RVHD. Cell function in vitro experiment was to explore the effect of has_circ_0000437 on RVHD.Results: Has_circ_0000437 is highly expressed in RVHD (p < 0.001). has_ circ_0000437 has the diagnostic value in RVHD. In RVHD, hsa_circ_0000437 can promote cell proliferation and migration but inhibits its apoptosis. This may be due to the combination of has_circ_0000437 and target miRNA in the cytoplasm that affects the progress of RVHD. Conclusions:Has_circ_0000437 can promote the process of RVHD and may be a potential for the diagnosis and treatment of RVHD.

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“…CircRNAs are considered promising biomarkers and therapeutic targets because of their sequence conservation, biostability, and tissue-specificity [ 17 , 18 ]. Among them, circFLNA is a newly discovered gene [ 19 ], and some studies revealed that overexpression of circFLNA, XRCC1, and CYP1A1 can promote survival and malignant development of cancer cells [ 20 ]. However, what effects circFLNA have on BCa remains to be determined, and given this uncertainty, this study regarding this field has carried out.…”

Section: Discussionmentioning

confidence: 99%

Circular RNA circFLNA inhibits the development of bladder carcinoma through microRNA miR-216a-3p/BTG2 axis

et al. 2021

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Recent studies have shown that circular RNA circFLNA is abnormally expressed in a variety of malignant tumors, but its role and mechanism in bladder carcinoma (BCa) are still unclear. The present paper aims to contribute to research on the effects and mechanism of circFLNA on the malignant phenotype of BCa. In this study, the expressions of circFLNA, miR-216a-3p and BTG2 in BCa and BCa cells (EJ, T24, 5637, TCC-SUP) were detected by qRT-PCR. EdU staining, colony formation, Transwell assay, wound healing assays, and sphere formation assay were used to measure the cell proliferation, viability, invasion, migration, and cell stemness of BCa cells after circFLNA overexpression. In addition, the correlation existed between miR-216a-3p and circFLNA or BTG2 was confirmed by Dual-Luciferase Reporter assay and RNA pull-down. Western blot was utilized to determine the expression of BTG2, MMP2, epithelial-mesenchymal transition (EMT)-related proteins (vimentin, E-cadherin) and stem cell-specific proteins (CD34, OCT4, SOX2). Our study confirmed that downregulated circFLNA and BTG2 expression and upregulated miR-216a-3p were found in both BCa tissues and cell lines. Meanwhile, upregulated circFLNA inhibited proliferation, invasion and migration, EMT and stemness of BCa cells. MiR-216a-3p was a target gene of circFLNA and could target BTG2. Further analysis finally demonstrated that circFLNA sponged miR-216a-3p and indirectly promoted BTG2 expression, ultimately regulating proliferation, migration, invasion and EMT of BCa cells. In conclusion, circFLNA inhibits the malignant phenotype of BCa cells and their stemness through miR-216a-3p/BTG2, thus suppressing BCa progression.

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Circular RNA FLNA acts as a sponge of miR-486-3p in promoting lung cancer progression via regulating XRCC1 and CYP1A1

Cited by 16 publications

References 36 publications

Irradiation Activates MZF1 to Inhibit miR-541-5p Expression and Promote Epithelial-Mesenchymal Transition (EMT) in Radiation-Induced Pulmonary Fibrosis (RIPF) by Upregulating Slug

Irradiation Activates MZF1 to Inhibit miR-541-5p Expression and Promote Epithelial-Mesenchymal Transition (EMT) in Radiation-Induced Pulmonary Fibrosis (RIPF) by Upregulating Slug

Hsa_circ_0000437 upregulates and promotes disease progression in rheumatic valvular heart disease

Circular RNA circFLNA inhibits the development of bladder carcinoma through microRNA miR-216a-3p/BTG2 axis

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