Circular RNA circSATB2 promotes progression of non-small cell lung cancer cells

Abstract

Background: Lung cancer has high morbidity and mortality worldwide with non-small cell lung cancer (NSCLC) accounting for 85% of the cases. Therapies for lung cancer have relatively poor outcomes and further improvements are required. Circular RNAs have been reported to participate in the occurrence and progression of cancer. Information on the functions and mechanism of circRNAs in lung cancer is limited and needs more exploration. Methods: We detected expression of genes and proteins by qPCR and western blot. Function of circSATB2 was investigated using RNA interference and overexpression assays. Location of circSATB2 was assessed by fluorescence in situ hybridization (FISH). Interaction of circSATB2, miR-326 and FSCN1 was confirmed by dual-luciferase reporter assay. Results: Data from the investigation showed that circSATB2 was highly expressed in NSCLC cells and tissues. circSATB2 positively regulated fascin homolog 1, actin-bundling protein 1 (FSCN1) expression via miR-326 in lung cancer cells. Furthermore, circSATB2 can be transferred by exosomes and promote the proliferation, migration and invasion of NSCLC cells, as well as induce abnormal proliferation in normal human bronchial epithelial cells. Also, circSATB2 was highly expressed in serumal exosomes from lung cancer patients with high sensitivity and specificity for clinical detection and was related to lung cancer metastasis. Conclusions: circSATB2 participated in the progression of NSCLC and was differentially expressed in lung cancer tissue and serumal exosomes. circSATB2 may be potential biomarker for the diagnosis of NSCLC.

<p>Circ_SATB2 Attenuates the Anti-Tumor Role of Celastrol in Non-Small-Cell Lung Carcinoma Through Targeting miR-33a-5p/E2F7 Axis</p>

“…25 Zhang et al found that circ_SATB2 accelerated NSCLC progression through acting as miR-326 sponge to up-regulate FSCN1 expression. 15 Here, we found that circ_SATB2 was highly expressed in NSCLC tissues and cell lines. Subsequently, we investigated if Cela suppressed NSCLC progression by reducing circ_SATB2 expression.…”

“…14 As for circRNA SATB homeobox 2 (circ_SATB2), Zhang et al demonstrated that circ_SATB2 accelerated NSCLC development. 15 However, the working mechanism of circ_SATB2 in NSCLC progression remains to be illustrated.…”

<p>Profiling and Integrated Analysis of Differentially Expressed Circular RNAs in Plasma Exosomes as Novel Biomarkers for Advanced-Stage Lung Adenocarcinoma</p>

“…36 Further, recent research has demonstrated that exosomal circRNAs affect LUAD development and progression by modulating the activation of LUAD-associated molecules and pathways and may be used as LUAD markers for treatment. [22][23][24] GO and KEGG pathway analyses were used to further understand the potential role of the differentially expressed circRNAs. These genes were assessed using GO function analysis to annotate likely functions.…”

Identified GNGT1 and NMU as Combined Diagnosis Biomarker of Non-Small-Cell Lung Cancer Utilizing Bioinformatics and Logistic Regression

“…In an attempt to improve treatments for NSCLC, new therapeutic strategies, such as the development of noncytotoxic targeted agents, have emerged [ 24 – 27 ]. Moreover, the targeted therapies have significantly improved clinical outcomes in a subset of lung cancer patients whose tumors harbor EGFR [ 28 ], ALK [ 29 , 30 ], and HER2 alterations [ 31 – 33 ].…”