circRNA‐associated ceRNA network construction reveals the circRNAs involved in the progression and prognosis of breast cancer

Ma, Xiaoran; Liu, Cun; Gao, Chundi; Li, Jie; Zhuang, Jing; Liu, Lijuan; Li, Huayao; Wang, Xue; Zhang, Xiaoming; Dong, Shixia; Zhou, Chao; Sun, Changgang

doi:10.1002/jcp.29291

Cited by 32 publications

(20 citation statements)

References 45 publications

(41 reference statements)

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“…Emerging evidence shows that circRNAs are tightly associated with the occurrence of malignant tumors, and their dysfunction could inhibit tumor growth and metastasis. 16,17 For example, Huang et al 18 discovered that circRNA_100876 was dysregulated in esophageal squamous cell carcinoma, and that its expression was closely linked to clinical progression, suggesting that it could be a promising prognostic biomarker. These studies, together with the current results, suggest that circRNA_100395 may be a potential therapeutic biomarker and target in prostate cancer.…”

Section: Discussionmentioning

confidence: 99%

Inhibitory role of circRNA_100395 in the proliferation and metastasis of prostate cancer cells

Luo

et al. 2021

J Int Med Res

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Objective Circular RNAs (circRNAs) are non-coding RNAs with high cancer-specific expression and the potential for regulating tumorigenesis. CircRNA_100395 is expressed at low levels in many cancers and is involved in the regulation of tumor cell proliferation and metastasis. However, its expression and function in prostate cancer remain unclear. Methods Endogenous expression levels of circRNA_100395 and microRNA-1228 (miR-1228) in prostate cancer tissue samples and cell lines were detected by quantitative reverse transcription-polymerase chain reaction. Cell proliferation, invasion, and migration, cell cycle distribution, and epithelial–mesenchymal transition (EMT) were analyzed in circRNA_100395-overexpressing prostate cancer cells by Cell Counting Kit-8, flow cytometry, Transwell assay, and western blotting, respectively. Results CircRNA_100395 expression was downregulated in cancerous prostate tissues relative to adjacent normal tissues. CircRNA_100395 expression was negatively correlated with tumor size, Gleason score, tumor stage, and lymph node metastasis. Moreover, circRNA_100395 overexpression inhibited cell proliferation, altered cell cycle distribution, reduced cell migration and invasion abilities, and suppressed EMT in prostate cancer cells. Moreover, miR-1228 was a direct downstream target of circRNA_100395, and the anti-tumor ability of circRNA_100395 was significantly reversed by miR-1228. Conclusion This study identified circRNA_100395 as an anti-tumor circRNA and a potential therapeutic target for prostate cancer.

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Section: Discussionmentioning

confidence: 99%

Inhibitory role of circRNA_100395 in the proliferation and metastasis of prostate cancer cells

Luo

et al. 2021

J Int Med Res

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“…Accumulating evidence indicates that circRNAs play a vital role in modulating tumor development by maintaining cellular homeostasis. 16 The involvement of circRNAs in different types of cancers has also been reported. 17 They can play a role of regulatory interaction with miRNA to prevent mRNA translation, bind to RNA-associated proteins or influence gene expression by regulating gene splicing or mRNA levels.…”

Section: Introductionmentioning

confidence: 99%

Gut microbiota regulate tumor metastasis via circRNA/miRNA networks

Zhu

Huang

et al. 2020

Gut Microbes

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Background: Increasing evidence indicates that gut microbiota plays an important role in cancer progression. However, the underlying mechanism remains largely unknown. Here, we report that broad-spectrum antibiotics (ABX) treatment leads to enhanced metastasis by the alteration of gut microbiome composition. Methods: Cancer LLC and B16-F10 cell metastasis mouse models, and microarray/RNA sequencing analysis were used to reveal the regulatory functions of microbiota-mediated circular RNA (circRNA)/microRNA (miRNA) networks that may contribute to cancer metastasis. Results: The specific pathogen-free (SPF) mice with ABX treatment demonstrated enhanced lung metastasis. Fecal microbiota transplantation (FMT) from SPF mice or Bifidobacterium into germ-free mice significantly suppressed lung metastasis. Mechanistically, gut microbiota impacts circRNA expression to regulate levels of corresponding miRNAs. Specifically, such modulations of gut microbiota inhibit mmu_circ_0000730 expression in an IL-11-dependent manner. Bioinformatics analysis combined with luciferase reporter assays revealed reciprocal repression between mmu_-circ_0000730 and mmu-miR-466i-3p. We further showed that both mmu-miR-466i-3p and mmu-miR-466 f-3p suppresses a number of genes involved in epithelial-mesenchymal transition (EMT) and stemness of cancer stem cells such as SOX9. Conclusions: These results provide evidence of a previously unrecognized regulatory role of noncoding RNAs in microbiota-mediated cancer metastasis, and thus, the microbiome may serve as a therapeutic target.

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“…Circular RNA (circRNA) is a non-coding RNA with a special circular structure and high stability in eukaryotic cells (Li et al, 2018;Patop et al, 2019). Researchers have confirmed that circRNA can be used as a competitive endogenous RNA (ceRNA) for microRNA (miRNA) to regulate the expression of downstream targets (Ma et al, 2020). Studies have found that circRNA is closely related to the progression of a variety of cardiovascular diseases, including AMI (Altesha et al, 2019;Gong et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Circ_0124644 Serves as a ceRNA for miR-590-3p to Promote Hypoxia-Induced Cardiomyocytes Injury via Regulating SOX4

et al. 2021

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Circular RNA (circRNA) is an important factor for regulating the progression of many cardiovascular diseases, including acute myocardial infarction (AMI). However, the role of circ_0124644 in AMI progression remains unclear. Hypoxia was used to induce cardiomyocytes injury. The expression of circ_0124644, microRNA (miR)-590-3p, and SRY-box transcription factor 4 (SOX4) mRNA was measured by qRT-PCR. Cell counting kit 8 (CCK8) assay and flow cytometry were utilized to detect cell viability, cell cycle progression, and apoptosis. The protein levels of apoptosis markers and SOX4 were determined by western blot (WB) analysis, and the levels of oxidative stress markers were assessed using commercial Assay Kits. Dual-luciferase reporter assay, RIP assay, and RNA pull-down assay were employed to confirm the interaction between miR-590-3p and circ_0124644 or SOX4. Circ_0124644 was upregulated in AMI patients and hypoxia-induced cardiomyocytes. Hypoxia could inhibit cardiomyocytes viability, cell cycle process, and promote apoptosis and oxidative stress, while silencing circ_0124644 could alleviate hypoxia-induced cardiomyocytes injury. In terms of mechanism, circ_0124644 could target miR-590-3p. MiR-590-3p overexpression could relieve hypoxia-induced cardiomyocytes injury. Also, the suppressive effect of circ_0124644 knockdown on hypoxia-induced cardiomyocytes injury could be reversed by miR-590-3p inhibitor. Moreover, SOX4 was found to be a target of miR-590-3p, and its overexpression also could reverse the regulation of miR-590-3p on hypoxia-induced cardiomyocytes injury. Circ_0124644 silencing could alleviate hypoxia-induced cardiomyocytes injury by regulating the miR-590-3p/SOX4 axis, suggesting that it might be a target for alleviating AMI.

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circRNA‐associated ceRNA network construction reveals the circRNAs involved in the progression and prognosis of breast cancer

Cited by 32 publications

References 45 publications

Inhibitory role of circRNA_100395 in the proliferation and metastasis of prostate cancer cells

Inhibitory role of circRNA_100395 in the proliferation and metastasis of prostate cancer cells

Gut microbiota regulate tumor metastasis via circRNA/miRNA networks

Circ_0124644 Serves as a ceRNA for miR-590-3p to Promote Hypoxia-Induced Cardiomyocytes Injury via Regulating SOX4

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