Circadian dynamics of the teleost skin immune-microbiome interface

Ellison, Amy; Wilcockson, David; Cable, Joanne

doi:10.1101/2021.01.29.428758

Cited by 1 publication

(1 citation statement)

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“…Additionally, fish age has been seen to influence individual taxa abundances but offers a limited explanation of inter-individual variation at the microbial community level (Rosado et al, 2021). Furthermore, the time of day of sampling has a large influence on fish skin microbiota (Ellison et al, 2021). Longitudinal studies that monitor the microbiomes of individual tilapia in aquaculture ponds over time would provide valuable insight into their dynamics and help inform on the variability on responses to the physicochemical fluctuations in these environments.…”

Section: Discussionmentioning

confidence: 99%

Relationships between pond water and tilapia skin microbiomes in aquaculture ponds in Malawi

McMurtrie

Alathari

Chaput

et al. 2021

Preprint

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Intensification of fish farming practices is being driven by the demand for increased food production to support a rapidly growing global human population, particularly in lower-middle income countries. Intensification of production, however, increases the risk of disease outbreaks and thus likelihood for crop losses. The microbial communities that colonise the skin mucosal surface of fish are poorly understood, but are important in maintaining fish health and resistance against disease. This skin microbial community is susceptible to disruption through stressors associated with transport, handling and the environment of intensive practices, and this risks the propagation of disease-causing pathogens. In this study, we characterised the microbial assemblages found on tilapia skin - the most widely farmed finfish globally - and in the surrounding water of seven earthen aquaculture ponds from two pond systems in distinct geographic regions in Malawi. Metabarcoding approaches were used to sequence the prokaryotic and microeukaryotic communities. We found 92% of prokaryotic amplicon sequence variants were common to both skin and water samples. Differentially enriched and core taxa, however, differed between the skin and water samples. In tilapia skin, Cetobacterium, Paucibacter, Pseudomonas and Comamonadaceae were enriched, whereas, the cyanobacteria Cyanobium, Microcystis and/or Synechocystis, and the diatom Cyclotella, were most prevalent in pond water. Ponds that clustered together according to their water prokaryotic communities also had similar microeukaryotic communities indicating strong environmental influences on prokaryotic and microeukaryotic community structures. While strong site-specific clustering was observed in pond water, the grouping of tilapia skin prokaryotes by pond site was less distinct, suggesting fish microbiota have a greater buffering capacity against environmental influences. The characterised diversity, structure and variance of microbial communities associated with tilapia culture in Malawi provides the baseline for studies on how future intensification practices may lead to microbial dysbiosis and disease onset.

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Section: Discussionmentioning

confidence: 99%