circ_PTN contributes to -cisplatin resistance in glioblastoma via PI3K/AKT signaling through the miR-542-3p/PIK3R3 pathway

Luo, Hongcheng; Yi, Tingzhuang; Huang, Deyou; Chen, Xiaoping; Xu, Liping; Wan, Qianquan; Huang, Haiyang; Huang, Hao; Wei, Hongyu; Song, Ye; Que, Tianshi; Hu, Rentong; Huang, Hua‐Tuo; Luo, Kunxiang; Li, Chuanyu; Qin, Chengjian; Zheng, Chuanhua; Lan, Chuanliu; Chen, Wen‐Cheng; Zhou, Dan; Luo, Qiong

doi:10.1016/j.omtn.2021.08.034

Cited by 18 publications

(14 citation statements)

References 39 publications

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“…Previous studies have shown that the PI3K-AKT pathway plays an important role in DDP resistance [ 29–32 ]. We speculate dwhether PTPRZ1 was involved in DDP resistance of OC by regulating the PI3K-AKT pathway.…”

Section: Discussionmentioning

confidence: 99%

RETRACTED ARTICLE: Protein tyrosine phosphatase receptor type Z1 inhibits the cisplatin resistance of ovarian cancer by regulating PI3K/AKT/mTOR signal pathway

Wang

et al. 2022

Bioengineered

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Most patients with ovarian cancer (OC) get remission after undergoing cytoreductive surgery and platinum-based standard chemotherapy, but more than 50% of patients with advanced OC relapse within the first 5 years after treatment and develop resistance to standard chemotherapy. The production of medicinal properties is the main reason for the poor prognosis and high mortality of OC patients. Cisplatin (DDP) resistance is a major cause for poor prognosis of OC patients. PTPRZ1 can regulate the growth and apoptosis of ovarian cancer cells, while the molecular mechanism remains unknown. This study was designed to investigate the roles of PTPRZ1 in DDP-resistant OC cells and possible mechanism. PTPRZ1 expression in OC tissues and normal tissues was analyzed by GEPIA database and verified by Real-time Quantitative Reverse Transcription PCR (RT-PCR) assay. PTPRZ1 expression in normal ovarian cancer cells and DDP-resistant OC cells was also analyzed. Subsequently, RT-PCR, Western blot, MTT experiment and flow cytometry were used to assess the effects of PTPRZ1-PI3K/AKT/mTOR regulating axis on DDP resistance of OC. PTPRZ1 expression was abnormally low in OC tissues, and notably reduced in DDP-resistant OC cells. MTT experiment and flow cytometer indicated that overexpression of PTPRZ1 enhanced the DDP sensitivity of OC cells and promoted the cell apoptosis. Moreover, the results of our research showed that PTPRZ1 might exert its biological effects through blocking PI3K/AKT/mTOR pathway. PTPRZ1 overexpression inhibitied OC tumor growth and resistance to DDP in vivo. Overall, PTPRZ1 might suppress the DDP resistance of OC and induce the cytotoxicity by blocking PI3K/AKT/mTOR pathway.

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Section: Discussionmentioning

confidence: 99%

RETRACTED ARTICLE: Protein tyrosine phosphatase receptor type Z1 inhibits the cisplatin resistance of ovarian cancer by regulating PI3K/AKT/mTOR signal pathway

Wang

et al. 2022

Bioengineered

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“…3a, the Mo/AlN/Mo film stacks are sputtered on a silicon-on-insulator (SOI) wafer. AlN film, deposited at 300 °C by DC-pulse reactive magnetron sputtering, achieves average stress within +/− 50 MPa and a stress range within 200 MPa based on our previous work 46,47 . The top patterned Mo electrode is typically designed at a size of 65-70% of the cavity diameter in order to effectively collect the charge of AlN piezoelectric layer.…”

Section: Pmut Device and Systemmentioning

confidence: 75%

Beyond fundamental resonance mode: high-order multi-band ALN PMUT for in vivo photoacoustic imaging

et al. 2022

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This paper reports on an aluminum nitride (AlN) piezoelectric micromachined ultrasound transducer (PMUT) array for photoacoustic (PA) imaging, where the high-order resonance modes of the PMUT are utilized to improve imaging resolution. A flexural vibration mode (FVM) PMUT is fabricated and applied in a photoacoustic imaging (PAI) system. Specifically, the microelectromechanical system (MEMS)-based PMUT is suitable for PA endoscopic imaging of blood vessels and bronchi due to its miniature size and high sensitivity. More importantly, AlN is a nontoxic material, which makes it harmless for biomedical applications. In the PAI system, the AlN PMUT array is used to detect PA signals, and the acousto–mechanical response is designed and optimized at the PMUT’s fundamental resonance. In this work, we focus on the high-order resonance performance of the PMUT PAI beyond the fundamental resonance. The acoustic and electrical responses of the PMUT’s high-order resonance modes are characterized and analyzed. The fundamental and three high-order resonance bandwidths are 2.2, 8.8, 18.5, and 48.2 kHz. Compared with the resolution at the fundamental resonance mode, the resolutions at third- and fourth-order resonance modes increase by 38.7% and 76.9% in a phantom experiment. The high-order resonance modes of the AlN PMUT sensor array provide higher central frequency and wider bandwidth for PA signal detection, which increase the resolution of PAI compared to the PMUT working at the fundamental resonance mode.

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“…Recently, circRNAs have become more attention as new prognostic or therapeutic targets for tumors. CircRNAs were found to be differentially expressed in GBM [25][26][27][28][29][30][31][32]. In addition, previous research showed that circRNA was regulated by HIF-1α under hypoxia condition.…”

Section: Discussionmentioning

confidence: 99%

Profiling and Bioinformatics Analyses of Differential Circular RNA Expression in Glioblastoma Multiforme Cells Under Hypoxia

Chen

Yang

et al. 2022

J Mol Neurosci

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BackgroundThe hypoxia microenvironment is highly associated with GBM's malignant phenotypes. CircRNAs were reported involved in GBM's biological characteristics and regulated by HIF-1α. However, the differential expression pro le and role of circRNAs in GBM cells under hypoxia are still unclear. MethodsThe expression pro les of circRNAs in LN229 and T98G under hypoxia were explored via circRNA sequencing analysis. Those circRNAs signi cantly dysregulated both in LN229 and T98G, and could be found in circBase were selected and validated by qRT-PCR, RNase R digestion reaction and Sanger sequencing. Normal cell line and fresh GBM tissues were also used for qRT-PCR validation. The roles of differentially expressed circRNAs were evaluated by bioinformatics analyses. ResultsThere were 672 dysregulated circRNAs in LN229 and 698 dysregulated circRNAs in T98G. GO analysis indicated that the alteration of circRNAs expression related to GBM cell's biogenesis and metabolism. KEGG analysis demonstrated that TGF-β signaling pathway, HIF-1 signaling pathway and metabolism related signaling pathway were closely associated with differentially expressed circRNAs under hypoxia.These results were con rmed by GSEA analysis. The 6 selected and dysregulated circRNAs both in LN229 and T98G including hsa_circ_0000745, hsa_circ_0020093, hsa_circ_0020094, hsa_circ_0000943, hsa_circ_0004874 and hsa_circ_0002359 were validated by qRT-PCR. Inhibition of hsa_circ_0000745 inhibited GBM cell's proliferation, migration and invasion. HIF-1α centered circRNA-miRNA-mRNA networks analysis showed that the 6 validated circRNAs could cross-talk with 11 related miRNAs. ConclusionThe circRNA expressions are dysregulated in GBM cell under hypoxia. The 6 validated circRNAs could participate in GBM's development and progression when hypoxia occurs. They might be the candidates for prognostic markers and adjuvant therapeutics of GBM in the future.

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circ_PTN contributes to -cisplatin resistance in glioblastoma via PI3K/AKT signaling through the miR-542-3p/PIK3R3 pathway

Cited by 18 publications

References 39 publications

RETRACTED ARTICLE: Protein tyrosine phosphatase receptor type Z1 inhibits the cisplatin resistance of ovarian cancer by regulating PI3K/AKT/mTOR signal pathway

RETRACTED ARTICLE: Protein tyrosine phosphatase receptor type Z1 inhibits the cisplatin resistance of ovarian cancer by regulating PI3K/AKT/mTOR signal pathway

Beyond fundamental resonance mode: high-order multi-band ALN PMUT for in vivo photoacoustic imaging

Profiling and Bioinformatics Analyses of Differential Circular RNA Expression in Glioblastoma Multiforme Cells Under Hypoxia

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