Circ_0026134 promotes <scp>NSCLC</scp> progression by the <scp>miR</scp>‐3619‐5p/<scp>CHAF1B</scp> axis

Ge, Liang; Tan, Wei; Li, Guangcai; Gong, Nianjin; Zhou, Long

doi:10.1111/1759-7714.14301

Cited by 4 publications

(2 citation statements)

References 31 publications

(41 reference statements)

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“…Multiple circRNAs presents stable and abnormal expression in NSCLC, with good sensitivity and specificity, and has been illustrated to have relation to unfavourable prognosis, 19–21 providing a new choice for early diagnosis and development of prognostic markers for NSCLC. CircDHTKD1 have been revealed as an oncogene to facilitate metastasis in bladder cancer as well as oral squamous cell carcinoma 22,23 .…”

Section: Discussionmentioning

confidence: 99%

EIF4A3‐Induced CircDHTKD1 regulates glycolysis in non‐small cell lung cancer via stabilizing PFKL

Liu,

Li,

Wang

et al. 2024

J Cellular Molecular Medi

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Lung cancer (LC) is one of the malignancies with the highest incidence and mortality in the world, approximately 85% of which is non‐small cell lung cancer (NSCLC). Circular RNAs (circRNAs) exert multiple roles in NSCLC occurrence and development. The sequencing results in previous literature have illustrated that multiple circRNAs exhibit upregulation in NSCLC. We attempted to figure out which circRNA exerts an oncogenic role in NSLCL progression. RT‐qPCR evaluated circDHTKD1 level in NSCLC tissue specimens and cells. Reverse transcription as well as RNase R digestion assay evaluated circDHTKD1 circular characterization in NSCLC cells. FISH determined circDHTKD1 subcellular distribution in NSCLC cells. Loss‐ and gain‐of‐function assays clarified circDHTKD1 role in NSCLC cell growth, tumour growth and glycolysis. Bioinformatics and RIP and RNA pull‐down assessed association of circDHTKD1 with upstream molecule Eukaryotic initiation factor 4A‐III (EIF4A3) or downstream molecule phosphofructokinase‐1 liver type (PFKL) and insulin‐like growth factor 2 mRNA binding protein 2 (IGF2BP2) in NSCLC cells. Rescue assays assessed regulatory function of PFKL in circDHTKD1‐meidated NSCLC cellular phenotypes. CircDHTKD1 exhibited upregulation and stable circular nature in NSCLC cells. EIF4A3 upregulated circDHTKD1 in NSCLC cells. CircDHTKD1 exerted a promoting influence on NSCLC cell malignant phenotypes and tumour growth. CircDHTKD1 exerted a promoting influence on NSCLC glucose metabolism. CircDHTKD1 exerts a promoting influence on NSCLC glucose metabolism through PFKL upregulation. RIP and RNA pull‐down showed that circDHTKD1 could bind to IGF2BP, PFKL could bind to IGF2BP2, and circDHTKD1 promoted the binding of PFKL to IGF2BP2. In addition, RT‐qPCR showed that IGF2BP2 knockdown promoted PFKL mRNA degradation, suggesting that IGF2BP2 stabilized PFKL in NSCLC cells. CircDHTKD1 exhibits upregulation in NSCLC. We innovatively validate that EIF4A3‐triggered circDHTKD1 upregulation facilitates NSCLC glycolysis through recruiting m6A reader IGF2BP2 to stabilize PFKL, which may provide a new direction for seeking targeted therapy plans of NSCLC.

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Section: Discussionmentioning

confidence: 99%

EIF4A3‐Induced CircDHTKD1 regulates glycolysis in non‐small cell lung cancer via stabilizing PFKL

Liu,

Li,

Wang

et al. 2024

J Cellular Molecular Medi

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“…At present, the function of circRNAs in cancer progression has attracted increasing attention, and researchers are seeking to develop effective drugs for the target treatment of cancer ( 7 ). Famously, circRNAs can participate in the post-transcriptional regulation of coding gene expression in cancer by acting as microRNA (miRNA) sponges ( 8 ). The significance of circRNAs in the pathogenesis of NSCLC has been established ( 9 , 10 ).…”

Section: Introductionmentioning

confidence: 99%

Circ_0087378 intensifies the malignant behavior of non-small cell lung cancer cells in vitro by facilitating DDR1 via sponging miR-199a-5p

Ming¹,

Li²,

Yi³

et al. 2023

Transl Lung Cancer Res

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Background Circular RNA hsa_circ_0087378 (circ_0087378) has been found to have different functions in different cancer types. However, its function in non-small cell lung cancer (NSCLC) remains unclear. This study revealed the effect of circ_0087378 on the malignant behavior of NSCLC cells in vitro to broaden the options for NSCLC treatment. Methods This study detected the expression of circ_0087378 in NSCLC cells via real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR). The discoidin domain receptor 1 (DDR1) protein in NSCLC cells was investigated through western blot. The influence of circ_0087378 on the malignant behavior of NSCLC cells in vitro was investigated by cell counting kit-8 assay, colony formation assay, Transwell assay, and flow cytometry. Dual-luciferase reporter gene assay and RNA pull-down assay were performed to verify the binding between two genes. Results Circ_0087378 was abundantly expressed in NSCLC cells. The loss of circ_0087378 repressed the proliferation, colony formation, migration, invasion, but enhanced the apoptosis in NSCLC cells in vitro . Circ_0087378 could repress microRNA-199a-5p (miR-199a-5p) by acting as a sponge. The loss of miR-199a-5p abrogated the inhibition of circ_0087378 loss on the malignant phenotype of NSCLC cells in vitro . DDR1 was directly repressed via miR-199a-5p. DDR1 counteracted the repressive role of miR-199a-5p on the malignant behavior of NSCLC cells in vitro . Conclusions Circ_0087378 promotes the malignant behavior of NSCLC cells in vitro by facilitating DDR1 via sponging miR-199a-5p. It may be a promising target for treatment.

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Glycolysis in human cancers: Emphasis circRNA/glycolysis axis and nanoparticles in glycolysis regulation in cancer therapy

Alkhathami

Sahib²,

Fayi

et al. 2023

Environmental Research

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Circ_0026134 promotes NSCLC progression by the miR‐3619‐5p/CHAF1B axis

Cited by 4 publications

References 31 publications

EIF4A3‐Induced CircDHTKD1 regulates glycolysis in non‐small cell lung cancer via stabilizing PFKL

EIF4A3‐Induced CircDHTKD1 regulates glycolysis in non‐small cell lung cancer via stabilizing PFKL

Circ_0087378 intensifies the malignant behavior of non-small cell lung cancer cells in vitro by facilitating DDR1 via sponging miR-199a-5p

Glycolysis in human cancers: Emphasis circRNA/glycolysis axis and nanoparticles in glycolysis regulation in cancer therapy

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