Circ_0000105 promotes liver cancer by regulating miR‐498/PIK3R1

Abstract

Background: A growing number of studies demonstrate that circular RNA (circRNA) has a prominent role in the regulation of numerous biological behaviors of tumor cells. The present study aimed to investigate the expression, function and molecular mechanisms of circ_0000105 in liver cancer. Methods: A quantitative real-time polymerase chain reaction (qRT-PCR) was utilized to obtain the expressions of circ_0000105 and miR-498 in liver cancer tissues and cells. The association between the expression level of circ_0000105 and the clinicopathological characteristics of liver cancer patients was analyzed. qRT-PCR and western blotting were utilized to investigate the expression of phosphoinositide-3-kinase regulatory subunit 1 (PIK3R1) in cells. Cell counting kit-8, bromodeoxyuridine and flow cytometry assays were utilized to determine liver cancer cell proliferation and apoptosis, respectively. Bioinformatics, a luciferase reporter gene experiment and a RNA immunoprecipitation experiment were conducted to predict and confirm the targeting correlation of circ_0000105 and miR-498, as well as miR-498 and PIK3R1. Results: Circ_0000105 was highly expressed in liver cancer tissues and cell lines. Moreover, its high expression was associated with an increase in the T stage of liver cancer patients and a low degree of tumor differentiation. Circ_0000105 overexpression promoted liver cancer cell proliferation and inhibited apoptosis, whereas knocking down circ_0000105 triggered the opposite effect. miR-498 was a downstream target of circ_0000105, inhibiting liver cancer cell proliferation and promoting apoptosis. Mechanistically, circ_0000105 indirectly up-regulated the expression of PIK3R1 by adsorbing miR-498. Conclusions: Circ_0000105 plays a cancer-promoting role in liver cancer by regulating the miR-498/PIK3R1 axis.

ANXA10 is a prognostic biomarker and suppressor of hepatocellular carcinoma: a bioinformatics analysis and experimental validation

“…The etiology of numerous prevalent malignancies is associated with competing endogenous RNAs (ceRNAs), such as endometrial cancer, liver cancer, lung cancer, breast cancer, gastric cancer and prostate cancer 61 . Sun et al found that circ-0000105 indirectly increased PIK3R1 expression by binding miR-498, thereby promoting the proliferation of hepatoma cells 62 . In our study, results from three databases revealed that ANXA10 has 2 potential upstream miRNAs, but only hsa-miR-758-3p was inversely correlated with ANXA10 expression.…”

Circular RNA circ-LARP1B contributes to cutaneous squamous cell carcinoma progression by targeting microRNA-515-5p/TPX2 microtubule nucleation factor axis

“…Sun et al . demonstrated that circ_0000105 facilitates the progression of liver cancer by binding to miR-498 to induce PIK3R1 expression [ 36 ]. CircRNAs have also shown important regulatory role in CSCC progression [ 37 , 38 ].…”

Circular RNA as a Novel Biomarker and Therapeutic Target for HCC

“…Circ_ 0000105 can competitively bind miR-498 to promote the expression of PIK3R1. Circ_0000105 played an important role in tumorigenesis and development [ 34 ]. Wang et al found that circ_10156 may act as a molecular sponge of miR-149-3p.…”