Ciprofibrate quantification in human plasma by high-performance liquid chromatography coupled with electrospray tandem mass spectrometry for pharmacokinetic studies

Mendes, Fabiana D.; Chen, Lu Shi; Borges, André; Babadópulos, Tainah; Ilha, Jaime O.; Alkharfy, Khalid M.; Mendes, Gustavo Duarte; Nucci, Gilberto De

doi:10.1016/j.jchromb.2011.05.055

Cited by 6 publications

(5 citation statements)

References 6 publications

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“…Plasma concentrations of lidocaine were determined by liquid chromatography tandem mass spectrometry method using electrospray ionization (Mendes et al. ; Abimussi et al. ).…”

Section: Methodsmentioning

confidence: 99%

Cardiovascular effects of a continuous rate infusion of lidocaine in calves anesthetized with xylazine, midazolam, ketamine and isoflurane

Araújo¹,

Dias

Bovino

et al. 2014

Veterinary Anaesthesia and Analgesia

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“…Plasma concentrations of lidocaine were determined by liquid chromatography tandem mass spectrometry method using electrospray ionization (Mendes et al. ; Abimussi et al. ).…”

Section: Methodsmentioning

confidence: 99%

Cardiovascular effects of a continuous rate infusion of lidocaine in calves anesthetized with xylazine, midazolam, ketamine and isoflurane

Araújo¹,

Dias

Bovino

et al. 2014

Veterinary Anaesthesia and Analgesia

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“…Considering that the extraction procedures for HPLC-MS-MS bioanalysis are very simple (protein precipitation is routinely employed), one should ask whether the use of structurally related internal standard is really required or advisable. The method described above validated well without the use of the internal standard, similar to other method [11]. Whether this approach applies to other bioanalytical methods should be further investigated.…”

Section: Citationmentioning

confidence: 75%

Hydroxocobalamin Quantification in Human Plasma by High-Performance Liquid Chromatography Coupled with Electrospray Tandem Mass Spectrometry in a Pharmacokinetic Study

Mendes¹

2013

J Bioequiv Availab

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A rapid, sensitive and specific method for quantifying hydroxocobalamin in human plasma using paracetamol as the internal standard (IS) is described. The analyte and the IS were extracted from plasma by liquid-liquid extraction using an organic solvent (ethanol 100%; -20°C). The extracts were analyzed by high performance liquid chromatography coupled with electrospray tandem mass spectrometry (HPLC-MS-MS). Chromatography was performed on Prevail C8 3 μm, analytical column (2.1×100 mm i.d.). The method had a chromatographic run time of 3.4 min and a linear calibration curve over the range 5-400 ng.mL -1 (r>0.9983). The limit of quantification was 5 ng.mL -1 . The method was also validated without the use of the internal standard. The precision in the intra-batch validation with IS was 9.6%, 8.9%, 1.0% and 2.8% whereas without IS was 9.2%, 8.2%, 1.8% and 1.5% for 5, 15, 80 and 320 ng/mL, respectively. The accuracy in intra-batch validation with IS was 108.9%, 99.9%, 98.9% and 99.0% whereas without IS was 101.1%, 99.3%, 97.5% and 92.5% for 5, 15, 80 and 320 ng/mL, respectively. The precision in the inter-batch validation with IS was 9.4%, 6.9%, 4.6% and 5.5% whereas without IS was 10.9%, 6.4%, 5.0% and 6.2% for 5, 15, 80 and 320 ng/mL, respectively. The accuracy in inter-batch validation with IS was 101.9%, 104.1%, 103.2% and 99.7% whereas without IS was 94.4%, 101.2%, 101.6% and 96.0% for 5, 15, 80 and 320 ng/ mL, respectively. This HPLC-MS-MS procedure was used to assess the pharmacokinetics of cobalamin following intramuscular injection 5000 μg in healthy volunteers of both sexes (10 males and 10 females). [51.70-66.70]. The following pharmacokinetic parameters were obtained from the hydroxocobalamin plasma concentration vs. time curves: AUC last , T 1/2 , T max , Vd, Cl, C max and C last . The pharmacokinetic parameters were 120 (± 25) ng.mL -1 for C max , 2044 (± 641) ng.hr.mL -1 for AUC last , 8 (± 3.2) ng.mL -1 for C last , 38 (± 15.8) hr for T 1/2 and 2.5 (range 1-6) hr for T max . Female volunteers presented significant (p=0.0136) lower AUC (1706 ± 704) ng.hr.mL -1 ) and larger (p=0.0205) clearance (2.91 ± 1.41 L/hr), as compared to male 2383 ± 343 ng.hr.mL -1 and 1.76 ± 0.23 L/hr, respectively. These pharmacokinetic differences could explain the higher prevalence of vitamin B12 deficiency in female patients. The method described validated well without the use of the internal standard and this approach should be investigated in other HPLC-MS-MS methods.

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“…In practice, analytical methods developed for LC-MS include a few or only one internal standard for all the assayed metabolites [ 29 , 30 ]. Moreover, metabolite quantification using HPLC-MS has been validated without internal standards [ 31 ], although fully labelled cell extracts which incorporate the isotopic internal standard have been highly recommended due to the increment in the precision of the measurements [ 32 ]. Therefore, EasyLCMS has been tested to operate correctly using a ‘generic’ internal standard, and even in the extreme situation of using no internal standard.…”

Section: Discussionmentioning

confidence: 99%

EasyLCMS: an asynchronous web application for the automated quantification of LC-MS data

et al. 2012

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BackgroundDownstream applications in metabolomics, as well as mathematical modelling, require data in a quantitative format, which may also necessitate the automated and simultaneous quantification of numerous metabolites. Although numerous applications have been previously developed for metabolomics data handling, automated calibration and calculation of the concentrations in terms of μmol have not been carried out. Moreover, most of the metabolomics applications are designed for GC-MS, and would not be suitable for LC-MS, since in LC, the deviation in the retention time is not linear, which is not taken into account in these applications. Moreover, only a few are web-based applications, which could improve stand-alone software in terms of compatibility, sharing capabilities and hardware requirements, even though a strong bandwidth is required. Furthermore, none of these incorporate asynchronous communication to allow real-time interaction with pre-processed results.FindingsHere, we present EasyLCMS (http://www.easylcms.es/), a new application for automated quantification which was validated using more than 1000 concentration comparisons in real samples with manual operation. The results showed that only 1% of the quantifications presented a relative error higher than 15%. Using clustering analysis, the metabolites with the highest relative error distributions were identified and studied to solve recurrent mistakes.ConclusionsEasyLCMS is a new web application designed to quantify numerous metabolites, simultaneously integrating LC distortions and asynchronous web technology to present a visual interface with dynamic interaction which allows checking and correction of LC-MS raw data pre-processing results. Moreover, quantified data obtained with EasyLCMS are fully compatible with numerous downstream applications, as well as for mathematical modelling in the systems biology field.

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Ciprofibrate quantification in human plasma by high-performance liquid chromatography coupled with electrospray tandem mass spectrometry for pharmacokinetic studies

Cited by 6 publications

References 6 publications

Cardiovascular effects of a continuous rate infusion of lidocaine in calves anesthetized with xylazine, midazolam, ketamine and isoflurane

Cardiovascular effects of a continuous rate infusion of lidocaine in calves anesthetized with xylazine, midazolam, ketamine and isoflurane

Hydroxocobalamin Quantification in Human Plasma by High-Performance Liquid Chromatography Coupled with Electrospray Tandem Mass Spectrometry in a Pharmacokinetic Study

EasyLCMS: an asynchronous web application for the automated quantification of LC-MS data

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