Chrysin and its phosphate ester inhibit cell proliferation and induce apoptosis in Hela cells

Zhang, Ting; Chen, Xiaolan; Qu, Lingbo; Wu, Jiahui; Cui, Ran; Zhao, Yufen

doi:10.1016/j.bmc.2004.09.013

Cited by 125 publications

(76 citation statements)

References 30 publications

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“…Chrysin has been demonstrated to possess cancer chemopreventive activity through inhibition of cell proliferation (10,18), and induce apoptosis in various malignant cancer cells (5,11,(15)(16)(17)(20)(21)(22).…”

Section: Discussionmentioning

confidence: 99%

“…Chrysin was initially identified for its anti-oxidant effects and has been shown to possess cancer chemopreventive and anti-tumor effects (6)(7)(8). It inhibits proliferation and induces apoptosis in a variety of cancer cells in vitro, including cells from the prostate, skin, breast, lung, cervix, thyroid cancer and leukemia (5,(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22). However, to the best of our knowledge, the effects of chrysin on uveal melanoma cells have not been previously studied.…”

Section: Introductionmentioning

confidence: 99%

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Chrysin induces cell apoptosis in human uveal melanoma cells via intrinsic apoptosis

Xue

Chen

et al. 2016

Oncology Letters

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Abstract. Uveal melanoma is the most common intraocular malignant tumor in adults. Chrysin is a flavonoid present in honey, propolis, various plants and herbs. In the present study, the cytotoxic effects of chrysin were investigated on human uveal melanoma cell lines (M17 and SP6.5) and associated signaling pathways, and a comparison to the effects on normal ocular cells [scleral fibroblasts and retinal pigment epithelial (RPE) cells] was performed. The effects of chrysin on cell viability were assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Cell apoptosis was determined by using terminal deoxynucleotidyl transferase mediated dUTP nick end-labeling assay. Mitochondrial permeability was determined by JC-1 fluorescein analysis. Cytosol cytochrome c levels, and the activities of caspase-3, -8 and -9 were measured by enzyme-linked immunosorbent assay or colorimetric assay. Chrysin reduced the viability of cultured human melanoma cells in a dose-dependent manner (0, 10, 30 and 100 µM) with IC 50 at 28.3 and 35.8 µM in SP6.5 and M17 cell lines, respectively. Chrysin at 30-100 µM levels selectively reduced the viability of melanoma cells without affecting the viability of scleral fibroblasts and RPE cells. Chrysin increased mitochondrial permeability, the levels of cytosol cytochrome c, and caspase-9 and -3 activities, but not capase-8 activity in uveal melanoma cells. The results of the present study indicate that chrysin induces apoptosis of human uveal melanoma cells via the mitochondrial signaling pathway and suggest that chrysin may be a promising agent in the treatment of uveal melanoma.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Chrysin induces cell apoptosis in human uveal melanoma cells via intrinsic apoptosis

Xue

Chen

et al. 2016

Oncology Letters

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“…Chrysin, the most abundant Free-B-Ring flavonoid in honey, was found to potentiate the antiproliferative effect of various chemotherapeutic agents (14). Furthermore, chrysin was found to be capable of inhibiting cell proliferation and inducing apoptosis in human cervical carcinoma (15), leukemia (16), esophageal squamous cell carcinoma (17), malignant glioma, breast carcinoma (18) and prostate cancer cell lines (19). To extend our knowledge of the antineoplastic role of chrysin, the present study demonstrated the ability of chrysin to induce colon cancer cell death more efficiently compared to 5-FU.…”

Section: Discussionmentioning

confidence: 99%

Free-B-Ring flavonoids as potential lead compounds for colon cancer therapy

Ibrahim

Sobeh

Ismail

et al. 2014

Molecular and Clinical Oncology

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Abstract. Although flavonoids have been identified as a versatile source of anticancer agents, to the best of our knowledge, no study has yet investigated their anticolon cancer activity in depth. Therefore, the aim of this study was to investigate the association between the structural characteristics of flavonoids and their anticolon cancer activity in the Caco-2 human colon cancer cell line. Our findings demonstrated that the hydroxylation of C5 and C7 in ring A significantly enhanced the anticolon cancer activity of flavonoids over that of 5-fluorouracil, the classic reference cytotoxic agent. By contrast, the glycosylation or the presence of hydroxyl groups in ring B significantly decreased flavonoid anticancer activity. Collectively, these findings suggest a novel, rational design of flavonoid-related compounds that may improve the treatment of colorectal cancer.

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“…Chrysin also possesses anti-inflammatory activity related to the the pro-inflammatory activities of COX-2 (24). It has been found to possess cancer chemopreventive properties through inhibiting malignant cell growth, as well as inducing cell death and perturbing cell cycle progression (25)(26)(27).…”

Section: Introductionmentioning

confidence: 99%

Acacia honey and chrysin reduce proliferation of melanoma cells through alterations in cell cycle progression

Canini¹

2010

Int J Oncol

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Abstract. Honey has long been used in medicine for different purposes. Only recently, however, its antioxidant property and preventive effects against different diseases, such as cancer, have been highlighted. Chrysin (5,7-dihydroxyflavone) is a natural flavone commonly found in acacia honey. It has previously been shown to be an anti-tumor agent. In this study, we investigated the antiproliferative role of honey or chrysin on human (A375) and murine (B16-F1) melanoma cell lines. The results of the 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay and the trypan blue exclusion test showed that both the tested compounds were able to induce an antiproliferative effect on melanoma cells in a dose-and time-dependent manner. Flow cytometry analysis indicated that cytotoxicity induced by honey or chrysin was mediated by G 0 /G 1 cell cycle arrest and induction of hyperploid progression. Our results suggest that the anti-proliferative effects of honey are due mainly to the presence of chrysin. Chrysin may therefore be considered a potential candidate for both cancer prevention and treatment. Further investigation is needed to validate the contribution of chrysin in tumor therapy in vivo.

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Chrysin and its phosphate ester inhibit cell proliferation and induce apoptosis in Hela cells

Cited by 125 publications

References 30 publications

Chrysin induces cell apoptosis in human uveal melanoma cells via intrinsic apoptosis

Chrysin induces cell apoptosis in human uveal melanoma cells via intrinsic apoptosis

Free-B-Ring flavonoids as potential lead compounds for colon cancer therapy

Acacia honey and chrysin reduce proliferation of melanoma cells through alterations in cell cycle progression

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