Chryseobacterium chaponense sp. nov., isolated from farmed Atlantic salmon (Salmo salar)

Kämpfer, Peter; Fallschissel, Kerstin; Avendaño‐Herrera, Rubén

doi:10.1099/ijs.0.022004-0

Cited by 46 publications

(36 citation statements)

References 32 publications

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“…The only real difference in fatty-acid profiles appeared to be the presence of iso-C 16:0 3-OH, which was not explicitly mentioned for the genus Chryseobacterium (Montero-Calasanz et al, 2014). However, it was reported for a variety of Chryseobacterium species (Hugo et al, 2003; Kämpfer et al, 2003, 2011; Li et al, 2003; Kim et al, 2005; Shen et al, 2005; Young et al, 2005; Park et al, 2006; Behrendt et al, 2007, 2008; Hantsis-Zacharov and Halpern, 2007; Quan et al, 2007; Vaneechoutte et al, 2007; Szoboszlay et al, 2008; Ilardi et al, 2009; Benmalek et al, 2010; Pires et al, 2010; Wu et al, 2013) in concentrations up to 9% and thus does not constitute a real difference either. Additionally, anteiso-C 15:0 as reported for Epilithonimonas is not mentioned in the description of Chryseobacterium but known to occur in the genus from traces to up to 8.2% (Montero-Calasanz et al, 2014).…”

Section: Resultsmentioning

confidence: 96%

Genome-Based Taxonomic Classification of Bacteroidetes

et al. 2016

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The bacterial phylum Bacteroidetes, characterized by a distinct gliding motility, occurs in a broad variety of ecosystems, habitats, life styles, and physiologies. Accordingly, taxonomic classification of the phylum, based on a limited number of features, proved difficult and controversial in the past, for example, when decisions were based on unresolved phylogenetic trees of the 16S rRNA gene sequence. Here we use a large collection of type-strain genomes from Bacteroidetes and closely related phyla for assessing their taxonomy based on the principles of phylogenetic classification and trees inferred from genome-scale data. No significant conflict between 16S rRNA gene and whole-genome phylogenetic analysis is found, whereas many but not all of the involved taxa are supported as monophyletic groups, particularly in the genome-scale trees. Phenotypic and phylogenomic features support the separation of Balneolaceae as new phylum Balneolaeota from Rhodothermaeota and of Saprospiraceae as new class Saprospiria from Chitinophagia. Epilithonimonas is nested within the older genus Chryseobacterium and without significant phenotypic differences; thus merging the two genera is proposed. Similarly, Vitellibacter is proposed to be included in Aequorivita. Flexibacter is confirmed as being heterogeneous and dissected, yielding six distinct genera. Hallella seregens is a later heterotypic synonym of Prevotella dentalis. Compared to values directly calculated from genome sequences, the G+C content mentioned in many species descriptions is too imprecise; moreover, corrected G+C content values have a significantly better fit to the phylogeny. Corresponding emendations of species descriptions are provided where necessary. Whereas most observed conflict with the current classification of Bacteroidetes is already visible in 16S rRNA gene trees, as expected whole-genome phylogenies are much better resolved.

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Section: Resultsmentioning

confidence: 96%

Genome-Based Taxonomic Classification of Bacteroidetes

et al. 2016

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“…T115 was highly similar to C. chaponense based upon genetic analysis, which was further supported using the polyphasic ap proach. However, some phys- iological and biochemical differences existed between strain T115 and the C. cha po nense type strain (Kämpfer et al 2011), which possibly suggests strain divergence or that they are distinct species/ subspecies. Although C. cha po nense was originally recovered from diseased fish (Kämpfer et al 2011), results from our experimental challenges demonstrated that strain T115 did not induce major pathological changes when administered IP to several salmonid species and did not result in mortality in any challenged fish.…”

Section: Discussionmentioning

confidence: 99%

“…have recently been described that are associated with diseased farmed fish worldwide, including C. piscicola (Ilardi et al 2009), C. chaponense (Kämpfer et al 2011), C. tructae (Zamora et al 2012b), C. oncorhynchi (Zamora et al 2012a), and C. viscerum (Zamora et al 2012c). During these outbreaks, external lesions (Kämpfer et al 2011), deep ulcerations (Ilardi et al 2010, and signs of septicemia (Zamora et al 2012a,b) were reported in infected fish. Unfortunately, unraveling the pathogenesis of these novel fish-pathogenic chryseobacteria has received little attention.…”

Section: Resale or Republication Not Permitted Without Written Consenmentioning

confidence: 99%

“…The findings from this study, in conjunction with our recent findings (Loch et al 2013, Loch & Faisal 2014a) and those of Pridgeon et al (2013), definitively demonstrate that a diversity of chryseobacteria are also associated with North Ame - rican fishes. Isolates highly similar to C. piscicola, C. viscerum, and C. cha ponense were identified in Great Lakes fish stocks, each of which have been previously isolated from diseased salmonids in Chile and Finland (Ilardi et al 2009(Ilardi et al , 2010, diseased rainbow trout Oncorhynchus mykiss in Spain (Zamora et al 2012c), and diseased Atlantic salmon in Chile (Kämpfer et al 2011). In all cases, the original isolations of these novel chryseobacteria were from farmed fishes (Ilardi et al 2009, Kämpfer et al 2011, Zamora et al 2012c, possibly indicating that the stressors associated with aquaculture are necessary for chryseobacteriosis to ensue; however, this study shows that C. chaponense-like and C. viscerum-like bacteria are also associated with wild fishes.…”

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confidence: 99%

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Polyphasic characterization reveals the presence of novel fish-associated Chryseobacterium spp. in the Great Lakes of North America

Loch¹,

Faisal

2015

Dis. Aquat. Org.

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Recent reports suggest an emergence of novel Chryseobacterium spp. associated with aquaculture-reared fish worldwide. Herein, we report on multiple Chryseobacterium spp. infecting Great Lakes fishes that are highly similar to previously detected isolates from Europe, Africa, and Asia but have never before been reported in North America. Polyphasic characterization, which included extensive physiological, morphological, and biochemical analyses, fatty acid profiling, and phylogenetic analyses based upon partial 16S rRNA gene sequences, highlighted the diversity of Great Lakes' fish-associated chryseobacteria and also suggested that at least 2 taxa represent potentially novel Chryseobacterium spp. Screening for the ability of representative chryseobacteria to elicit lesions in experimentally challenged fish showed that they induced varying degrees of pathology, some of which were severe and resulted in host death. Median lethal dose (LD 50 ) experiments for the isolate that elicited the most extensive pathology (Chryseobacterium sp. T28) demonstrated that the LD 50 exceeded 4.5 × 10 8 cfu, thereby suggesting its role as a facultative fish-pathogenic bacterium. Histopathological changes in T28-infected fish included epithelial hyperplasia of the secondary lamellae and interlamellar space that resulted in secondary lamellar fusion, monocytic infiltrate, and mucus cell hyperplasia, all of which are consistent with branchitis, along with monocytic myositis, hemorrhage within the muscle, liver, adipose tissue, and ovaries, spongiosis of white matter of the brain, multifocal edema within the granular cell layer of the cerebellar cortex, and renal tubular degeneration and necrosis. The findings of this study underscore the widespread presence of chryseobacteria infecting Great Lakes fish. KEY WORDS: Chryseobacterium · Fish disease · Great Lakes · Bacterial infections · Flavobacterium Resale or republication not permitted without written consent of the publisherDis Aquat Org 113: [113][114][115][116][117][118][119][120][121][122][123][124][125] 2015 taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes (Bernardet et al. 2002).Paralleling the expansion of the genus, an increasing number of Chryseobacterium spp. have recently been described that are associated with diseased farmed fish worldwide, including C. piscicola (Ilardi et al. 2009), C. chaponense (Kämpfer et al. 2011), C. tructae (Zamora et al. 2012b), C. oncorhynchi (Zamora et al. 2012a), and C. viscerum (Zamora et al. 2012c). During these outbreaks, external lesions (Kämpfer et al. 2011), deep ulcerations (Ilardi et al. 2010, and signs of septicemia (Zamora et al. 2012a,b) were reported in infected fish. Unfortunately, unraveling the pathogenesis of these novel fish-pathogenic chryseobacteria has received little attention. Concomitant with the apparent emergence of fish chryseobacteriosis, which is exacerbated by the bacterium's constitutive resistance to a wide spectrum of antibiotics ...

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“…Thus, Chryseobacterium balustinum Chryseobacterium scophtalmum and Chryseobacterium joostei have been isolated from diseased fish [4-6]. More recently, Chryseobacterium piscicola has been reported to produce mortalities in farmed Atlantic salmon ( Salmo salar ) and rainbow trout ( Oncorhynchus mykiss ) in Chile and Finland [7-9], Chryseobacterium arothri was isolated from the kidneys of the pufferfish Arothron hispidus in Hawaii [10] and Chryseobacterium chaponense from diseased farmed Atlantic salmon in Chile [11]. In fact, some Chryseobacterium species are considered potentially emerging pathogens in fish [4].…”

Section: Introductionmentioning

confidence: 99%

First isolation and characterization of Chryseobacterium shigense from rainbow trout

Zamora¹,

Vela

Palacios³

et al. 2012

BMC Vet Res

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BackgroundThere have been an increasing number of infections in fish associated with different species of Chryseobacterium, being considered potentially emerging pathogens. Nevertheless the knowledge of the diversity of species associated with fish disease is partial due to the problems for a correct identification at the species level based exclusively on phenotypic laboratory methods.ResultsChryseobacterium shigense was isolated from the liver, kidney and gills of diseased rainbow trout in different disease episodes that occurred in a fish farm between May 2008 and June 2009. Identity of the isolates was confirmed by 16 S rRNA gene sequencing and phenotypic characterization. Isolates represented a single strain as determined by random amplified polymorphic DNA analysis.ConclusionsThis is the first description of the recovery of C. shigense from clinical specimens in trout, a very different habitat to fresh lactic acid beverage where it was initially isolated.

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Chryseobacterium chaponense sp. nov., isolated from farmed Atlantic salmon (Salmo salar)

Cited by 46 publications

References 32 publications

Genome-Based Taxonomic Classification of Bacteroidetes

Genome-Based Taxonomic Classification of Bacteroidetes

Polyphasic characterization reveals the presence of novel fish-associated Chryseobacterium spp. in the Great Lakes of North America

First isolation and characterization of Chryseobacterium shigense from rainbow trout

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