Chronic perfluorooctanesulfonic acid exposure disrupts lipid metabolism in zebrafish

Cui, Yuan; Lv, Sha; Liu, Jianghuai; Nie, Shangfei; Chen, J; Dong, Qian; Huang, Changjiang; Yang, Dongren

doi:10.1177/0960327116646615

Cited by 56 publications

(30 citation statements)

References 38 publications

(71 reference statements)

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“…However, we did not conduct any related phenotypic analyses. Thus, we were only able to obtain the annotation details of these genes (i.e., dusp22 , ghsra , fgf19 , esm1 , and gpc4 ), which are important for controlling cell division and regulating growth 31 – 34 . Furthermore, bmp10 encodes a potent inhibitor of endothelial cell migration and growth 35 .…”

Section: Discussionmentioning

confidence: 99%

Analyzing homoeolog expression provides insights into the rediploidization event in gynogenetic hybrids of Carassius auratus red var. × Cyprinus carpio

Ren

Cui

Wang

et al. 2017

Sci Rep

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Rediploidization is considered to be a part of the evolutionary history of allotetraploids, and resulted in the emergence of novel epigenetic regulatory activities. To study the changing patterns of gene expression following the reduction of a genome by 50%, we used RNA-seq and quantitative real-time PCR (qPCR) to investigate total gene expression and homoeolog expression in three hybrids of a C. auratus red var. (2n = 100, ♀) (R) and C. carpio (2n = 100, ♂) (C) (i.e., F1, F18, and G4) and their original parents. A comparison of homoeolog expression between G4 and F18 identified 7 genes (0.22%) that exhibited novel R/C homoeolog expression patterns in G4, while 4 genes (0.12%) were affected by R/C homoeolog silencing. We determined the direction and extent of the homoeolog expression bias (HEB). The C-HEB genes (i.e., nrp1a and igf1) and R-HEB genes (i.e., fgf23 and esm1) provided insights into the effects of the dominance of one parental homoeolog expression on growth regulation. This dominance may contribute to the rapid growth of G4 fish. Our findings may be relevant for clarifying the relationship between growth heterosis and differences in homoeolog expression patterns.

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Section: Discussionmentioning

confidence: 99%

Analyzing homoeolog expression provides insights into the rediploidization event in gynogenetic hybrids of Carassius auratus red var. × Cyprinus carpio

Ren

Cui

Wang

et al. 2017

Sci Rep

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show abstract

“…In addition, ICV injections of a MSH (MC4R) receptor agonist (melanotan II) suppress hypothalamic NPY expression (Kojima et al, 2010), and hypothalamic α-MSH-containing neurons are in close contact to NPY-containing nerve fibers, suggesting that the anorexigenic actions of the melanocortin system are mediated in part by an inhibition of the NPY system. In zebrafish larvae, although early ISH studies could not detect fasting-induced changes in hypothalamic POMC transcript levels (Song et al, 2003), more recent qPCR studies indicate that POMCa expression decreases in starved fish (Shanshan et al, 2016).…”

Section: Hormones Involved In Food Intakementioning

confidence: 99%

The Neuroendocrine Regulation of Food Intake in Fish: A Review of Current Knowledge

2016

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Fish are the most diversified group of vertebrates and, although progress has been made in the past years, only relatively few fish species have been examined to date, with regards to the endocrine regulation of feeding in fish. In fish, as in mammals, feeding behavior is ultimately regulated by central effectors within feeding centers of the brain, which receive and process information from endocrine signals from both brain and peripheral tissues. Although basic endocrine mechanisms regulating feeding appear to be conserved among vertebrates, major physiological differences between fish and mammals and the diversity of fish, in particular in regard to feeding habits, digestive tract anatomy and physiology, suggest the existence of fish- and species-specific regulating mechanisms. This review provides an overview of hormones known to regulate food intake in fish, emphasizing on major hormones and the main fish groups studied to date.

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“…Numerous studies have documented homologies between zebrafish and human lipid metabolism [ 23 , 24 , 25 , 26 , 27 ]. In fact, the zebrafish has recently become an important model organism for studying NAFLD [ 28 ].…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-27b Depletion Enhances Endotrophic and Intravascular Lipid Accumulation and Induces Adipocyte Hyperplasia in Zebrafish

Hsu

Lai

Lin

et al. 2017

IJMS

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miR-27b has emerged as a regulatory hub in cholesterol and lipid metabolism, and as a potential therapeutic target for treating atherosclerosis and obesity. However, the impact of miR-27b on lipid levels in vivo remains to be determined. Zebrafish lipids are normally stored as triacylglycerols (TGs) and their main storage sites are visceral, intramuscular, and subcutaneous lipid depots, and not blood vessels and liver. In this study, we applied microRNA-sponge (miR-SP) technology and generated zebrafish expressing transgenic miR-27b-SP (C27bSPs), which disrupted endogenous miR-27b activity and induced intravascular lipid accumulation (hyperlipidemia) and the early onset of nonalcoholic fatty liver disease (NAFLD) and nonalcoholic steatohepatitis (NASH). Oil Red O staining predominantly increased in the blood vessels and livers of larvae and juvenile C27bSPs, indicating that miR-27b depletion functionally promoted lipid accumulation. C27bSPs also showed an increased weight gain with larger fat pads, resulting from adipocyte hyperplasia. Molecular analysis revealed that miR-27b depletion increased the expression of genes that are associated with lipogenesis and the endoplasmic reticulum (ER). Moreover, miR-27b-SP increased peroxisome proliferator-activated receptor γ (PPAR-γ), CCAAT enhancer binding protein-α (C/EBP-α, and sterol regulatory element binding transcription factor 1c (SREBP-1c) expression and contributed to lipogenesis and adipogenesis. Conclusion: Our results suggest that miR-27b-SP acts as a lipid enhancer by directly increasing the expression of several lipogenic/adipogenic transcriptional factors, resulting in increased lipogenesis and adipogenesis. In this study, miR-27b expression improved lipid metabolism in C27bSPs, which suggests that miR-27b is an important lipogenic factor in regulating early onset of hyperlipidemia and adipogenesis in zebrafish.

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Chronic perfluorooctanesulfonic acid exposure disrupts lipid metabolism in zebrafish

Cited by 56 publications

References 38 publications

Analyzing homoeolog expression provides insights into the rediploidization event in gynogenetic hybrids of Carassius auratus red var. × Cyprinus carpio

Analyzing homoeolog expression provides insights into the rediploidization event in gynogenetic hybrids of Carassius auratus red var. × Cyprinus carpio

The Neuroendocrine Regulation of Food Intake in Fish: A Review of Current Knowledge

MicroRNA-27b Depletion Enhances Endotrophic and Intravascular Lipid Accumulation and Induces Adipocyte Hyperplasia in Zebrafish

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