Chronic arsenic exposure and urinary 8-Hydroxy-2′-deoxyguanosine in an arsenic-affected area in Inner Mongolia, China

Abstract

Objective: Recent studies have shown that generation of reactive oxidants during arsenic metabolism can play an important role in arsenic-induced injury. The purpose of this study was to examine the relationship between arsenic in drinking water and oxidative stress in humans by measuring 8-Hydroxy-2 0 -deoxyguanosine (8-OHdG). Methods: We performed a cross-sectional study in an arsenic-affected village in Hetao Plain, Inner Mongolia, China. A total of 134 of the 143 inhabitants (93.7%) of the village participated in the study. The levels of 8-OHdG, arsenic and its metabolites were measured in urine collected from the participants. Regression analyses were performed to investigate the relationship between arsenic species and 8-OHdG levels in urine. Results: In the polluted village, monomethylarsenic was significantly higher in subjects with arsenic dermatosis than those without dermatosis despite no difference in mean levels of arsenic in well water between both types of subject. For subjects with arsenic dermatosis, arsenic species and metabolites in urine are significantly associated with 8-OHdG, while there was no statistically significant relationship for subjects without arsenic dermatosis. For all residents of the polluted village, the levels of dimethylarsenic and 8-OHdG were significantly higher for those who had been exposed to well water for more than 12 years. Conclusions: These results provide evidence of a link between exposure to arsenic from drinking water and oxidative stress, which may play an important role in arsenic-involved injuries.

Low 8-oxo-7,8-dihydro-2′-deoxyguanosine levels and influence of genetic background in an Andean population exposed to high levels of arsenic

“…The 8-OxodG levels detected were low compared to the other studies of As-exposed populations with similar exposure range [8][9][10][11]. It is important to note that the high 8-OxodG levels detected in other studies [8, [10][11][12][13][14][15] are partly due to the methods used; especially ELISA, the most commonly used method for 8oxodG determination, has been shown to overestimate 8-OxodG levels, whereas mass spectrometric techniques appear to be more robust [45].…”

“…As exposure and 8oxodG where they have employed similar LC/MS-MS methods higher 8oxodG levels were found: Fujino et al [9] reported mean 8-OxodG levels of 5.5 μg/g creatinine among subjects without As dermatosis and mean U-As levels of 500 μg/g creatinine; Hu et al [21] reported 8-OxodG levels of 5.6 μg/g creatinine among exposed semiconductor workers with U-As levels of 33 μg/g cre. Thus, the method used in the present study cannot explain the low concentrations of 8-OxodG in this population.…”

“…Associations between 8-OxodG and As have been seen in several studies [7][8][9][10][11][12][13]. However, other studies did not find any association among none-diseased individuals [9,[14][15].…”

Fragmentation of the DNA Repair Enzyme, OGG1, in Mouse Nonparenchymal Liver Cells by Arsenic Compounds

“…For this purpose, understanding the mechanisms of 8-OH-Gua generation is an important issue. As for arsenic compounds, many reports have indicated that they increased oxidative DNA damage in cultured cells (6,7). For example, hydroxyl radical-induced oxidative DNA damage was generated in arsenic trioxide-treated keratinocyes (10).…”

“…In particular, they are associated with the development of several kinds of tumors, including lung, skin, kidney, bladder and liver tumors (1)(2)(3)(4)(5). Recently, arsenic trioxide was shown to induce DNA damage, such as 8-hydroxyguanine (8-OH-Gua) and DNA strand breaks, in cultured human cells (6,7). It was also reported that the urinary 8-OH-dG level is increased with exposure to arsenic compounds (8,9).…”