Chromosome and plasmid-encoded N-acyl homoserine lactones produced by Agrobacterium vitis wildtype and mutants that differ in their interactions with grape and tobacco

Li, Yaxin; Gronquist, Matthew R.; Hao, Ge‐Fei; Holden, Michele R.; Eberhard, Anatol; Scott, Russell; Savka, Michael A.; Szegedi, E.; Süle, S.; Burr, Thomas J.

doi:10.1016/j.pmpp.2006.04.002

Cited by 12 publications

(9 citation statements)

References 33 publications

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“…The long‐chain AHL signal(s) detected from vitopine‐type strains remained near the origin, gave an Rf value of c . 0.06, which is consistent with long‐chain AHL(s) detected with extracts from the nontumorigenic A. vitis strain F2/5 (Li et al , 2005). The long‐chain AHL spot detected from F2/5 appears to be a mix of different AHLs encoded by the avsI locus that vary in oxo‐substitution on third carbon and in saturation of the acyl‐side chain (Li et al , 2005).…”

Section: Resultssupporting

confidence: 80%

“…Their growth on tartrate did not induce AHL production (data not shown). Biosensor strain NTL4 (pZLR4) detected a single AHL from vitopine‐type strain derived pTrS4 and pTrNW221 and octopine‐type‐strain derived pTrF2/5 (Li et al , 2005), which exhibited Rf values and detection characteristics identical to 3‐oxo‐C8‐HSL (Fig. 3b).…”

Section: Resultsmentioning

confidence: 99%

“…Agrobacterium vitis has more complex QS regulation systems. The nontumorigenic A. vitis strain F2/5 harbors both chromosomal‐ and plasmid‐encoded luxI/luxR type genes coding for at least six AHLs (Li et al , 2005), although little is known on the AHL production of various tumorigenic A. vitis isolates. To assess the QS systems in octopine‐, nopaline‐ and vitopine‐type A. vitis , we have studied a set of tumorigenic A. vitis strains.…”

Section: Discussionmentioning

confidence: 99%

“…In previous studies with A. vitis strains, two strains were negative and one produced a weakly detectable AHL signal (Cha et al , 1998). Tumorigenic A. vitis octopine‐type strains AB3 and Tm4 produced short‐ and long‐chain AHLs; in contrast, only long‐chain AHLs were found to be produced by the vitopine‐type strain S4 (Li et al , 2005). The nontumorigenic A. vitis strain F2/5 produced six AHLs encoded both by a chromosomal‐localized luxI ortholog, avsI , and by two putative luxI orthologs localized to the octopine‐, and tartrate‐catabolic plasmids (Li et al , 2005).…”

Section: Introductionmentioning

confidence: 96%

“…Tumorigenic A. vitis octopine‐type strains AB3 and Tm4 produced short‐ and long‐chain AHLs; in contrast, only long‐chain AHLs were found to be produced by the vitopine‐type strain S4 (Li et al , 2005). The nontumorigenic A. vitis strain F2/5 produced six AHLs encoded both by a chromosomal‐localized luxI ortholog, avsI , and by two putative luxI orthologs localized to the octopine‐, and tartrate‐catabolic plasmids (Li et al , 2005). Three QS systems of F2/5, namely aviR, avhR and avsI / avsR , regulate the induction of hypersensitive response on tobacco and tissue necrosis on grapevine (Hao et al , 2005; Hao & Burr, 2006).…”

Section: Introductionmentioning

confidence: 96%

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Quorum-sensing signal production byAgrobacterium vitisstrains and their tumor-inducing and tartrate-catabolic plasmids

Lowe

Gan

Chakravartty

et al. 2009

FEMS Microbiology Letters

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Agrobacterium vitis strains, their tumor-inducing (pTi) and tartrate utilization (pTr) plasmid transconjugants and grapevine tumors were analyzed for the presence of N-acyl-homoserine lactones (AHLs). All wild-type A. vitis strains produced long-chain signals. PCR analysis of the A. vitis long-chain AHL synthase gene, avsI, showed the predicted amplicon. Agrobacterium tumefaciens UBAPF2 harboring various A. vitis pTi plasmids produced N-(3-oxo-octanoyl)-l-homoserine lactone encoded also by pTis of A. tumefaciens. UBAPF2 transconjugants carrying pTrs except for pTrTm4 and pTrAB3, also produced an AHL. UBAPF2 transconjugants carrying pTrAT6, pTrAB4 and pTrRr4 or pTiNi1 produced two additional AHLs not observed in the corresponding wild-type strains. We also provide evidence for in situ production of AHLs in grapevine crown gall tumors of greenhouse and field origin.

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Section: Resultssupporting

confidence: 80%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 96%

See 3 more Smart Citations

Quorum-sensing signal production byAgrobacterium vitisstrains and their tumor-inducing and tartrate-catabolic plasmids

Lowe

Gan

Chakravartty

et al. 2009

FEMS Microbiology Letters

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Inhibition of quorum‐sensing signals by essential oils

Szabó

Varga

Hohmann

et al. 2009

Phytotherapy Research

125

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The role of quorum sensing (QS) is well known in microbial pathogenicity and antibiotic resistance. QS is responsible for motility, swarming, and biofilm production based on the signal molecules, e.g., acylated homoserine lactones (AHLs) produced by micro-organisms above certain population density. The inhibition of QS may reduce pathogenicity, antibiotic resistance and biofilm formation in systemic and local infections. The homoserine lactones and other transmitters contribute to antibiotic resistance and pathogenicity of several bacteria; consequently the inhibition of QS signals reduces the problem of resistance and virulence. Due to the increasing number of persistent non-treatable infections, there is an urgent need to develop new strategies to combat infections that destabilize bacterial communities in the host. The effect of essential oils on bacterial growth and QS were evaluated using the sensor strain Chromobacterium violaceum CV026 and N-acyl homoserine lactone (AHL) producing Escherichia coli ATTC 31298 and the grapevine colonizing Ezf 10-17 strains. Of the tested oils, rose, geranium, lavender and rosemary oils were the most potent QS inhibitors. Eucalyptus and citrus oils moderately reduced pigment production by CV026, whereas the chamomile, orange and juniper oils were ineffective.

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The Ecology of Agrobacterium vitis and Management of Crown Gall Disease in Vineyards

Kuzmanović

Puławska

Hao

et al. 2018

Current Topics in Microbiology and Immunology

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Agrobacterium vitis is the primary causal agent of grapevine crown gall worldwide. Symptoms of grapevine crown gall disease include tumor formation on the aerial plant parts, whereas both tumorigenic and nontumorigenic strains of A. vitis cause root necrosis. Genetic and genomic analyses indicated that A. vitis is distinguishable from the members of the Agrobacterium genus and its transfer to the genus Allorhizobium was suggested. A. vitis is genetically diverse, with respect to both chromosomal and plasmid DNA. Its pathogenicity is mainly determined by a large conjugal tumor-inducing (Ti) plasmid characterized by a mosaic structure with conserved and variable regions. Traditionally, A. vitis Ti plasmids and host strains were differentiated into octopine/cucumopine, nopaline, and vitopine groups, based on opine markers. However, tumorigenic and nontumorigenic strains of A. vitis may carry other ecologically important plasmids, such as tartrate- and opine-catabolic plasmids. A. vitis colonizes vines endophytically. It is also able to survive epiphytically on grapevine plants and is detected in soil exclusively in association with grapevine plants. Because A. vitis persists systemically in symptomless grapevine plants, it can be efficiently disseminated to distant geographical areas via international trade of propagation material. The use of healthy planting material in areas with no history of the crown gall represents the crucial measure of disease management. Moreover, biological control and production of resistant grape varieties are encouraging as future control measures.

show abstract

Chromosome and plasmid-encoded N-acyl homoserine lactones produced by Agrobacterium vitis wildtype and mutants that differ in their interactions with grape and tobacco

Cited by 12 publications

References 33 publications

Quorum-sensing signal production byAgrobacterium vitisstrains and their tumor-inducing and tartrate-catabolic plasmids

Quorum-sensing signal production byAgrobacterium vitisstrains and their tumor-inducing and tartrate-catabolic plasmids

Inhibition of quorum‐sensing signals by essential oils

The Ecology of Agrobacterium vitis and Management of Crown Gall Disease in Vineyards

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