Chromosomal location of DNA base sequences complementary to transfer RNA and to 5 s, 16 s and 23 s ribosomal RNA in Bacillus subtilis

Smith, Issar; Dubnau, David; Morrell, Peter L.; Marmur, Julius

doi:10.1016/0022-2836(68)90285-4

Cited by 146 publications

(58 citation statements)

References 31 publications

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“…Hybridization studies performed using density-transfer experiments for the localization of DNA regions first suggested that B. subtilis might have a type of tRNA gene organization different from that of E. coli in that the majority of tRNA genes of B. subtilis tended to be clustered in two regions of the genome, one near the origin of replication and one further away (1,2). Both regions also hybridized with the rRNAs for 5, 16, and 23S species, however, the majority of rRNA genes were clustered near the origin of replication at a position of about 3 to 12 (on a genetic map from 0-100).…”

Section: Introductionmentioning

confidence: 99%

“…Both regions also hybridized with the rRNAs for 5, 16, and 23S species, however, the majority of rRNA genes were clustered near the origin of replication at a position of about 3 to 12 (on a genetic map from 0-100). Early hybridization studies estimated the numbers of tRNA genes to be between 35 and 40 in B. subtilis (1,2). RNA-DNA hybridization studies by Jeng and Doi (3) suggest that there may be more tRNA genes than this.…”

Section: Introductionmentioning

confidence: 99%

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Sequence analysis of a cluster of twenty-one tRNA genes inBacillus subtilis

Green

Vold

1983

Nucl Acids Res

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Sequence analysis of a cluster of twenty-one tRNA genes inBacillus subtilis

Green

Vold

1983

Nucl Acids Res

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“…The minor clusters containing three or four of the rRNA cistrons are located in the late replicating portion of the Bacillus genome. Operons rrnB and rrnC have been mapped in the region between thr-5 and aroG (Bott et al, in press), At least one or two cistrons are believed to be located at the ilvBC-leu region (3,23; P. Gottlieb, G.…”

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confidence: 99%

Restriction Site Polymorphism of Ribosomal Ribonucleic Acid Gene Sets in Members of the Genus Bacillus

Gottlieb¹,

Rudner

1985

International Journal of Systematic Bacteriology

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Hybridization of cloned ribosomal sequences to EcoRI-restricted genomic deoxyribonucleic acids of eight species and strains of the genus Bacillus produced multiband patterns consistent with the presence of 9 to 11 operons per genome. The basic structure of the repeating ribosomal gene set is highly conserved with the exception of one internal EcoRI site located near the abutment region between the 16s and 23s ribosomal ribonucleic acid determinants. In each of the Bacillus species studied, there are two abutment regions that differ in size by 0.2 kilobase; the larger region contains genes for isoleucine and alanine transfer ribonucleic acids at one-third the proportion of the smaller region. The occurrence of the EcoRI site in strains of Bacillus subtilis and Bacillus lichenifurmis gave rise upon cleavage to 1.2-and 1.4-kilobase abutment families. The absence of the EcoRI site in Bacillus glubigii, Bacillus pumilus, and Bacillus amyloliquefaciens resulted in the emergence of 1.9-and 2.1-kilobase abutment families. Mixtures of the two types of families were not found in the Bacillus genomes studied.

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“…In Xenopus laevis, for example, the 5-S rRNA genes are found in a light satellite band in isopycnic CsCl gradients, whereas the genes coding for 28-S + 18-S rRNA are found as a heavy satellite [5]. A physical linkage between the genes for 5-S rRNA and those for high-molecularweight rRNA genes has been found only in bacteria [6,7] and in the lower eukaryote Sacchuromyces cerevisiae [B, 91. To advance our knowledge about the genetic information and the structural organization of the macronuclear DNA in Tetrahymena pyrijormis we have investigated the genes corresponding to tRNA and 5-S rRNA.…”

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confidence: 99%

Studies on the Amount and Location of the tRNA and 5-S rRNA Genes in Tetrahymena pyriformis GL

1976

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The amount and location of tRNA and 5-S rRNA genes in the macronucleus of Tetrahymena pyrformis GL was investigated by DNA-RNA hybridization. Hybridization of 32P-labelled tRNA in excess of unlabelled rRNA (25-S + 1 7 4 + 5-S) showed that at saturation 0.021 % of the macronuclear DNA was complementary to tRNA. Hybridization of 32P-labelled 5-S rRNA in excess of unlabelled 25-S + 17-S rRNA and tRNA showed a saturation value of 0.017%.In contrast to the 25-S + 17-S rRNA genes, which are found in DNA of high buoyant density, tRNA and 5-S rRNA genes were distributed evenly throughout the main peak observed when bulk macronuclear DNA was fractionated by density centrifugation in CsCl gradients. Sucrose gradient analyses of total macronuclear DNA showed that tRNA and 5-S rRNA genes were found in DNA of all size classes but a significant enrichment in the slowly sedimenting DNA fraction was observed.Saturation hybridization of 5-S rRNA to purified rDNA showed that rDNA did not contain any 5-S rRNA genes.In the past few years, our knowledge about the organizational and informational complexity of the eukaryotic genome has increased rapidly and for some specific genes, e.g. the genes coding for tRNA and 5-S rRNA, their location and organization within the eukaryotic chromosomal DNA has been rather well established recently.The tRNA genes are repetitive in most eukaryotes. Clarkson et al. [l] showed that each tRNA repeating unit in Xenopus laevis contains in addition to the transcribed DNA, approximately 90 % non-transcribed spacer DNA of unknown function. These repeating units are tandemly arranged in clusters and the genes for different isoaccepting tRNA molecules are arranged in separate clusters containing different spacer sequences [l].Brown et al. [2] showed that the repetitive 5-S rRNA genes of Xenopus laevis are highly clustered, and each repeating unit contains about 85% untranscribed spacer sequences. By hybridization in situ Pardue et al. [3] have shown that the 5-S rRNA genes are clustered in separate linkage groups at the telomers Although 5-S rRNA is a component of ribosomes, the genes for this RNA are normally unlinked to those for 2 5 3 + 17-S rRNA [4,5]. In Xenopus laevis, for example, the 5-S rRNA genes are found in a light satellite band in isopycnic CsCl gradients, whereas the genes coding for 28-S + 18-S rRNA are found as a heavy satellite [5]. A physical linkage between the genes for 5-S rRNA and those for high-molecularweight rRNA genes has been found only in bacteria [6,7] and in the lower eukaryote Sacchuromyces cerevisiae [B, 91. To advance our knowledge about the genetic information and the structural organization of the macronuclear DNA in Tetrahymena pyrijormis we have investigated the genes corresponding to tRNA and 5-S rRNA. The information obtained may hopefully contribute to a better understanding of the structure and location of specific genes on eukaryotic chromosomes.

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Chromosomal location of DNA base sequences complementary to transfer RNA and to 5 s, 16 s and 23 s ribosomal RNA in Bacillus subtilis

Cited by 146 publications

References 31 publications

Sequence analysis of a cluster of twenty-one tRNA genes inBacillus subtilis

Sequence analysis of a cluster of twenty-one tRNA genes inBacillus subtilis

Restriction Site Polymorphism of Ribosomal Ribonucleic Acid Gene Sets in Members of the Genus Bacillus

Studies on the Amount and Location of the tRNA and 5-S rRNA Genes in Tetrahymena pyriformis GL

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