Chromosomal localization of three humanras genes by in situ molecular hybridization

Popescu, Nicholas C.; Amsbaugh, Suzanne C.; DiPaolo, Joseph A.; Tronick, Steven R.; Aaronson, S A; Swan, David C.

doi:10.1007/bf01534703

Cited by 70 publications

(31 citation statements)

References 52 publications

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“…Theoretically, between 25 ± 50 genes could be present within such a stretch of DNA. However, SOX5, JAW1 and KRAS2 are the only genes which were so far mapped within this SROA (Popescu et al, 1985;Wunderle et al, 1996 and this manuscript). The human SOX5 gene was only very recently described.…”

Section: Discussionmentioning

confidence: 69%

Identification of the critical region of 12p over-representation in testicular germ cell tumors of adolescents and adults

et al. 1998

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Cytogenetically, testicular germ cell tumors of adolescents and adults (TGCTs) are characterized by gain of 12p-sequences, most often through isochromosome formation (i(12p)). Fluorescence in situ hybridization (FISH) has shown that i(12p))-negative TGCTs also cryptically contain extra 12p-sequences. The consistency of 12p-over-representation in all histological subtypes of TGCTs, including their preinvasive stage, suggests that gain of one or more genes on 12p is crucial in the development of this cancer. So far, studies aimed at the identi®cation of the relevant gene(s) were based on thè candidate-gene approach'. No convincing evidence in favor of or against a particular gene has been reported. We combined conventional karyotyping, comparative genomic hybridization, and FISH to identify TGCTs with ampli®cations of restricted regions of 12p. Out of 49 primary TGCTs (23 without i(12p), 13 with and 13 unknown), eight tumors (six without i(12p) and two unknown) showed ampli®cations corresponding to 12p11.1-p12.1. Using bicolour-FISH, physical mapping, and semi-quantitative polymerase chain reactions, the size of the shortest region of overlap of ampli®cation (SROA) was estimated to be between 1750 ± 3000 kb. In addition, we mapped a number of genes in and around this region. While fourteen known genes could be excluded as candidates based on their location outside this region, we demonstrate that KRAS2, JAW1 and SOX5 genes are localized within the SROA. While KRAS2 and JAW1 map to the proximal border of the SROA, SOX5 maps centrally in the SROA. KRAS2 and JAW1 are expressed in all TGCTs, whereas one 12p amplicon-positive TGCT lacks expression of SOX5. The critical region of 12p over-represented in TGCTs is less than 8% of the total length of the short arm of chromosome 12. It will be helpful in the identi®cation of the gene(s) involved in TGCT-development.

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Section: Discussionmentioning

confidence: 69%

Identification of the critical region of 12p over-representation in testicular germ cell tumors of adolescents and adults

et al. 1998

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“…The cellular and molecular mechanisms conferring oncogenic effects and activating KRAS mutations remain incompletely understood (Simi et al, 2008). Studies have showed that activation of the mitogen-activated protein kinase pathway was important for the development of colorectal cancer via BRAF or KRAS mutations (Popescu et al, 1985). Thus, in this study, we examined whether the BRAF and KRAS gene have the same effect in breast cancer.…”

Section: Introductionmentioning

confidence: 97%

Study of PIK3CA, BRAF, and KRAS mutations in breast carcinomas among Chinese women in Qinghai

Wang¹,

Dai²,

Li³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Phosphatidylinositol-3-OH kinase and RAS-activated signaling pathways play an important role in tumor formation. Abnormalities in relevant genes play essential roles in the occurrence and development of many human cancers. Studies of breast cancer have mainly focused on the women in western countries, but few studies have examined the frequency of mutations in PIK3CA, BRAF, and KRAS in Chinese breast cancer patients. In this study, we conducted sequence analysis of PIK3CA, BRAF, and KRAS and determined relationships with the occurrence of breast cancer in women from Qinghai. DNA was extracted from 25 cases of human breast cancer tissue samples. PIK3CA, BRAF, and KRAS mutation analysis was performed by polymerase chain reaction and DNA sequencing. No mutations were found in PIK3CA, BRAF, and KRAS of adjacent tissues. However, PIK3CA mutations were observed in 32% (8) of the 25 breast cancer tissues examined, in which exon 9 accounted for 4% (1), exon 20 accounted for 28% (7), and no mutations were found in exon 1 of PIK3CA. Sequencing of exon 2 of KRAS suggested that 20% (5) of the 25 samples harbored a mutation and 16% (4) 14840-14846 (2015) harbored a mutation. Any mutation in these 3 oncogenes may induce the occurrence and development of breast cancer.

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“…These less prominent fragments indicate the presence of additional JUN-like gene(s) on a different chromosome in the human genome. To confirm concordancy of human chromosome 1 and the major JUN 7.0-kbp fragment in the hybrid panel, filters were routinely stripped of probe and rehybridized to N-RAS and a-spectrin (SPTA) probes, which have been mapped to lp and lq, respectively (14,19 A summary of all somatic cell hybrid data is presented in Fig. 2.…”

Section: Resultsmentioning

confidence: 99%

Localization of the human JUN protooncogene to chromosome region 1p31-32.

Haluska

Huebner

Isobe

et al. 1988

Proc. Natl. Acad. Sci. U.S.A.

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The oncogene jun is the putative transforming gene of avian sarcoma virus 17; jun appears to be derived from a gene of the chicken genome and has homologues in several other vertebrate species. Recent genetic and immunological data indicate that jun codes for a protein that is closely related and probably identical to the transcription factor AP-1. We have isolated a genomic DNA clone encompassing the human cellular counterpart of the gene, JUN, and used this DNA to determine the chromosomal location of the gene. A panel of DNA preparations derived from rodent-human somatic cell hybrids with defined chromosome complements was first screened with the JUN probe. This Southern blot analysis indicated that JUN is situated on the short arm of chromosome 1. In situ hybridization then assigned JUN to chromosome region 1p31-32, a chromosomal region involved in both translocations and deletions of chromosomes seen in human malignancies.

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Chromosomal localization of three humanras genes by in situ molecular hybridization

Cited by 70 publications

References 52 publications

Identification of the critical region of 12p over-representation in testicular germ cell tumors of adolescents and adults

Identification of the critical region of 12p over-representation in testicular germ cell tumors of adolescents and adults

Study of PIK3CA, BRAF, and KRAS mutations in breast carcinomas among Chinese women in Qinghai

Localization of the human JUN protooncogene to chromosome region 1p31-32.

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