Chromatographic separation simulation of metal‐chelating peptides from surface plasmon resonance binding parameters

Abstract

Funding information LUE IMPACT BiomoleculesSome metal-chelating peptides have antioxidant properties, with potential nutrition, health, and cosmetics applications. This study aimed to simulate their separation on immobilized metal ion affinity chromatography from their affinity constant for immobilized metal ion determined in surface plasmon resonance, both technics are based on peptide-metal ion interactions. In our approach, first, the affinity constant of synthetic peptides was determined by surface plasmon resonance and used as input data to numerically simulate the chromatographic separation with a transport-dispersive model based on Langmuir adsorption isotherm. Then, chromatographic separation was applied on the same peptides to determine their retention time and compare this experimental t R with the simulated t R obtained from simulation from surface plasmon resonance data. For the investigated peptides, the relative values of t R were comparable. Hence, our study demonstrated the pertinence of such numerical simulation correlating immobilized metal ion affinity chromatography and surface plasmon resonance. K E Y W O R D Schelation, immobilized metal ion affinity chromatography, modeling, peptides, surface plasmon resonance

Screening, separation and identification of metal-chelating peptides for nutritional, cosmetics and pharmaceutical applications

“…Finally, the output data is the profile of peptide concentration as a function of time, from which the simulated retention time of a peptide is obtained, as reported in the proof of concept established by Muhr and colleagues. 210 On the other hand, experiments are done in IMAC, and experimental retention time is compared with the simulated retention time in order to validate the chromatography modelling. More recently, in our team, Irankunda et al (2024) deciphered the peptide-metal interaction involved in IMAC and SPR technology of 22 peptides.…”

“…209 The finding indicated that most of the studied peptides with a high retention time in IMAC also exhibited a high affinity in SPR, which agreed with Muhr and colleagues. 210 6. Chemical interaction between peptide and metal ions 6.1 Fourier-transform infrared spectroscopy Fourier-transform infrared spectroscopy (FTIR) gives information about the chemical composition and conformational structure of food components.…”

Review on preparation methods, mechanisms and applications for antioxidant peptides in oil

“…Catalytic metal can accelerate the decomposition of oil oxidation primary products to generate free radicals as shown in Scheme 4 (Muhr et al, 2020). The catalysis of catalytic metal depends on their ability to react with unoxidized oil to form hydroperoxides (Juita et al, 2011).…”

Electrically Switchable Nanolever Technology for the Screening of Metal-Chelating Peptides in Hydrolysates

“…In addition, surface plasmon resonance (SPR) was reported to compare the affinity constant of different tagged proteins bound to metal ions − or to study the affinity of His-tag peptides to Ni 2+ ions . Recently, our team developed a strategy to screen MCPs using affinity constants determined in SPR and to simulate their IMAC separation . Like SPR, the newly introduced switchSENSE technology allows to analyze biomolecular interactions in real time.…”

“…23 Recently, our team developed a strategy to screen MCPs using affinity constants determined in SPR 24 and to simulate their IMAC separation. 25 Like SPR, the newly introduced switchSENSE technology allows to analyze biomolecular interactions in real time. The originality of this latter technology is based on the use of electro-switchable fluorescent DNA nanolevers in which one DNA strand bears a fluorophore while its complementary strand is functionalized by a capture molecule (i.e., complexing agent), itself coupled to a ligand (e.g., metal ion).…”