Chromatographic resolution

Honda, Shiro; Hatäda, Koichi; Yuki, Heimei

doi:10.1016/s0021-9673(01)93512-5

Cited by 40 publications

(6 citation statements)

References 20 publications

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“…The CSP was prepared according to the procedure described in the literature. 11,12 CSP was packed into a 250 mm 3 4.6 mm (i.d.) stainless steel column.…”

Section: Hplc-dad Analysismentioning

confidence: 99%

Stereoselective degradation kinetics of tebuconazole in rabbits

et al. 2006

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Tebuconazole[(RS)-1-p-chlorophenyl-4,4-dimethyl-3-(1H-1,2,4-triazol-1-ylmethyl)pentan-3-ol] is a potent triazole fungicide and consists of a pair of enantiomers. The enantioselective degradation kinetics of tebuconazole was investigated in rabbits by intravenous (iv) injection. The concentrations of (-)-(R)-tebuconazole and (+)-(S)-tebuconazole in plasma and tissues were determined by HPLC with a cellulose tris(3,5-dimethylphenylcarbamate)-based chiral stationary phase. Enantioselective analysis methods for this fungicide in plasma and tissues were developed and validated. Good linearities were obtained over the concentration range of 0.25-25 mg/l for both enantiomers. The degradation followed pseudo-first-order kinetics and the degradation of the (+)-(S)-tebuconazole was much faster than that of the (-)-(R)-tebuconazole in plasma after administration of racemic tebuconazole. This study also indicated that environmental assessment of enantiomeric degradation may be needed to fully evaluate risks of tebuconazole use.

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“…The CSP was prepared according to the procedure described in the literature. 11,12 CSP was packed into a 250 mm 3 4.6 mm (i.d.) stainless steel column.…”

Section: Hplc-dad Analysismentioning

confidence: 99%

Stereoselective degradation kinetics of tebuconazole in rabbits

et al. 2006

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“…ATPC was synthesized according to the procedures described in the literature. 13,14 Dried amylopectin 1.0 g was dissolved in pyridine, stirred for 12 h at 1068C, 3.5 g of phenylisocyanate was added, and then the mixture was stirred for 48 h at 1068C. After cooling, 250 ml of methanol was added.…”

Section: Synthesis Of Atpc and Apsmentioning

confidence: 99%

The direct chiral separations of fungicide enantiomers on amylopectin based chiral stationary phase by HPLC

et al. 2006

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Amylopectin-tris(phenylcarbamate) was synthesized and coated to aminopropylsilica to prepare chiral stationary phase. The chiral separations of fungicide enantiomers were performed by the CSP using high-performance liquid chromatography. Mobile phase was n-hexane and isopropanol, and flow rate was 1.0 ml/min. Detection wavelength was 230 nm. The influence of the percentage of isopropanol in the mobile phase on the separations was studied. Twelve chiral fungicides were tested and seven of them were found to show stereoselectivity on the CSP. The enantiomers of metalaxyl and benalaxyl got near baseline separations and myclobutanil, hexconazole, tebuconazole, uniconazole, and paclobutrazol enantiomers were completely separated. The decreasing percentage of isopropanol in the mobile phase resulted in better separation and longer analysis time. The enantiomers were identified by a circular dichroism (CD) detector and the CD spectra of the individual enantiomers were also studied by online scanning.

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“…Polysaccharide derivatives-based chiral packing materials (CPMs), such as the phenylcarbamates, especially 3,5dimethylphenylcarbamates 1 and 2 in Figure 1, and benzoates of cellulose and amylose, exhibit high chiral recognition abilities toward a wide range of chiral compounds and have been recognized as the most powerful CPMs for direct separation of enantiomers by high-performance liquid chromatography (HPLC). [1][2][3][4][5][6] These polysaccharidebased CPMs have been traditionally prepared by coating the derivatives onto macroporous silica gel. Because of their coated nature, solvents such as tetrahydrofuran (THF) and chloroform, which can dissolve or swell the polysaccharide derivatives, cannot be used as eluents.…”

Section: Introductionmentioning

confidence: 99%

Immobilization and chiral recognition of 3,5‐dimethylphenylcarbamates of cellulose and amylose bearing 4‐(trimethoxysilyl)phenylcarbamate groups

2009

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A small amount of 4-(trimethoxysilyl)phenyl groups was randomly introduced onto the 3,5-dimethylphenylcarbamates of cellulose and amylose by a one-pot method. The obtained derivatives were then effectively immobilized onto silica gel as chiral packing materials (CPMs) for high-performance liquid chromatography through intermolecular polycondensation of the trimethoxysilyl groups. The effects of the amount of 4-(trimethoxysilyl)phenyl groups on immobilization and enantioseparation were investigated. Also, the solvent durability of the immobilized-type CPMs was examined with the eluents containing chloroform and tetrahydrofuran. When these eluents were used, the chiral recognition abilities of the CPMs for most of the tested racemates were improved to some extent depending on the compounds.

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Chromatographic resolution

Cited by 40 publications

References 20 publications

Stereoselective degradation kinetics of tebuconazole in rabbits

Stereoselective degradation kinetics of tebuconazole in rabbits

The direct chiral separations of fungicide enantiomers on amylopectin based chiral stationary phase by HPLC

Immobilization and chiral recognition of 3,5‐dimethylphenylcarbamates of cellulose and amylose bearing 4‐(trimethoxysilyl)phenylcarbamate groups

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