Chromatin-linked determinants of zygotic genome activation

Andersen, Ingrid S.; Collas, Philippe

doi:10.1007/s00018-012-1143-x

Cited by 28 publications

(22 citation statements)

References 98 publications

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“…The methylation status of H3K4 affects gene regulation in developing embryos (Gao et al, ; Ostrup et al, ). We observed more H3K4me and H3K4me2 in early‐ compared to late‐cleaving embryos during the EGA period, which implies that greater modification early in cleavage correlates with a higher probability of reaching the blastocyst stage.…”

Section: Discussionmentioning

confidence: 99%

“…A critical milestone of development is the maternal‐to‐zygote transition, the period when the embryo genome is activated (Wong et al, ; Lee et al, ); failure to undergo proper embryonic genome activation (EGA) leads to developmental arrest. Several epigenetic modifications and changes in chromatin structure occur during the EGA period (Ostrup et al, ; Lee et al, )—all of which are needed to regulate gene expression in developing embryos (Mason et al, ; Beaujean, , b; Cheedipudi et al, ). Removal of repressive marks and addition of activation marks, such as methylation of lysine 4 in histone 3 (H3K4me3), are among the epigenetic changes observed during EGA (Gao et al, ; Ostrup et al, ).…”

Section: Introductionmentioning

confidence: 99%

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Altered expression of BRG1 and histone demethylases, and aberrant H3K4 methylation in less developmentally competent embryos at the time of embryonic genome activation

Glanzner

Wachter

Coutinho

et al. 2016

Molecular Reproduction Devel

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Epigenetics is a fundamental regulator underlying many biological functions, such as development and cell differentiation. Epigenetic modifications affect key chromatin regulation, including transcription and DNA repair, which are critical for normal embryo development. In this study, we profiled the expression of epigenetic modifiers and patterns of epigenetic changes in porcine embryos around the period of embryonic genome activation (EGA). We observed that Brahma-related gene 1 (BRG1) and Lysine demethylase 1A (KDM1A), which can alter the methylation status of lysine 4 in histone 3 (H3K4), localize to the nucleus at Day 3-4 of development. We then compared the abundance of epigenetic modifiers between early- and late-cleaving embryos, which were classified based on the time to the first cell cleavage, to investigate if their nuclear localization contributes to developmental competence. The mRNA abundance of BRG1, KDM1A, as well as other lysine demethylases (KDM1B, KDM5A, KDM5B, and KDM5C), were significantly higher in late- compared to early-cleaving embryos near the EGA period, although these difference disappeared at the blastocyst stage. The abundance of H3K4 mono- (H3K4me) and di-methylation (H3K4me2) during the EGA period was reduced in late-cleaving and less developmentally competent embryos. By contrast, BRG1, KDM1A, and H3K4me2 abundance was greater in embryos with more than eight cells at Day 3-4 of development compared to those with fewer than four cells. These findings suggest that altered epigenetic modifications of H3K4 around the EGA period may affect the developmental capacity of porcine embryos to reach the blastocyst stage. Mol. Reprod. Dev. 84: 19-29, 2017. © 2016 Wiley Periodicals, Inc.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Altered expression of BRG1 and histone demethylases, and aberrant H3K4 methylation in less developmentally competent embryos at the time of embryonic genome activation

Glanzner

Wachter

Coutinho

et al. 2016

Molecular Reproduction Devel

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“…The nature of the relationship between zygotic transcription and chromatin regulation has been uncertain. Chromatin regulation could precede and initiate zygotic transcription (Meier et al, 2018;Østrup et al, 2013). Conversely, zygotic transcription may help shape chromatin structure, a model that has been tested by several studies: A study that analyzed the spatial genome organization in fly embryos revealed that early expressed genes correlate with the boundaries of Topologically Associating Domains (TADs).…”

Section: Zygotic Transcription and Chromatin Organizationmentioning

confidence: 99%

MET-2, a SETDB1 family methyltransferase, coordinates embryo events through distinct histone H3 methylation states

Mutlu

Chen

Hall³

2018

Preprint

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STATEMENTDuring early embryogenesis, heterochromatin formation and loss of developmental plasticity are coordinately regulated by distinct Histone H3 Lysine 9 (H3K9) methylation states, by the methyltransferase MET-2. ABSTRACTDuring the first hours of embryogenesis, formation of higher-order heterochromatin coincides with the loss of developmental potential. Here we examine the relationship between these two processes, and we probe the determinants that contribute to their onset. Mutations that disrupt histone H3 lysine 9 (H3K9) methyltransferases reveal that the methyltransferase MET-2 helps terminate developmental plasticity, likely through mono-and di-methylation of H3K9 (me1/me2), and promotes heterochromatin formation, likely through H3K9me3. We examine how MET-2 is regulated and find that methylated H3K9 appears gradually and depends on the accumulated time of embryogenesis.H3K9me is independent of zygotic genome activation or cell counting. These data reveal how central events are synchronized during embryogenesis and distinguish distinct roles for different H3K9 methylation states.

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“…During the first cell cycles of the zebrafish embryos, some histone marks such as H3K4 (H3: H3 family of histones; K: standard abbreviation for lysine; 4: position of amino acid residue counting from the N-terminus), H3K9 or H3K27 were acquired for epigenetic reprogramming [203,231]. Cayuso et al reviewed the roles of chromatin modifications in zebrafish development and regeneration [232].…”

Section: Epigenetic Effects Of Ionizing Radiation In Zebrafishmentioning

confidence: 99%

Zebrafish as an In Vivo Model to Assess Epigenetic Effects of Ionizing Radiation

Kong

Cheng

2016

IJMS

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Exposure to ionizing radiations (IRs) is ubiquitous in our environment and can be categorized into “targeted” effects and “non-targeted” effects. In addition to inducing deoxyribonucleic acid (DNA) damage, IR exposure leads to epigenetic alterations that do not alter DNA sequence. Using an appropriate model to study the biological effects of radiation is crucial to better understand IR responses as well as to develop new strategies to alleviate exposure to IR. Zebrafish, Danio rerio, is a scientific model organism that has yielded scientific advances in several fields and recent studies show the usefulness of this vertebrate model in radiation biology. This review briefly describes both “targeted” and “non-targeted” effects, describes the findings in radiation biology using zebrafish as a model and highlights the potential of zebrafish to assess the epigenetic effects of IR, including DNA methylation, histone modifications and miRNA expression. Other in vivo models are included to compare observations made with zebrafish, or to illustrate the feasibility of in vivo models when the use of zebrafish was unavailable. Finally, tools to study epigenetic modifications in zebrafish, including changes in genome-wide DNA methylation, histone modifications and miRNA expression, are also described in this review.

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Chromatin-linked determinants of zygotic genome activation

Cited by 28 publications

References 98 publications

Altered expression of BRG1 and histone demethylases, and aberrant H3K4 methylation in less developmentally competent embryos at the time of embryonic genome activation

Altered expression of BRG1 and histone demethylases, and aberrant H3K4 methylation in less developmentally competent embryos at the time of embryonic genome activation

MET-2, a SETDB1 family methyltransferase, coordinates embryo events through distinct histone H3 methylation states

Zebrafish as an In Vivo Model to Assess Epigenetic Effects of Ionizing Radiation

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