Chromate reductase activity in Streptomyces sp. MC1

Polti, Marta Alejandra; Amoroso, Maria Julia del R.; Abate, Carlos Mauricio

doi:10.2323/jgam.56.11

Cited by 55 publications

(34 citation statements)

References 41 publications

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“…In turn, biological transformation of Cr(VI) to Cr (III) by enzymatic reduction has been recognized as a means of chromium decontamination from effluents (Laxman and More 2002;Polti et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Intracellular chromium accumulation by Streptomyces sp. MC1

Polti

Amoroso

Abate

2010

Water Air Soil Pollut

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Streptomyces sp. MC1, previously isolated from sugar cane, has shown ability to reduce Cr(VI) in liquid minimal medium and soil samples. The objective of this work was to demonstrate the intracellular chromium accumulation by Streptomyces sp. MC1 under different culture conditions. This strain was able to accumulate up to 3.54 mg of Cr (III) per gram of wet biomass, reducing the 98% of Cr (VI) and removing 13.9% of chromium from the culture medium supernatants. Streptomyces sp. MC1 chromium bioaccumulation ability was corroborated by using Timm's reagent technique, a low-cost method, which has been used by first time to detect chromium deposits in bacteria. The results of atomic absorption spectrometry, scanning electron microscopy, and energy dispersive X-ray analysis suggest that the mechanism of Cr(VI) resistance observed in Streptomyces sp. MC1 includes adsorption coupled with reduction to Cr(III), and finally, Cr(III) bioaccumulation. This mechanism have special relevance to remediation of Cr(VI) contaminated environments by Streptomyces sp. MC1.

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Section: Introductionmentioning

confidence: 99%

Intracellular chromium accumulation by Streptomyces sp. MC1

Polti

Amoroso

Abate

2010

Water Air Soil Pollut

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“…Several studies have indicated a correlation between multiple antibiotic Science Publications AJBB resistance and heavy metal resistance (Bahig et al, 2008;Spain, 2003). Heavy metal resistance together with metabolic diversity and specific growth characteristics of actinomycetes, such as, mycelium formation and relatively rapid colonization of selective substrates indicate them as suitable agents for bioremediation (Polti et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

“…Cr(VI) is highly water soluble and more mobile, thus spreads easily beyond the site of initial contamination, while Cr(III) is relatively inert, less mobile, less bioavailable and easily adsorbed on mineral surfaces. Besides, hexavalent chromium compounds are approximately 100 fold more toxic and 1000 fold more mutagenic than trivalent chromium (Polti et al, 2010;2007). Chromium containing effluents from industries like leather tanning, chrome-plating, wood preservation, dye industry are released directly or indirectly into natural water resources, mostly without proper treatment which renders chromium associated environmental pollution, a major concern.…”

Section: Introductionmentioning

confidence: 99%

“…(Asatiani et al, 2004). However, there are only a few studies on Cr(VI) resistance and bioreduction by actinomycetes (Polti et al, 2010;2007). The first report on Cr(VI) reduction by Streptomyces was documented by Das and Chandra (1990).…”

Section: Introductionmentioning

confidence: 99%

“…Polti et al (2009), determined Cr(VI) reduction by Streptomyces sp. MC1 and later on characterize its chromate reductase activity (Polti et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

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Antibiotic Resistance and Chromium Reduction Pattern Among Actinomycetes

Ali¹

2012

American Journal of Biochemistry and Biotechnology

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Actinomycetes, one of the most important groups of microbes, exhibit many interesting activities such as degradation and transformation of organic and metal substrates together with production of antibiotics. With these bioactivities, actinomycetes would play an important role in the webs of the marine environment. The present study was designed to evaluate the antibiotic resistance pattern, antibiotic producing potential and chromium resistance as well as chromium reduction potential of a range of actinomycetes isolated from marine environments. Actinomycetes were isolated from marine sediment samples obtained from St. Martin's Island in Bangladesh. Antibiotic resistance among the selected isolates was studied against 10 different antibiotics by disc diffusion method and antibiotic producing potential was assessed by the perpendicular streak method. The isolates were screened for resistance towards heavy metal Cr(VI) on culture plates supplemented with Cr(VI) at concentrations ranging from 1-5 mM of Cr(VI). Highly resistant isolates were subjected to screening for Cr(VI) reduction activity, which was estimated using the Cr(VI) specific colorimetric reagent 1, 5-diphenylcarbazide. Out of the total 30 different selected isolates, 25 (83.33%) showed resistance against more than three antibiotics and 6 (20%) showed resistance to more than six antibiotics. Ninety three percent of the isolates showed MAR index greater than 0.2 and tolerance to Cr(VI) at 1mM of initial Cr(VI). None of the isolates displayed antimicrobial activity against the organisms tested. Among the isolates tested for chromate reduction, two were most efficient showing complete reduction of 1mM Cr(VI) within 24 h. These two isolates were capable of reducing chromate even at high initial Cr(VI) concentrations. Remarkably, the isolate SM-11 was found to reduce 82.67%, 44.34% of Cr(VI) at 2.5mM, 5mM of initial Cr(VI) concentrations respectively, within 72h of incubation. The majority of the actinomycetes isolates displayed resistance to both antibiotics and heavy metal chromium which indicates the possible acquisition of resistance factors due to environmental or human activities. The study also demonstrates possible correlation between antibiotic resistance and metal tolerance. Two of the isolates which showed considerable chromium reduction activity even at high chromium concentrations, may find potential application in bioremediation approaches.

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Isolation and process parameter optimization of Brevibacterium casei for simultaneous bioremediation of hexavalent chromium and pentachlorophenol

Verma

Singh

2012

J. Basic Microbiol

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Chromate and pentachlorophenol are major pollutants discharged through tanneries. Three bacteria resistant to high Cr6+ and PCP concentrations simultaneously were isolated. The TVS‐3 strain was tolerant to highest 850 mg l−1 Cr6+ and 1000 mg l−1 PCP concentration and concomitantly reduced 69% Cr6+ and degraded 72% PCP within 168 h at pH 7.5, 35 ± 1°C temperature, was selected and identified as Brevibacterium casei. At 168 h of growth, bacterium showed maximum PCP utilization of 720 mg l−1 and released 900 mg l−1 chloride ion. The bacterium exhibited remarkable ability to significantly reduce Cr6+ and degrade PCP in presence of other metals, between 100–120 rpm aeration and over broad pH (6.5–10.0) and temperature (30–40°C) range. Maximum 78% Cr6+ reduction and 82% PCP degradation was observed at pH 8.0, 35 ± 1°C within 168 h of incubation, 120 rpm and initial concentration of 850 mg l−1 Cr6+ and 1000 mg l−1 PCP. This is the first study reporting 78% Cr6+ reduction and 82% PCP degradation simultaneously by single native bacteria under wide growth conditions utilizing PCP as sole carbon source. This bacterium may potentially be useful for simultaneous bioremediation of Cr6+ and PCP containing wastes in the environment.

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Chromate reductase activity in Streptomyces sp. MC1

Cited by 55 publications

References 41 publications

Intracellular chromium accumulation by Streptomyces sp. MC1

Intracellular chromium accumulation by Streptomyces sp. MC1

Antibiotic Resistance and Chromium Reduction Pattern Among Actinomycetes

Isolation and process parameter optimization of Brevibacterium casei for simultaneous bioremediation of hexavalent chromium and pentachlorophenol

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