Choline Chloride Based Natural Deep Eutectic Solvents as Extraction Media for Extracting Phenolic Compounds from Chokeberry (Aronia melanocarpa)

Razboršek, Maša Islamčević; Ivanović, Milena; Krajnc, Peter; Kolar, Mitja

doi:10.3390/molecules25071619

Cited by 49 publications

(17 citation statements)

References 54 publications

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“…The most intriguing result in our study came from the extraction with a mixture of choline chloride and glycerol (Ch1G2), which extracted stilbenoids with a yield comparable to that of methanol extraction and acceptable stilbenoid stability. Similarly, in earlier reports choline chloride-based NADES exhibited efficiency in extracting phenolic compounds [18,19]. According to the results of a skin irritation test, Ch1G2 is safe for leave-on cosmetics.…”

Section: Capacity Of Nades For M Alba Callus Extractionsupporting

confidence: 60%

Preparation of Readily-to-Use Stilbenoids Extract from Morus alba Callus Using a Natural Deep Eutectic Solvent

et al. 2021

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The consumer and cosmetic industries have recently placed a greater emphasis on ecofriendly solvents for botanical extraction, including natural deep eutectic solvents (NADES). In this study, NADES were prepared for Morus alba callus extraction. The efficiency of extraction from the NADES and methanol was investigated by comparison of the stilbenoids yield and anti-melanogenesis activity. Prior to testing the irritability of a suitable NADES on the reconstructed human epidermis (RhE), the effect of the selected NADES on stilbenoids stability was determined. The results showed that the highest yields of stilbenoids were obtained from choline chloride-glycerol mixtures (Ch1G2) and methanol extracts, with no significant difference in yields (5.06 ± 0.05 and 6.32 ± 0.40 mg/g callus dry weight, respectively). The NADES extracts of M. alba callus showed comparable anti-melanogenesis activity compared to methanol. In term of stability, stilbenoids in Ch1G2 remained stable after six months of storage at 4 °C except resveratrol. Furthermore, Ch1G2 had no irritation effect on RhE. Thus, based on the findings of this study, Ch1G2 is an intriguing green solvent alternative for the extraction of M. alba callus and may be advantageous for the preparation of skin-lightening cosmetics.

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Section: Capacity Of Nades For M Alba Callus Extractionsupporting

confidence: 60%

Preparation of Readily-to-Use Stilbenoids Extract from Morus alba Callus Using a Natural Deep Eutectic Solvent

et al. 2021

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“…The availability, low cost, biodegradability, and environmental friendliness of the components makes the NADESs versatile alternatives to conventional organic solvents [18]. Recently, several studied have used NADESs for extraction and separation of different types of bioactive compounds, such as phenolic acids, flavonoids, and alkaloids from various plant materials [19][20][21]. Nonetheless, the number of reports on the application of NADESs for the extraction of bioactive compounds is still limited, and the efficiency of NADESs on extraction of bioactive steroidal saponins from DNR still remains unknown.…”

Section: Introductionmentioning

confidence: 99%

Natural Deep Eutectic Solvents for the Extraction of Bioactive Steroidal Saponins from Dioscoreae Nipponicae Rhizoma

et al. 2021

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In the present study, a simple and environmentally friendly extraction method based on natural deep eutectic solvents (NADESs) was established to extract four bioactive steroidal saponins from Dioscoreae Nipponicae Rhizoma (DNR). A total of twenty-one types of choline chloride, betaine, and L-proline based NADESs were tailored, and the NADES composed of 1:1 molar ratio of choline chloride and malonic acid showed the best extraction efficiency for the four steroidal saponins compared with other NADESs. Then, the extraction parameters for extraction of steroidal saponins by selected tailor-made NADES were optimized using response surface methodology and the optimal extraction conditions are extraction time, 23.5 min; liquid–solid ratio, 57.5 mL/g; and water content, 54%. The microstructure of the DNR powder before and after ultrasonic extraction by conventional solvents (water and methanol) and the selected NADES were observed using field emission scanning electron microscope. In addition, the four steroidal saponins were recovered from NADESs by D101 macroporous resin with a satisfactory recovery yield between 67.27% and 79.90%. The present research demonstrates that NADESs are a suitable green media for the extraction of the bioactive steroidal saponins from DNR, and have a great potential as possible alternatives to organic solvents for efficiently extracting bioactive compounds from natural products.

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“…Many publications have investigated phenolic compounds—constituents of various plants—in terms of their role as natural antioxidants. It is well known that phenolic compounds have many more bioactive applications, such as anti-inflammatory, anti-tumor, antimicrobial and obesity-inhibiting functions, and have therefore, received a great deal of attention from scientists in various research areas over the past decades [ 1 ]. Consequently, natural plant extracts enriched with different classes of phenolic compounds (flavonoids, anthocyanins, phenolic acids, etc.)…”

Section: Introductionmentioning

confidence: 99%

Validated Stability-Indicating GC-MS Method for Characterization of Forced Degradation Products of Trans-Caffeic Acid and Trans-Ferulic Acid

2021

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When dealing with simple phenols such as caffeic acid (CA) and ferulic acid (FA), found in a variety of plants, it is very important to have control over the most important factors that accelerate their degradation reactions. This is the first report in which the stabilities of these two compounds have been systematically tested by exposure to various different factors. Forced degradation studies were performed on pure standards (trans-CA and trans-FA), dissolved in different solvents and exposed to different oxidative, photolytic and thermal stress conditions. Additionally, a rapid, sensitive, and selective stability-indicating gas chromatographic-mass spectrometric method was developed and validated for determination of trans-CA and trans-FA in the presence of their degradation products. Cis-CA and cis-FA were confirmed as the only degradation products in all the experiments performed. All the compounds were perfectly separated by gas chromatography (GC) and identified using mass spectrometry (MS), a method that additionally elucidated their structures. In general, more protic solvents, higher temperatures, UV radiation and longer storage times led to more significant degradation (isomerization) of both trans-isomers. The most progressive isomerization of both compounds (up to 43%) was observed when the polar solutions were exposed to daylight at room temperature for 1 month. The method was validated for linearity, precision as repeatability, limit of detection (LOD) and limit of quantitation (LOQ). The method was confirmed as linear over tested concentration ranges from 1−100 mg L−1 (r2s were above 0.999). The LOD and LOQ for trans-FA were 0.15 mg L−1 and 0.50 mg L−1, respectively. The LOD and LOQ for trans-CA were 0.23 mg L−1 and 0.77 mg L−1, respectively.

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Choline Chloride Based Natural Deep Eutectic Solvents as Extraction Media for Extracting Phenolic Compounds from Chokeberry (Aronia melanocarpa)

Cited by 49 publications

References 54 publications

Preparation of Readily-to-Use Stilbenoids Extract from Morus alba Callus Using a Natural Deep Eutectic Solvent

Preparation of Readily-to-Use Stilbenoids Extract from Morus alba Callus Using a Natural Deep Eutectic Solvent

Natural Deep Eutectic Solvents for the Extraction of Bioactive Steroidal Saponins from Dioscoreae Nipponicae Rhizoma

Validated Stability-Indicating GC-MS Method for Characterization of Forced Degradation Products of Trans-Caffeic Acid and Trans-Ferulic Acid

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