2004
DOI: 10.1016/j.bbamem.2004.03.010
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Abstract: The serotonin(1A) (5-HT(1A)) receptor is an important member of the superfamily of seven-transmembrane domain G-protein-coupled receptors. We have examined the modulatory role of cholesterol on the ligand binding activity and G-protein coupling of the bovine hippocampal 5-HT(1A) receptor by depleting cholesterol from native membranes using methyl-beta-cyclodextrin (MbetaCD). Removal of cholesterol from bovine hippocampal membranes using varying concentrations of MbetaCD results in a concentration-dependent red… Show more

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Cited by 228 publications
(202 citation statements)
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“…Knowledge of dynamics would help in the analysis of functional data generated by modulation of the membrane lipid composition. 10 Our results show that the polarity of the hippocampal membrane is increased upon cholesterol depletion, as monitored by the change in the ratio of the pyrene vibronic band intensities. This is accompanied by an increase in lateral diffusion, measured as an increase in the pyrene excimer/monomer ratio.…”
Section: Resultsmentioning
confidence: 57%
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“…Knowledge of dynamics would help in the analysis of functional data generated by modulation of the membrane lipid composition. 10 Our results show that the polarity of the hippocampal membrane is increased upon cholesterol depletion, as monitored by the change in the ratio of the pyrene vibronic band intensities. This is accompanied by an increase in lateral diffusion, measured as an increase in the pyrene excimer/monomer ratio.…”
Section: Resultsmentioning
confidence: 57%
“…10 Figure 2 shows that, upon treatment with increasing concentrations of M CD, the cholesterol content of hippocampal membranes shows progressive reduction. Thus, when native membranes are treated with 10 mM M CD, the cholesterol content is reduced to ∼87% of the initial value.…”
Section: Resultsmentioning
confidence: 98%
“…1b). These results suggest that the BODIPY moiety of the probe is localized at the interfacial region of the membrane, a region characterized by unique dynamics and dielectric properties (Haldar et al, 2011).We have previously shown that cholesterol content in hippocampal membranes can be efficiently modulated using MβCD as it forms a selective inclusion complex with cholesterol by including it in the central nonpolar cavity (Pucadyil and Chattopadhyay, 2004). Fig.…”
mentioning
confidence: 92%
“…More importantly, the function of neuronal receptors depends on cholesterol (Pucadyil and Chattopadhyay, 2006;Allen et al, 2007;Paila and Chattopadhyay, 2010;Jafurulla and Chattopadhyay, 2013), which affects neurotransmission, resulting in mood and anxiety disorders (Papakostas et al, 2004). In spite of these important functional correlates, the organization and dynamics of neuronal membranes as a consequence of alterations in membrane cholesterol is only beginning to be understood.We have earlier established native hippocampal membranes as a convenient natural source for exploring the interaction of neuronal receptors such as the serotonin1A receptor, with membrane lipids of neuronal origin (Pucadyil and Chattopadhyay, 2004). In this overall context, we have demonstrated the requirement of membrane cholesterol in modulating the function of the serotonin1A receptor Chattopadhyay, 2004, 2006;Paila et al, 2008;Paila and Chattopadhyay, 2010;Shrivastava et al, 2010;Jafurulla and Chattopadhyay, 2013).…”
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confidence: 97%
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