Cholesterol alters the inhibitory efficiency of peptide-based membrane fusion inhibitor

Pattnaik, Gourab Prasad; Chakraborty, Hirak

doi:10.1016/j.bbamem.2019.183056

Cited by 24 publications

(64 citation statements)

References 69 publications

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“…It may be noted that a WD-containing 23 amino acid peptide (TG-23) exhibited significantly less inhibition of fusion when 20 mol % cholesterol was incorporated in the membrane. [39] We conclude that the hydrophobic anchor is important, and myristoyl chain is the smallest anchor to exert reasonable inhibition of fusion. Therefore, further experiments were performed with myristoyl chains.…”

Section: Resultsmentioning

confidence: 75%

Translation of Mycobacterium Survival Strategy to Develop a Lipo‐peptide based Fusion Inhibitor**

et al. 2021

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The entry of enveloped viruses requires the fusion of viral and host cell membranes. An effective fusion inhibitor aiming at impeding such membrane fusion may emerge as a broad‐spectrum antiviral agent against a wide range of viral infections. Mycobacterium survives inside the phagosome by inhibiting phagosome‐lysosome fusion with the help of a coat protein coronin 1. Structural analysis of coronin 1 and other WD40‐repeat protein suggest that the trp‐asp (WD) sequence is placed at distorted β‐meander motif (more exposed) in coronin 1. The unique structural feature of coronin 1 was explored to identify a simple lipo‐peptide sequence (myr‐WD), which effectively inhibits membrane fusion by modulating the interfacial order, water penetration, and surface potential. The mycobacterium inspired lipo‐dipeptide was successfully tested to combat type 1 influenza virus (H1N1) and murine coronavirus infections as a ‘potential broad‐spectrum’ antiviral agent.

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Section: Resultsmentioning

confidence: 75%

Translation of Mycobacterium Survival Strategy to Develop a Lipo‐peptide based Fusion Inhibitor**

et al. 2021

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“…At aChol-WD to lipid ratio of 1:100, the addition of peptide will increase the cholesterol content by anegligible amount of about 1mol %inthe membrane.It may be noted that aW D-containing 23 amino acid peptide (TG-23) exhibited significantly less inhibition of fusion when 20 mol %c holesterol was incorporated in the membrane. [39] We conclude that the hydrophobic anchor is important, and myristoyl chain is the smallest anchor to exert reasonable inhibition of fusion. Therefore,f urther experiments were performed with myristoyl chains.N ext, the roles of tryptophan and aspartic acid were investigated.…”

Section: Angewandte Chemiementioning

confidence: 75%

Translation of Mycobacterium Survival Strategy to Develop a Lipo‐peptide based Fusion Inhibitor**

et al. 2021

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The entry of enveloped virus requires the fusion of viral and host cell membranes. An effective fusion inhibitor aiming at impeding such membrane fusion may emerge as a broad‐spectrum antiviral agent against a wide range of viral infections. Mycobacterium survives inside the phagosome by inhibiting phagosome–lysosome fusion with the help of a coat protein coronin 1. Structural analysis of coronin 1 and other WD40‐repeat protein suggest that the trp‐asp (WD) sequence is placed at distorted β‐meander motif (more exposed) in coronin 1. The unique structural feature of coronin 1 was explored to identify a simple lipo‐peptide sequence (myr‐WD), which effectively inhibits membrane fusion by modulating the interfacial order, water penetration, and surface potential. The mycobacterium inspired lipo‐dipeptide was successfully tested to combat type 1 influenza virus (H1N1) and murine coronavirus infections as a potential broad‐spectrum antiviral agent.

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“…This prompted us to develop certain class of peptides or small molecules that inhibit the structural changes in the membrane that is essential for fusion process. In search of peptide-based broad-spectrum antiviral agent by targeting the membrane, we recently developed coronin 1-derived tryptophan–aspartic acid (WD)-containing peptides, which show significant inhibition of polyethylene glycol (PEG)-mediated fusion of model membranes (Pattnaik and Chakraborty 2018 , 2019 ).…”

Section: Broad-spectrum Antiviral Agents: Need Of the Hourmentioning

confidence: 99%

Entry Inhibitors: Efficient Means to Block Viral Infection

Pattnaik

Chakraborty

2020

J Membrane Biol

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Cholesterol alters the inhibitory efficiency of peptide-based membrane fusion inhibitor

Cited by 24 publications

References 69 publications

Translation of Mycobacterium Survival Strategy to Develop a Lipo‐peptide based Fusion Inhibitor**

Translation of Mycobacterium Survival Strategy to Develop a Lipo‐peptide based Fusion Inhibitor**

Translation of Mycobacterium Survival Strategy to Develop a Lipo‐peptide based Fusion Inhibitor**

Entry Inhibitors: Efficient Means to Block Viral Infection

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