Chlorpyrifos degradation by the cyanobacterium Synechocystis sp. strain PUPCCC 64

Singh, Devinder; Khattar, J. I. S.; Nadda, J.; Singh, Yadvinder; Garg, Anita; Kaur, Nirmaljit; Gulati, Arvind

doi:10.1007/s11356-011-0472-x

Cited by 104 publications

(42 citation statements)

References 43 publications

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“…KK26 was isolated from a paddy field where the pre-emergence herbicide pretilachlor is used on a large scale. ecosystem, it is necessary to examine the effect of agricultural pollutants such as pesticides on the growth, development and nitrogen assimilation of cyanobacteria (Singh et al 2011). Desmonostoc muscorum PUPCCC 405.10 tolerated herbicide up to 10 ppm which is 13.3 times the recommended field application dose (Fig.…”

Section: Discussionmentioning

confidence: 99%

Effect of pretilachlor on nitrogen uptake and assimilation by the cyanobacterium Desmonostoc muscorum PUPCCC 405.10

et al. 2015

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Tolerance limit, kinetics of nitrogen uptake and activity of nitrate reductase (NR), nitrite reductase (NiR) and glutamine synthetase (GS) enzymes of cyanobacterium Desmonostoc muscorum PUPCCC 405.10 were studied under the regime of chloroacetanilide herbicide pretilachlor. The organism was isolated from the paddy field under application of the herbicide and tolerated pretilachlor up to 10 ppm under laboratory conditions. The incubation of the cyanobacterium in 2.5 ppm pretilachlor did not cause significant effect on N uptake while supplementation of 5-10 ppm pretilachlor in medium reduced the rate of nitrate and nitrite uptake by 35-73 % with 50 % decrease in V max and no change in K m . These results indicated that herbicide did not affect the affinity of uptake system to nitrate and nitrite. In presence of herbicide, the activity of nitrogen assimilation enzymes was inhibited by 16 % for NR and 18 % for NiR. Unchanged K m and decreased V max (50-60 %) in presence of herbicide indicated non-competitive-type inhibition of the enzymes. A 50 % decrease in V max and K m of ammonium uptake indicated un-competitive type inhibition of the ammonium transport system by the herbicide. GS activity also exhibited non-competitive inhibition in presence of pretilachlor with 21 % decrease in activity with unchanged K m and 40 % decrease in V max . Likewise, decreases in nitrogenase activity by 43 % and heterocyst formation by 40 % were recorded in presence of herbicide. Results indicated that pretilachlor affected nitrogen assimilation at uptake level and interacted with nitrogen-assimilating enzymes in a non-competitive manner.

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Section: Discussionmentioning

confidence: 99%

Effect of pretilachlor on nitrogen uptake and assimilation by the cyanobacterium Desmonostoc muscorum PUPCCC 405.10

et al. 2015

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“…A major chloropyrifos metabolite, 3,5,6-trichloro-2-pyridinol (TCP), has been identified in the degradation process by cyanobacterium Synechocystis sp. strain PUPCCC 64 [2]. Another strain of Alcaligenes sp., capable of degrading chlorpyrifos and its major metabolite TCP, has been isolated from paddy field soil [45].…”

Section: Substrate Enzyme Activitiesmentioning

confidence: 99%

“…Chlorpyrifos, a broad spectrum organophosphate insecticide, is applied on a large scale in croplands for the control of foliar insects [1][2][3]. Approximately 140,000 t of chlorpyrifos are applied annually on a global scale, accounting for 1/3 of total organophosphorus pesticide use.…”

Section: Introductionmentioning

confidence: 99%

Mitigation of Wastewater-Borne Chorpyrifos in Constructed Wetlands: an Ecological Suitability Assessment by Macrophyte and Microbial Responses

Wang

Liu

et al. 2017

Pol. J. Environ. Stud.

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Plants and microorganisms are the main biotic compartments for phytoaccumulation and metabolic transformation of organic contaminants in constructed wetlands (CWs). However, how they cope with special pollutants during the treatment process has not been well characterized. In this study, responses of Iris pseudoacorus (I. pseudoacorus) and microbial communities were intensively investigated in pilot-scale CWs treating wastewater-borne chlorpyrifos, an organic phosphorus pesticide. Chlorpyrifos was associated with inhibited plant growth, decreased photosynthetic activity, and a significant increase in oxidative products. Superoxide dismutase (SOD) and ascorbate peroxidase (APX) activities were affected by chlorpyrifos, whereas catalase (CAT) activity was almost unaffected. Although chlorpyrifos stimulated the antioxidant system, there was little indication of oxidative damage in I. pseudoacorus. Urease, β-glucosidase, and phosphatase activities in substrate were elevated by 73.73%, 17.20%, and 16.23%, respectively, which may indicate enhancement of nitrogen, carbon, and phosphorus cycling. Fatty acid methyl ester (FAME) profiles showed that aerobic prokaryotes, which are likely the functional group responsible for chlorpyrifos degradation, increased from 16.37% to 31.32% after chlorpyrifos addition. Biomarkers for fungal/bact and trans/cis remained unchanged after the chlorpyrifos addition, suggesting that chlorpyrifos did not negatively influence the substrate microbial communities in CWs.

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“…One recently isolated cyanobacterium, Synechocystis sp. StrainPUPCCC 64, was also capable of degrading chlorpyrifos (Singh et al, 2011).…”

Section: Degradation Of Chlorpyrifos By Pure Culturesmentioning

confidence: 99%

Remediation of Chlorpyrifos in Artificially Spiked Soil by Defined Microbial Consortium

Srinivas¹,

Prasad²,

Krishnan³

2016

IJSRES

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Abstract. Excessive use of pesticides as plant protectants has led to the contamination of ecosystem in all parts of the world. This widespread use of pesticides for agricultural purposes has resulted in the persistence of their residues in various environmental matrices, such as soil, water and air. To remove these residues from the environment bioremediation has been proven a suitable technique for reducing pesticide contamination as microorganisms are capable of mineralizing them completely. Chlorpyrifos is a broad spectrum moderately toxic organophosphorous insecticide. It is widely used in agriculture for pest control and in households as a termicide. Long term exposure to chlorpyrifos residues and its metabolites can affect the human health. A microbial consortium that can utilize Chlorpyrifos as a sole source of carbon and energy was isolated from soil through a novel technique involving an initial enrichment in a column reactor followed by enrichment in a shake flask. A diverse range of microorganisms responsible for Chlorpyrifos degradation has been developed. In the present investigation, a new microbial consortium degrading Quinolphos has been developed. Factors such induction, inoculum level, concentration of the substrate, soil pH, soil moisture etc affecting degradation were also studied. Inoculum level of 500µg protein/mL, and a pH 7.5 at ambient temperature (26-28 •C) resulted in degradation of Chlorpyrifos upto 50ppm.

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Chlorpyrifos degradation by the cyanobacterium Synechocystis sp. strain PUPCCC 64

Abstract: Present study reports the biodegradation of chlorpyrifos by Synechocystis sp. Biodegradation of the insecticide by the cyanobacterium is significant as it can be biologically removed from the environment. The cyanobacterium may be used for bioremediation of chlorpyrifos-contaminated soils.

Cited by 104 publications

References 43 publications

Effect of pretilachlor on nitrogen uptake and assimilation by the cyanobacterium Desmonostoc muscorum PUPCCC 405.10

Effect of pretilachlor on nitrogen uptake and assimilation by the cyanobacterium Desmonostoc muscorum PUPCCC 405.10

Mitigation of Wastewater-Borne Chorpyrifos in Constructed Wetlands: an Ecological Suitability Assessment by Macrophyte and Microbial Responses

Remediation of Chlorpyrifos in Artificially Spiked Soil by Defined Microbial Consortium

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