Chlororespiration and Poising of Cyclic Electron Transport

Casano, Leonardo M.; Zapata, José Miguel; Martı́n, Mercedes; Sabater, Bartolomé

doi:10.1074/jbc.275.2.942

Cited by 143 publications

(119 citation statements)

References 31 publications

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“…15,21,[23][24][25] These increases are accompanied by an increase of thylakoid peroxidase but not of SOD, 12,23 in contrast to the response of young leaves in which the three chlororespiratory activities increase in response to different stress situations. By increasing the level of the reduced forms of electron transporters, the increase of Ndh in senescent leaves must increase the rate of the Mehler reaction producing Á O 2 À .…”

Section: Introductionmentioning

confidence: 85%

“…Again, the MDA level was approximately 135 and 85% higher in the Zymographic detection of the NADH dehydrogenase activity of the Ndh complex after native electrophoresis of 120 mg protein solubilized from thylakoid preparations of the fourth leaves of wt and DndhF tobacco 40 days after germination. Details of PCR amplification, the source of the specific antisera, preparation of extracts, electrophoretic and Western-blot methods and zymographic identification/determination are described in previous publications 15,16,20,25 and in Online Supplementary Information second and fourth leaves of DndhF than in the corresponding wt leaves. Even the eighth leaf, that had similar MDA levels in 72-day-old DndhF and wt, showed a small increase of MDA in 79-day-old flowering wt.…”

Section: Introductionmentioning

confidence: 99%

“…[14][15][16] In all, 11 genes (ndh) of plastid DNA encode [17][18][19] components of the Ndh complex which participates in a chlororespiratory electron transport chain 15,20,21 that regulates the redox state of transporters to optimize 22 the rate of cyclic electron transport. In chlororespiration, the Ndh complex provides electrons to plastoquinone, and the Mehler reaction (producing superoxide anion radical, Á O 2 À , which is converted to H 2 O 2 by SOD) and peroxidase/oxidase reactions remove electrons from reduced iron sulfur protein and reduced plastoquinone, respectively.…”

Section: Introductionmentioning

confidence: 99%

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Chloroplasts regulate leaf senescence: delayed senescence in transgenic ndhF-defective tobacco

et al. 2005

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Mitochondrial involvement has not been identified in the programmed cell death (PCD) of leaf senescence which suggests that processes such as those involving reactive oxygen species (ROS) are controlled by chloroplasts. We report that transgenic tobacco (DndhF), with the plastid ndhF gene knocked-out, shows low levels of the plastid Ndh complex, homologous to mitochondrial complex I, and more than a 30-day-delay in leaf senescence with respect to wt. The comparison of activities and protein levels and analyses of genetic and phenotypic traits of wtxDndhF crosses indicate that regulatory roles of mitochondria in animal PCD are assumed by chloroplasts in leaf senescence. The Ndh complex would increase the reduction level of electron transporters and the generation of ROS. Chloroplastic control of leaf senescence provides a nonclassical model of PCD and reveals an unexpected role of the plastid ndh genes that are present in most higher plants.

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Section: Introductionmentioning

confidence: 85%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Chloroplasts regulate leaf senescence: delayed senescence in transgenic ndhF-defective tobacco

et al. 2005

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“…7 indicates the existence in chloroplasts of a propyl gallateinsensitive mechanism for PQ oxidation. Recently, Casano et al (6), studying a reconstituted system containing the Ndh complex and a plastidial hydroquinone peroxidase, proposed the existence of a PQ oxidation pathway using hydrogen peroxide as a terminal acceptor.…”

Section: Measurement Of a Ps Ii-mediated Electron Flow To At-ptox In mentioning

confidence: 99%

“…In higher plants, an involvement of PTOX in PQ oxidation has not been experimentally evidenced. Based on the study of a reconstituted system, Casano et al (6) proposed that a peroxidase using hydrogen peroxide as an electron acceptor may be involved in chlororespiration.…”

mentioning

confidence: 99%

Involvement of a Plastid Terminal Oxidase in Plastoquinone Oxidation as Evidenced by Expression of the Arabidopsis thaliana Enzyme in Tobacco

Joët¹,

Genty²,

Josse³

et al. 2002

Journal of Biological Chemistry

150

156

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Chlororespiration has been defined as a respiratory electron transport chain in interaction with photosynthetic electron transport involving both non-photochemical reduction and oxidation of plastoquinones. Different enzymatic activities, including a plastidencoded NADH dehydrogenase complex, have been reported to be involved in the non-photochemical reduction of plastoquinones. However, the enzyme responsible for plasquinol oxidation has not yet been clearly identified. In order to determine whether the newly discovered plastid oxidase (PTOX) involved in carotenoid biosynthesis acts as a plastoquinol oxidase in higher plant chloroplasts, the Arabidopsis thaliana PTOX gene (At-PTOX) was expressed in tobacco under the control of a strong constitutive promoter. We showed that At-PTOX is functional in tobacco chloroplasts and strongly accelerates the non-photochemical reoxidation of plastoquinols; this effect was inhibited by propyl gallate, a known inhibitor of PTOX. During the dark to light induction phase of photosynthesis at low irradiances, At-PTOX drives significant electron flow to O 2 , thus avoiding over-reduction of plastoquinones, when photosynthetic CO 2 assimilation was not fully induced. We proposed that PTOX, by modulating the redox state of intersystem electron carriers, may participate in the regulation of cyclic electron flow around photosystem I.

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Atmungskomplex in den Chloroplasten löst Alterungsprozesse aus

Krupinska

2005

Biologie in unserer Zeit

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Die Chloroplasten der Blütenpflanzen (Angiospermen) enthalten einen Proteinkomplex (NDH-Komplex), der phylogenetisch und funktionell mit der NADH-Dehydrogenase (Komplex I) der mitochondrialen Atmungskette verwandt ist. Wissenschaftler aus Madrid haben nun herausgefunden, dass der plastidäre Komplex eine Funktion bei der Auslösung von Alterungsprozessen (Seneszenz) hat [5]. ZOOLOG IE | Schnecken in Fesseln gelegt Eine einzigartige Ernährungsstrategie hat die acht Millimeter lange Raupe des erst kürzlich auf Hawaii entdeckten Schmetterlings Hyposmocoma molluscivora entwickelt. Die Raupe fesselt Schnecken mit Hilfe eines Fadens aus ihrer Spinndrüse und verzehrt sie anschließend. Bisher ist keine andere Schmetterlingsraupe bekannt, die sich von Schnecken ernährt.

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Chlororespiration and Poising of Cyclic Electron Transport

Cited by 143 publications

References 31 publications

Chloroplasts regulate leaf senescence: delayed senescence in transgenic ndhF-defective tobacco

Chloroplasts regulate leaf senescence: delayed senescence in transgenic ndhF-defective tobacco

Involvement of a Plastid Terminal Oxidase in Plastoquinone Oxidation as Evidenced by Expression of the Arabidopsis thaliana Enzyme in Tobacco

Atmungskomplex in den Chloroplasten löst Alterungsprozesse aus

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