Chlorophyll breakdown by gamma irradiation in a model system containing linoleic acid

Abstract

The possible breakdown of chlorophyll by irradiation was investigated. Chlorophyll b standard (3 ppm) was added to a methanol solution containing 1% linoleic acid. Irradiation up to 20 kGy was performed with or without N 2 -bubbling, and nonirradiated control was also prepared. Residual chlorophyll b content was analyzed by HPLC and UV-visible spectra after irradiation, and color was measured during 6 h of light exposure (3,300 lux, light intensity) to induce photooxidation. The added chlorophyll b was destroyed by irradiation at 20 kGy with or without N 2 -bubbling. With N 2 -bubbling the oil sample did not develop lipid oxidation during irradiation, and irradiated samples did not develop photooxidation during storage under light. Without N 2 -bubbling, irradiated oil samples had higher PV than nonirradiated samples. The Hunter color a-value of oil increased and the b-value decreased with irradiation at 20 kGy. UV-visible spectra also supported the breakdown of chlorophyll b in solution by irradiation. Irradiation at 2.5 kGy or above destroyed all added chlorophyll b. The results indicate that irradiation technology could be applied to reduce or eliminate the residual chlorophyll in oil processing without developing lipid oxidation during the irradiation process, which would prolong the shelf life of oil products by protecting them from photooxidation during display.Paper no. J9971 in JAOCS 79, 145-150 (February 2002).

Morphological and biochemical responses of Oryza sativa L. (cultivar MR219) to ion beam irradiation

“…This is probably due to a disturbance in chlorophyll b biosynthesis or degradation of its precursors as a result of irradiation (Saha et al, 2010). Current findings were also supported by Byun et al (2002) who discovered the destruction of chlorophyll b by irradiation at 20 kGy.…”

Verification of biological activity of irradiated Sopoongsan, an oriental medicinal prescription, for industrial application of functional cosmetic material

“…Furthermore, Son et al (2001) reported that irradiation can improve the color of natural materials to brighter and transparent such as green tea leaf extract without any changes in its functions. The series of studies using chlorophyll in oil (Byun et al, 2002); persimmon (Disopyros kaki L. folium) leaf and licorice (Glycyrrhiza uralensis Fischer) root (Jo et al, 2003), Lonicera japonica and Curcuma aromatica extracts (Kim et al, 2006) have supported the finding and has been applied in industry. An et al (2005) also reported that irradiation of the polyphenols isolated from green tea leaf up to 40 kGy did not change the physiological activities for human skin.…”

The effects of chronic radiation of gamma ray on protein expression and oxidative stress inBrachypodium distachyon

“…Leaf chlorophyll content is a good indicator of photosynthesis, mutation, and stress, and the eff ect of gamma radiation on chlorophyll content has been investigated (Byun et al 2002, Ling et al 2008. In concordance with our results (Figure 3A), several studies have previously reported that photosynthetic pigments are reduced by gamma-irradiation.…”