Chlorella sp. and Arsenic Compounds: An Attempt to Prepare an Algal Reference Material for Arsenic Compounds

Biotransformation of arsenate to arsenosugars by Chlorella vulgaris

“…This is also reflected by the determination of the standard Fucus certified reference material (IAEA 140), which gave a percentage recovery of 113 ± 28% and the limit of arsenic detection was calculated to be 1.03 µg l −1 . The bioaccumulation results confirm the results of earlier studies by Goessler et al 17 and Maeda. 4 The extraction efficiency of a 1 : 1 (v/v) methanol/water mixture was generally between 11 and 27% of the total arsenic, which is surprisingly low compared with extraction efficiencies for most marine algae 3 ( Table 4).…”

Greedy algae reduce arsenate

“…As reviewed above (Fig. 6), total As cell quotas are higher during the log phase, and As(III) cell quotas are also higher (Goessler et al 1997), suggesting that excretion is by passive diffusion. However, As(III) excretion has been studied in detail for bacteria and fungi, and for these organisms, it is an active transport process (Silver and Phung 1996).…”

Toxicity, biotransformation, and mode of action of arsenic in two freshwater microalgae (Chlorella sp. and Monoraphidium arcuatum)

“…Consequently, C. vulgaris was very tolerant to As(V) (52% growth inhibition at 5 g As[V]/L) when compared to M. arcuatum in our study (IC50: 254 g As[V]/L). Similarly, Chlorella böhm and Chlorella kessleri were tolerant to 2,000 mg As(V)/L, with stationary-phase cell densities 40% higher than controls [30]. These tests used high cell densities (2 ϫ 10 5 cells/ml) and very high phosphate concentrations, 185 mg/L compared to 0.15 mg/L in our study, which would account for the high tolerance observed.…”