CHLAMYDOMONAS <i>SMITHII</i> SP. NOV.–A CHLAMYDOMONAD INTERFERTILE WITH <i>CHLAMYDOMONAS</i> REINHARDTIP

Hoshaw, Robert W.; Ettl, H.

doi:10.1111/j.1529-8817.1966.tb04600.x

Cited by 35 publications

(16 citation statements)

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“…The extensive genetic diversity and cases of known phenotypic variation among isolates (Hoshaw and Ettl, 1966) suggest phenotypic differences are likely among strains of Chlamydomonas. Confocal images among strains do not show any gross morphological differences ( Figure 7A), but we observe some visual differences particularly in cell size.…”

Section: Phenotypic Variation Among Chlamydomonas Strainsmentioning

confidence: 99%

“…At present, there is little information on phenotypic variation among field strains (Hoshaw and Ettl, 1966;Gross et al, 1988), but important indicators such as differences in triacylglycerol accumulation among wild-type strains subjected to stress (Siaut et al, 2011) suggest that Chlamydomonas may harbor intraspecific diversity for important metabolic traits. Although little is known about trait variation at the cellular and molecular level, field isolates of Chlamydomonas offer a potentially large reservoir of functional variation from which recombinant genotypes can be generated for genetic mapping, functional studies, and evolutionary competition and selection experiments.…”

Section: Introductionmentioning

confidence: 99%

“…The genealogy of these strains is fairly well known (Pröschold et al, 2005;Harris, 2008), providing a historical framework for relationships among them. Studies of natural variation in Chlamydomonas further benefit from the isolation and maintenance of field isolates (;30 strains in total) that can be mated and will then produce stable progeny in the laboratory (Hoshaw and Ettl, 1966;Gross et al, 1988;Spanier et al, 1992;Sack et al, 1994;Harris, 2008). With the possible exception of the strain S1 D2 (i.e., CC-2290) (Gross et al, 1988), these environmental isolates are relatively poorly understood.…”

Section: Introductionmentioning

confidence: 99%

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Whole-Genome Resequencing Reveals Extensive Natural Variation in the Model Green Alga Chlamydomonas reinhardtii

et al. 2015

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We performed whole-genome resequencing of 12 field isolates and eight commonly studied laboratory strains of the model organism Chlamydomonas reinhardtii to characterize genomic diversity and provide a resource for studies of natural variation. Our data support previous observations that Chlamydomonas is among the most diverse eukaryotic species. Nucleotide diversity is ;3% and is geographically structured in North America with some evidence of admixture among sampling locales. Examination of predicted loss-of-function mutations in field isolates indicates conservation of genes associated with core cellular functions, while genes in large gene families and poorly characterized genes show a greater incidence of major effect mutations. De novo assembly of unmapped reads recovered genes in the field isolates that are absent from the CC-503 assembly. The laboratory reference strains show a genomic pattern of polymorphism consistent with their origin as the recombinant progeny of a diploid zygospore. Large duplications or amplifications are a prominent feature of laboratory strains and appear to have originated under laboratory culture. Extensive natural variation offers a new source of genetic diversity for studies of Chlamydomonas, including naturally occurring alleles that may prove useful in studies of gene function and the dissection of quantitative genetic traits.

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Section: Phenotypic Variation Among Chlamydomonas Strainsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Whole-Genome Resequencing Reveals Extensive Natural Variation in the Model Green Alga Chlamydomonas reinhardtii

et al. 2015

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“…In this paper we utilize genetic and physical differences in the chloroplast and mitochondrial genomes (14,15) of the interfertile species C. reinhardtii and Chlamydomonas smithii (16) to demonstrate that meiotic zygotes from reciprocal crosses normally transmit the mitochondrial genome of the mt-parent and the chloroplast genome of the mt+ parent to their sexual progeny. In contrast to chloroplast genes (6, 7), UV irradiation of gametes prior to mating has no effect on the paternal transmission of mitochondrial genomes.…”

mentioning

confidence: 99%

Transmission of mitochondrial and chloroplast genomes in crosses of Chlamydomonas.

Boynton¹,

Harris²,

Burkhart³

et al. 1987

Proc. Natl. Acad. Sci. U.S.A.

138

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Physical differences between organelle genomes of the interfertile species Chlamydomonas reinharodt and Chlamydomonas smithii have been used to demonstrate that sexual zygotes transmit chloroplast and mitochondrial DNA from opposite mating types. Processes responsible can be separated functionally and genetically, although both are controlled by mating type. In vegetative diploids, chloroplast and mitochondrial genomes are transmitted biparentally, but a 1-kilobase insert present in the C. smithy mitochondrial genome spreads unidirectionally to all C. reinhardti genomes in a manner reminiscent of the intron found in the mitochondrial 21S rRNA gene of wA strains of yeast.

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“…(Hoshaw and Ettl 1966) have been used in studies of inheritance of both the mitochondrial and chloroplast genomes , and in experiments to map cloned genes in the nuclear genome (Ranum et al 1987;Ranum et al manuscript in preparation).…”

Section: Offprint Requests To P a Lefebvrementioning

confidence: 98%

Extensive restriction fragment length polymorphisms in a new isolate ofChlamydomonas reinhardtii

1988

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A new field isolate of the unicellular green alga Chlamydomonas reinhardtii with useful properties for restriction fragment length polymorphism mapping is described in this report. The isolate, S1-D2 (mating type -), was the only strain found among 24 Chlamydomonas isolates taken from many locations which was interfertile with laboratory strains of C. reinhardtii. It mates at high efficiency, giving tetrads with excellent viability. Using cloned probes for both nuclear and chloroplast genes, we have found numerous restriction fragment length polymorphisms between S1-D2 and laboratory strains of C. reinhardtii.

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CHLAMYDOMONAS SMITHII SP. NOV.–A CHLAMYDOMONAD INTERFERTILE WITH CHLAMYDOMONAS REINHARDTIP

Cited by 35 publications

References 4 publications

Whole-Genome Resequencing Reveals Extensive Natural Variation in the Model Green Alga Chlamydomonas reinhardtii

Whole-Genome Resequencing Reveals Extensive Natural Variation in the Model Green Alga Chlamydomonas reinhardtii

Transmission of mitochondrial and chloroplast genomes in crosses of Chlamydomonas.

Extensive restriction fragment length polymorphisms in a new isolate ofChlamydomonas reinhardtii

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