Chitosan–thioglycolic acid conjugate: An alternative carrier for oral nonviral gene delivery?

Martien, Ronny; Loretz, Brigitta; Thaler, Marlene; Majzoob, Sayeh; Bernkop‐Schnürch, Andreas

doi:10.1002/jbm.a.31135

Cited by 74 publications

(36 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hence, this promising scaffold was utilised for NP preparation for the purpose of oral controlled delivery of drugs and macromolecules. Thiolated CS-NPs made from thioglycolic acid and CS were first prepared to deliver DNA to the cells via the oral route (109). The DNA thiolated CS-NPs showed significant improvement in transfection rate when compared to DNA encapsulated unmodified CS-NPs.…”

Section: Thiolated Chitosanmentioning

confidence: 99%

Recent Advancement of Chitosan-Based Nanoparticles for Oral Controlled Delivery of Insulin and Other Therapeutic Agents

2010

View full text Add to dashboard Cite

Abstract. Nanoparticles composed of naturally occurring biodegradable polymers have emerged as potential carriers of various therapeutic agents for controlled drug delivery through the oral route. Chitosan, a cationic polysaccharide, is one of such biodegradable polymers, which has been extensively exploited for the preparation of nanoparticles for oral controlled delivery of several therapeutic agents. In recent years, the area of focus has shifted from chitosan to chitosan derivatized polymers for the preparation of oral nanoparticles due to its vastly improved properties, such as better drug retention capability, improved permeation, enhanced mucoadhesion and sustained release of therapeutic agents. Chitosan derivatized polymers are primarily the quaternized chitosan derivatives, chitosan cyclodextrin complexes, thiolated chitosan, pegylated chitosan and chitosan combined with other peptides. The current review focuses on the recent advancements in the field of oral controlled release via chitosan nanoparticles and discusses about its in vitro and in vivo implications.

show abstract

Section: Thiolated Chitosanmentioning

confidence: 99%

Recent Advancement of Chitosan-Based Nanoparticles for Oral Controlled Delivery of Insulin and Other Therapeutic Agents

2010

View full text Add to dashboard Cite

show abstract

“…Chitosan modified with poly(d,llactide-co-glycolide) (PLGA) nanosphere provided a platform for prolong and sustained intracellular release of nucleic acids [66]. Introduction of thioglycolic acid (TGA) to chitosan improved the efficacy of oral gene delivery systems due to increased stability from nuclease degradation [67]. Thiolated chitosan appeared to possess enhanced mucoadhesiveness and cell penetration, higher gene transfer potential and sustained gene expression [68].…”

Section: Chitosan Derivativesmentioning

confidence: 99%

Chitosan and its derivatives for gene delivery

Saranya

Moorthi

Sekaran

et al. 2011

International Journal of Biological Macromolecules

227

102

View full text Add to dashboard Cite

“…Polyplexes, complexes of DNA and polymer, are one example of delivery systems that offer advantages such as high stability and high transfection efficiencies [56]. Regarding thiolated chitosans, it was demonstrated that the complexation of 102 F. Sarti and A. Bernkop-Schn€ urch chitosan-TGA with pDNA leads to a transfection rate in Caco-2 cells that was fivefold higher than that gained with unmodified chitosan/pDNA [57]. Thiolated chitosan/pDNA complexes were prepared at pH 4.0 and 5.0 and were resistant to degradation during incubation with DNase I for 30 min.…”

Section: Transfection-enhancing Propertiesmentioning

confidence: 99%

Chitosan and Thiolated Chitosan

Sarti

Bernkop‐Schnürch

2011

Advances in Polymer Science

Self Cite

View full text Add to dashboard Cite

Thiolated chitosans constitute an integral part of designated "thiomers", which are thiolated polymers widely investigated for non-invasive drug delivery. In brief, thiomers display thiol-group-bearing ligands on their polymer backbone. Through thiol/disulfide exchange reactions and/or a simple oxidation process, disulfide bonds are formed between such polymers and the cysteine-rich subdomains of mucus glycoproteins, thus building up the mucus gel layer. Most chemical modifications of chitosan are performed at the free amino groups of the glucosamine units. So far, the alkyl thiomers chitosan-cysteine, chitosan-thiobutylamidine, chitosan-thioglycolic acid, chitosan-N-acetylcysteine, and chitosan-thioethylamidine and the aryl thiomers chitosan-6-mercaptonicotinic acid and chitosan-4-mercaptobenzoic acid have been generated. Due to the immobilization of thiol groups on the chitosan backbone, its mucoadhesive, permeation enhancing, in situ gelling, efflux pump inhibitory, and controlled drug release properties are improved. The great benefits of this new generation of chitosans in comparison to the corresponding unmodified polymers has been verified via numerous in vivo studies on various mucosal membranes. A proof of concept for oral, nasal and buccal drug delivery is provided. This chapter includes an overview of the mechanism of adhesion and the design of thiomers as well as of delivery systems comprising thiolated chitosans and their in vivo performance.

show abstract

Chitosan–thioglycolic acid conjugate: An alternative carrier for oral nonviral gene delivery?

Cited by 74 publications

References 34 publications

Recent Advancement of Chitosan-Based Nanoparticles for Oral Controlled Delivery of Insulin and Other Therapeutic Agents

Recent Advancement of Chitosan-Based Nanoparticles for Oral Controlled Delivery of Insulin and Other Therapeutic Agents

Chitosan and its derivatives for gene delivery

Chitosan and Thiolated Chitosan

Contact Info

Product

Resources

About