Chitosan stabilized nasal emulsion delivery system for effective humoral and cellular response against recombinant tetravalent dengue antigen

Vemireddy, Sravanthi; Pallavi, Madhurantakam C. Preethi; Halmuthur, M. Sampath Kumar

doi:10.1016/j.carbpol.2018.02.073

Cited by 16 publications

(11 citation statements)

References 36 publications

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“…2,4 Because nanoparticles form stable nanoemulsion particles before water dilution, the subsequently added antigen mainly exists in the bulk water, and can, therefore, be freely transported and adsorbed on the surface of nanoparticles, which o u p 7 1 4 2 1 2 8 3 5 4 2 4 9 5 6 6 3 Saline 0±0 5±2 8±5 7±5 5±2 6±2 6±5 6±5 4±0 Vaccine 0±0 33±28 70±53 525±340 934±535 525±340 262±170 288±219 198±195 Al(OH)3+Vaccine 0±0 115±129 352±290 1331±687 2662±3123 2355±2220 1894±1444 1433±561 973±687 D1+Vaccine 0±0 1638±561 2867±1122 6144±2896 4608±3510 4096±2508 3584±1145 2252±1122 1433±561 D2+Vaccine 0±0 1945±1374 2662±1374 6553±2243 5734±2243 6553±2243 5324±2748 4096±0 1945±1275 D3+Vaccine 0±0 1740±1430 2252±1122 4505±2243 5324±2748 5324±2748 2867±1122 2662±916 1792±572 D4+Vaccine 0±0 333±172 563±280 4096±2401 5734±2243 4506±2243 3686±2670 3379±3063 can easily be identified by antigen-presenting cells, thus helping to stimulate antibody production. 25,26 In the capsule mode, however, the antigen solution is firstly added to form a coarse emulsion. And finally, be exposed to the bulk water and never adsorbed to the nanoparticle surface, so it causes nearly the same level of the immune response as none adjuvant group.…”

Section: Discussionmentioning

confidence: 99%

<p>O/W Nanoemulsion as an Adjuvant for an Inactivated H3N2 Influenza Vaccine: Based on Particle Properties and Mode of Carrying</p>

Zhao¹,

Zhu²,

Ying-bo³

2020

IJN

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Background and Purpose: Adjuvant can reduce vaccine dosage and acquire better immune protection to the body, which helps to deal with the frequent outbreaks of influenza. Nanoemulsion adjuvants have been proved efficient, but the relationship between their key properties and the controlled release which greatly affects immune response is still unclear. The present work explores the role of factors such as particle size, the polydispersity index (PDI), stability and the safety of nanoemulsions by optimizing the water concentration, oil phase and modes of carrying, to explain the impact of those key factors above on adjuvant effect. Methods: Isopropyl myristate (IPM), white oil, soybean oil, and grape-kernel oil were chosen as the oil phase to explore their roles in emulsion characteristics and the adjuvant effect. ICR mice were immunized with an emulsion-inactivated H3N2 split influenza vaccine mixture, to compare the nanoemulsion's adjuvant with traditional aluminium hydroxide or complete Freund's adjuvant. Results: Particle size of all the nanoemulsion formed in our experiment ranged from 20 nm to 200 nm and did not change much when diluted with water, while the PDI decreased obviously, indicating that the particles tended to become more dispersive. Formulas with 80% or 85.6% water concentration showed significant higher HAI titer than aluminium hydroxide or complete Freund's adjuvant, and adsorption rather than capsule mode showed higher antigen delivery efficiency. As mentioned about oil phase, G (IPM), F (white oil), H (soybean oil), and I (grape-kernel oil) showed a decreasing trend in their adjuvant efficiency, and nanoemulsion G was the best adjuvant with smaller and uniform particle size. Conclusion: Emulsions with a smaller, uniform particle size had a better adjuvant effect, and the adsorption mode was generally more efficient than the capsule mode. The potential adjuvant order of the different oils was as follows: IPM > white oil > soybean oil > grape-kernel oil.

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Section: Discussionmentioning

confidence: 99%

<p>O/W Nanoemulsion as an Adjuvant for an Inactivated H3N2 Influenza Vaccine: Based on Particle Properties and Mode of Carrying</p>

Zhao¹,

Zhu²,

Ying-bo³

2020

IJN

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“…The plates were coated with antigen, blocked with 1% BSA, addition of serially diluted serum sample tagged with 1:3000 diluted anti-mouse IgG1 horseradish peroxidase conjugates and anti-mouse IgG2a horseradish peroxidase conjugates. Color was developed with TMB and absorbance was determined opting same Tecan multimode reader at 450nm [7].…”

Section: Estimation Of Igg1 and Igg2a Subtypesmentioning

confidence: 99%

Immunopharmacological evaluation of adjuvant efficacy of Monophosphoryl lipid-A and CpG ODN with SARS-CoV-2 RBD antigen

Sambyal

Vemireddy²,

Khatri

et al. 2022

Preprint

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SARS-CoV-2 infection has made the mankind to witness most sever and serious pandemic situation in the history. Millions of people have suffered and are still suffering with this infection which has caused a mass mortality in the past three years. Development of an effective vaccine to control the spread of infection and to prevent this viral infection is need of the hour. Adjuvanted vaccines have proven their efficacy in controlling many other viral infections like flu, keeping this context in view we have evaluated the immunopharmacological efficacy of two adjuvants MPL-A and CpG ODN in combination with MF59 emulsion against SARS-CoV-2 antigen. From the data obtained we can infer that both the adjuvants were capable of eliciting a potent antibody response against antigen alone and MF59 groups. Comparatively MPL-A was eliciting a Th1 polarized response in terms of IgG2a and cytokine production. Both the adjuvants were capable of enhancing the CD 4, 8 and 19 cell populations. Overall the pre clinical evaluation has given a clue of the effectiveness of MPL-A and CpG adjuvants against SARS-CoV-2 antigen.

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Section: Properties Of Chitosan and Its Derivativesmentioning

confidence: 99%

“…The immunostimulatory effect of chitosan was also determined by an evaluation of cytokines, in which the emulsion with recombinant tetravalent dengue antigen containing chitosan as a stabilizer had a good ability to activate IL-12 and IL-1β compared to the control sample. Therefore, the action of stimulating the immunity of the emulsion with chitosan was confirmed, in addition to the release of the antigen [ 69 ].…”

Section: Properties Of Chitosan and Its Derivativesmentioning

confidence: 99%

“…Due to the interaction between the positive groups of chitosan at pH > 6.5 and the hydrophobic groups of oleic acid, chitosan has been used by researchers to stabilize a nasal W/O emulsion of oleic acid. The stability of the system is also based on electrostatic interactions between the protonated amino groups of chitosan and the carboxyl groups of oleic acid [69]. Schiff Base: hydrogel that prevents postoperative adhesion [71] Studies have shown that chitosan coating influences the adsorption and in vivo resistance of liposomes, resulting in superior properties compared to uncoated liposomes in terms of drug delivery.…”

Section: Lipidsmentioning

confidence: 99%

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New Opportunity to Formulate Intranasal Vaccines and Drug Delivery Systems Based on Chitosan

Popescu

Ghica

Dinu-Pîrvu

et al. 2020

IJMS

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In an attempt to develop drug delivery systems that bypass the blood–brain barrier (BBB) and prevent liver and intestinal degradation, it was concluded that nasal medication meets these criteria and can be used for drugs that have these drawbacks. The aim of this review is to present the influence of the properties of chitosan and its derivatives (mucoadhesion, permeability enhancement, surface tension, and zeta potential) on the development of suitable nasal drug delivery systems and on the nasal bioavailability of various active pharmaceutical ingredients. Interactions between chitosan and proteins, lipids, antigens, and other molecules lead to complexes that have their own applications or to changing characteristics of the substances involved in the bond (conformational changes, increased stability or solubility, etc.). Chitosan and its derivatives have their own actions (antibacterial, antifungal, immunostimulant, antioxidant, etc.) and can be used as such or in combination with other molecules from the same class to achieve a synergistic effect. The applicability of the properties is set out in the second part of the paper, where nasal formulations based on chitosan are described (vaccines, hydrogels, nanoparticles, nanostructured lipid carriers (NLC), powders, emulsions, etc.).

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Chitosan stabilized nasal emulsion delivery system for effective humoral and cellular response against recombinant tetravalent dengue antigen

Cited by 16 publications

References 36 publications

<p>O/W Nanoemulsion as an Adjuvant for an Inactivated H3N2 Influenza Vaccine: Based on Particle Properties and Mode of Carrying</p>

<p>O/W Nanoemulsion as an Adjuvant for an Inactivated H3N2 Influenza Vaccine: Based on Particle Properties and Mode of Carrying</p>

Immunopharmacological evaluation of adjuvant efficacy of Monophosphoryl lipid-A and CpG ODN with SARS-CoV-2 RBD antigen

New Opportunity to Formulate Intranasal Vaccines and Drug Delivery Systems Based on Chitosan

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