Chitosan/silk fibroin-based, Schwann cell-derived extracellular matrix-modified scaffolds for bridging rat sciatic nerve gaps

Gu, Yun; Zhu, Jianbin; Xue, Cong; Li, Zhenmeiyu; Ding, Fei; Yang, Yumin; Gu, Xiaosong

doi:10.1016/j.biomaterials.2013.11.087

Cited by 215 publications

(143 citation statements)

References 45 publications

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“…Our results demonstrated that paracrine factors released from SHEDs not only promoted angiogenesis directly but also enhanced the potential for angiogenesis in SCs. ECM consists of collagens, adhesive glycoproteins, and proteoglycans produced by resident cells in tissues or organs [66]. Cell adhesion to ECM enhances survival, differentiation, and migration [67].…”

Section: Discussionmentioning

confidence: 99%

Peripheral Nerve Regeneration by Secretomes of Stem Cells from Human Exfoliated Deciduous Teeth

Sugimura‐Wakayama

Katagiri

Osugi

et al. 2015

Stem Cells and Development

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Peripheral nerve regeneration across nerve gaps is often suboptimal, with poor functional recovery. Stem cell transplantation-based regenerative therapy is a promising approach for axon regeneration and functional recovery of peripheral nerve injury; however, the mechanisms remain controversial and unclear. Recent studies suggest that transplanted stem cells promote tissue regeneration through a paracrine mechanism. We investigated the effects of conditioned media derived from stem cells from human exfoliated deciduous teeth (SHED-CM) on peripheral nerve regeneration. In vitro, SHED-CM-treated Schwann cells exhibited significantly increased proliferation, migration, and the expression of neuron-, extracellular matrix (ECM)-, and angiogenesis-related genes. SHED-CM stimulated neuritogenesis of dorsal root ganglia and increased cell viability. Similarly, SHED-CM enhanced tube formation in an angiogenesis assay. In vivo, a 10-mm rat sciatic nerve gap model was bridged by silicon conduits containing SHED-CM or serum-free Dulbecco's modified Eagle's medium. Light and electron microscopy confirmed that the number of myelinated axons and axon-to-fiber ratio (G-ratio) were significantly higher in the SHED-CM group at 12 weeks after nerve transection surgery. The sciatic functional index (SFI) and gastrocnemius (target muscle) wet weight ratio demonstrated functional recovery. Increased compound muscle action potentials and increased SFI in the SHED-CM group suggested sciatic nerve reinnervation of the target muscle and improved functional recovery. We also observed reduced muscle atrophy in the SHED-CM group. Thus, SHEDs may secrete various trophic factors that enhance peripheral nerve regeneration through multiple mechanisms. SHED-CM may therefore provide a novel therapy that creates a more desirable extracellular microenvironment for peripheral nerve regeneration.

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Section: Discussionmentioning

confidence: 99%

Peripheral Nerve Regeneration by Secretomes of Stem Cells from Human Exfoliated Deciduous Teeth

Sugimura‐Wakayama

Katagiri

Osugi

et al. 2015

Stem Cells and Development

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“…It has been well documented that an intimate relationship exists between neurons and glia, both during development and in response to injury [25]. Schwann cells (SCs), the major glial cells of the peripheral nervous system, can facilitate organization of the neural ECM, form axon myelination, and provide structural and trophic support for axonal regrowth [12,26]. Hence, an investigation of the reshaping effect on the fibrin gels caused by SCs differentiated from EMSCs is of great significance for the development of an appropriate scaffold for nerve tissue regeneration.…”

Section: Introductionmentioning

confidence: 99%

“…in the desired direction [9e11]. However, this investigational approach has stagnated at the laboratory stage due to various challenges, such as intrinsic flaws in the materials, the difficulty in delivering factors, poor cell phenotypic stability, long treatment duration and high cost [12,13]. Therefore, these limitations have encouraged the development of an alternative approach that relies on extracellular matrix (ECM).…”

Section: Introductionmentioning

confidence: 99%

Nasal ectomesenchymal stem cells: Multi-lineage differentiation and transformation effects on fibrin gels

Zhang

Deng

et al. 2015

Biomaterials

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“…In the architecture of scafold used for tissue regeneration, the choice of biomaterial is of critical importance. The variety of processes used in the manufacture of scafolds modifying the surface and bulk properties inluences both the architectural and the similarity of the scafold with the native organic tissues, and may actively provide bioactive cues to the residing cells for regulations of the activities [50,51].…”

Section: Tissue Regeneration: a Biomimetic Designmentioning

confidence: 99%

Three-Dimensional and Biomimetic Technology in Cardiac Injury After Myocardial Infarction: Effect of Acellular Devices on Ventricular Function and Cardiac Remodelling

Chaud¹,

Alves²,

Rebelo³

et al. 2017

Scaffolds in Tissue Engineering - Materials, Technologies and Clinical Applications

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Dilated cardiomyopathy (DMC) of ischemic or non-ischemic aetiology remains a lethal condition nowadays. Despite early percutaneous or medical revascularization after an acute myocardial infarct (AMI), many patients still develop DMC and severe heart failure due to cardiac remodelling. Possibility of regenerating myocardium already damaged or at least inducing a more positive cardiac remodelling with use of biodegradable scafolds has been atempted in many experimental studies, which can be cellular or acellular. In the cellular scafolds, the cells are incorporated in the structure prior to implantation of the same into the injured tissue. Acellular scafolds, in turn, are composites that use one or more biomaterials present in the extracellular matrix (ECM), such as proteoglycans non-proteoglycan polysaccharide, proteins and glycoproteins to stimulate the chemotaxis of cellular/molecular complexes as growth factors to initiate speciic regeneration. For the development of scafold, the choice of biomaterials to be used must meet speciic biological, chemical and architectural requirements like ECM of the tissue of interest. In acute myocardial infarction, treating the root of the problem by repairing injured tissue is more beneicial to the patient. Inducing more constructive forms of endogenous repair. Thus, patches of acellular scafolds capable of mimicking the epicardium and ECM should be able to atenuate both cardiac remodelling and adverse cardiac dysfunction.

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Chitosan/silk fibroin-based, Schwann cell-derived extracellular matrix-modified scaffolds for bridging rat sciatic nerve gaps

Cited by 215 publications

References 45 publications

Peripheral Nerve Regeneration by Secretomes of Stem Cells from Human Exfoliated Deciduous Teeth

Peripheral Nerve Regeneration by Secretomes of Stem Cells from Human Exfoliated Deciduous Teeth

Nasal ectomesenchymal stem cells: Multi-lineage differentiation and transformation effects on fibrin gels

Three-Dimensional and Biomimetic Technology in Cardiac Injury After Myocardial Infarction: Effect of Acellular Devices on Ventricular Function and Cardiac Remodelling

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