Chitosan microparticles for mucosal vaccination against diphtheria: oral and nasal efficacy studies in mice

Lubben, I.M. van der; Kersten, Gideon; Fretz, Marjan M.; Beuvery, Coen; Verhoef, J.; Junginger, Hans E.

doi:10.1016/s0264-410x(02)00686-2

Cited by 211 publications

(100 citation statements)

References 24 publications

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“…Several studies have shown that the uptake by M-cells was significantly enhanced and degradation of protein and peptide vaccines in the gastrointestinal track was prevented after the incorporation of vaccine with CMs. 59,[68][69][70][71] Due to its nontoxicity and potent antigen binding properties, chitosan has been considered a promising tool for oral vaccination.…”

Section: Oral Deliverymentioning

confidence: 99%

“…Extensive research on CMs for mucosal vaccine delivery, in particular, the uptake of CMs in murine Peyer's patches in vitro and in vivo, was carried out by van der Lubben et al 59,68,70,71 They prepared a human intestinal M-cell model by coculturing Caco-2 and Raji-cells and investigated the uptake of CMs. 59 No morphological changes in the monolayer were observed and this model was used to examine the in vitro uptake of CMs for oral vaccine delivery.…”

mentioning

confidence: 99%

“…A diphtheria toxoid (DT) was used to examine the enhancement of both systemic and local immune responses. 70 Unloaded CMs, DT-loaded CMs, and DT in phosphate-buffered saline (PBS) were delivered into mice by oral and nasal administration. DT associated with alum was subcutaneously immunized in mice as a positive control.…”

mentioning

confidence: 99%

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Design and application of chitosan microspheres as oral and nasal vaccine carriers: an updated review

Islam¹,

Firdous²,

Choi³

et al. 2012

IJN

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Chitosan, a natural biodegradable polymer, is of great interest in biomedical research due to its excellent properties including bioavailability, nontoxicity, high charge density, and mucoadhesivity, which creates immense potential for various pharmaceutical applications. It has gelling properties when it interacts with counterions such as sulfates or polyphosphates and when it crosslinks with glutaraldehyde. This characteristic facilitates its usefulness in the coating or entrapment of biochemicals, drugs, antigenic molecules as a vaccine candidate, and microorganisms. Therefore, chitosan together with the advance of nanotechnology can be effectively applied as a carrier system for vaccine delivery. In fact, chitosan microspheres have been studied as a promising carrier system for mucosal vaccination, especially via the oral and nasal route to induce enhanced immune responses. Moreover, the thiolated form of chitosan is of considerable interest due to its improved mucoadhesivity, permeability, stability, and controlled/extended release profile. This review describes the various methods used to design and synthesize chitosan microspheres and recent updates on their potential applications for oral and nasal delivery of vaccines. The potential use of thiolated chitosan microspheres as next-generation mucosal vaccine carriers is also discussed.

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Section: Oral Deliverymentioning

confidence: 99%

mentioning

confidence: 99%

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Design and application of chitosan microspheres as oral and nasal vaccine carriers: an updated review

Islam¹,

Firdous²,

Choi³

et al. 2012

IJN

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show abstract

“…Changes in charge density have an effect on the dissolution and binding properties of chitosan (Domard, 1996). The degree of deacetylation also controls the degree of crystallinity and hydrophobicity of chitosan (Vander Lubben et al, 2003). Chitosan enhances the flocculation of sludge, and the flocculation efficiency depends on both DD and molecular weight (MW).…”

Section: Fig 1 Deacetylation Of Chitin To Chitosanmentioning

confidence: 99%

Enhancing Biogas Production and UASB Start-Up by Chitosan Addition

Phalakornkule¹,

Khemkhao²

2012

Biogas

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“…Nanoparticles made up of biodegradable and biocompatible polymers such as poly (D, L-lactide-co-glycolic acid) (PLGA) (Spiers et al, 2000;Kavanagh et al, 2003;Jiang et al, 2005), starch (Wikingsson & Sjoholm, 2002), and chitosan (McNeela et al, 2000;Van der Lubben et al, 2003) have been extensively studied. Significant advantages of these biodegradable polymers are their history of safe use, proven biocompatibility, and ability to control the time and rate of polymer degradation and protein/ antigen release, thus increasing the half-life of bioactives (Kersten & Hirshberg, 2004).…”

Section: Nanoparticles As Protein Stabilizermentioning

confidence: 99%