Chitosan Lactate Particles for Non-Compression Hemostasis on Hepatic Resection

Jiang, Yuhui; Tang, Xiaoxuan; Li, Tao; Ling, Jue; Ge, Yifan; Yang, Yumin

doi:10.3390/polym15030656

Cited by 2 publications

(4 citation statements)

References 30 publications

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“…However, significant bleeding was observed in the control group by 180 s. And a large amount of blood was observed to soak the gauze in the control group with femoral artery hemorrhage. In addition, we further supplement an experiment by using a 1/3 liver defect hemorrhage model, which is known as strict bleeding model [ 32,33 ] (Figure S5, Supporting Information). Untreated group and Alg sponges group were used as controls in the liver hemorrhage model.…”

Section: Resultsmentioning

confidence: 99%

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Multiple Non‐Covalent Cross‐Linked Multifunctional Strong Hemostatic Agent for Dynamic Exposure Hemostasis

Ji,

Li,

Hou

et al. 2023

Adv Healthcare Materials

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Trauma requires immediate hemostasis during primary care, as well as durable hemostasis that can withstand dynamic wound exposure. Although current hemostatic materials can treat bleeding sites in emergency situations, their mechanical strength and storage conditions limit their practical application. The simultaneous combination of good mechanical properties, storage stability, biocompatibility, and rapid hemostasis of hemostatic materials remains a challenge. In this paper, a novel hemostatic material based on multiple non‐covalent bond crosslinking, which has excellent mechanical properties, good biocompatibility, storage stability, and rapid hemostasis ability, is reported. Under the drive of multiple non‐covalent bonds, the flowability of hydrogel micro‐modules (HM) decreases rapidly within 20 s after exposure to physiological saline. The HM form a gel barrier with a tensile strength of 62.10 kPa and an elongation at break of 1976% under multiple non‐covalent bonding. Furthermore, the mechanical properties do not change significantly after 30 days of storage. Cell viability is maintained at over 80% after 3 days of incubation with the cells, and the hemolysis test shows a very low hemolysis rate (2.08%). The hemostatic gel formed by HM effectively prevents secondary bleeding in dynamic hemostasis experiments simulating transportation. This work provides a hemostatic material with comprehensive properties for practical applications.

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Section: Resultsmentioning

confidence: 99%

“…In addition, we further supplement an experiment by using a 1/3 liver defect hemorrhage model, which is known as strict bleeding model [32,33] (Figure S5, Supporting Information). Untreated group and Alg sponges group were used as controls in the liver hemorrhage model.…”

Section: Dynamic Hemostasis In Vivomentioning

confidence: 99%

Multiple Non‐Covalent Cross‐Linked Multifunctional Strong Hemostatic Agent for Dynamic Exposure Hemostasis

Ji,

Li,

Hou

et al. 2023

Adv Healthcare Materials

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“…Total blood volume (TBV) was used to calculate the percentage of blood loss. The total blood volume of the rabbit was assumed to be 60 mL/kg . One animal from each group was sacrificed, and the liver, kidney, heart, and spleen were isolated for ex vivo imaging under the IVIS system.…”

Section: Methodsmentioning

confidence: 99%

“…The total blood volume of the rabbit was assumed to be 60 mL/kg. 30 One animal from each group was sacrificed, and the liver, kidney, heart, and spleen were isolated for ex vivo imaging under the IVIS system. For the rest of the animals in each group, survival was monitored for 3 weeks.…”

Section: Percentage Drug Loading and Entrapment Efficiencymentioning

confidence: 99%

Spatiotemporally Controlled Release of Etamsylate from Bioinspired Peptide-Functionalized Nanoparticles Arrests Bleeding Rapidly and Improves Clot Stability in a Rabbit Internal Hemorrhage Model

Mukherjee,

Sasmal,

Reddy

et al. 2024

ACS Biomater. Sci. Eng.

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Achieving rapid clotting and clot stability are important unmet goals of clinical management of noncompressible hemorrhage. This study reports the development of a spatiotemporally controlled release system of an antihemorrhagic drug, etamsylate, in the management of internal hemorrhage. Gly-Arg-Gly-Asp-Ser (GRGDS) peptidefunctionalized chitosan nanoparticles, with high affinity to bind with the GPIIa/IIIb receptor of activated platelets, were loaded with the drug etamsylate (etamsylate-loaded GRGDS peptide-functionalized chitosan nanoparticles; EGCSNP). Peptide conjugation was confirmed by LCMS, and the delivery system was characterized by DLS, SEM, XRD, and FTIR. In vitro study exhibited 90% drug release till 48 h fitting into the Weibull model. Plasma recalcification time and prothrombin time tests of GRGDS-functionalized nanoparticles proved that clot formation was 1.5 times faster than nonfunctionalized chitosan nanoparticles. The whole blood clotting time was increased by 2.5 times over clot formed under nonfunctionalized chitosan nanoparticles. Furthermore, the application of rheometric analysis revealed a 1.2 times stiffer clot over chitosan nanoparticles. In an in vivo liver laceration rabbit model, EGCSNP spatially localized at the internal injury site within 5 min of intravenous administration, and no rebleeding was recorded up to 3 h. The animals survived for 3 weeks after the injury, indicating the strong potential of the system for the management of noncompressible hemorrhage.

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Chitosan Lactate Particles for Non-Compression Hemostasis on Hepatic Resection

Cited by 2 publications

References 30 publications

Multiple Non‐Covalent Cross‐Linked Multifunctional Strong Hemostatic Agent for Dynamic Exposure Hemostasis

Multiple Non‐Covalent Cross‐Linked Multifunctional Strong Hemostatic Agent for Dynamic Exposure Hemostasis

Spatiotemporally Controlled Release of Etamsylate from Bioinspired Peptide-Functionalized Nanoparticles Arrests Bleeding Rapidly and Improves Clot Stability in a Rabbit Internal Hemorrhage Model

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