Chitosan kills Escherichia coli through damage to be of cell membrane mechanism

Li, Xiaofang; Feng, Xin; Yang, Sheng; Fu, Guoqing; Ting-pu, Wang; Su, Zhong-Xing

doi:10.1016/j.carbpol.2009.07.011

Cited by 179 publications

(93 citation statements)

References 30 publications

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“…In general, chitosan has been reported to show strong inhibition towards E. coli, but differs with several factors such as molecular weight. Previous study on the bactericidal activity of chitosan against E. coli has shown that chitosan caused lysis and cytoplasmic membrane disruption in E. coli observed via ultra-structural analysis using TEM 35 .The similar study also suggested that the electrostatic interaction between -NH 3 + groups of chitosan and the negatively charged phospholipids component (carbonyl and phosphoryl groups) of the cell membrane eventually brought about damage to the bacterial cell membrane 36 . Other study has also reported that the release of protonate glucosamine (due to the protonation of amine group) from the chitosan film could contribute to the inhibition of bacterial growth 37 .…”

Section: Antibacterial Studysupporting

confidence: 51%

Composite Film of Chitosan Loaded Norfloxacin With Improved Flexibility and Antibacterial Activity for Wound Dressing Application

Sebri¹,

Amin²

2017

Orient. J. Chem

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This research develops chitosan film incorporated norfloxacin (CH-NOR) designed to combat the infection of bacteria around the wound area. Inclusion of norfloxacin increased the strain-atbreak or flexibility of the film. Swelling behavior and water vapor transmission rates (WVTRs) values are improved and optimum for CH-NOR1.0 film at 524 ± 65 % and 1916 ± 78 g m -2 d -1 , respectively. In-vitro cell studies show that the present of norfloxacin in CH film gradually decreased the number of human dermal fibroblast cell (CRL2522, ATCC) after incubated for 72 hours. In-contrast, antibacterial activities of CH-NOR films exhibit strong inhibition zones against Gram-positive (Staphylococcus aureus and Bacillus cereus) and Gram-negative (Escherichia coli and Klebsiella pneumoniae) bacteria.

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Section: Antibacterial Studysupporting

confidence: 51%

Composite Film of Chitosan Loaded Norfloxacin With Improved Flexibility and Antibacterial Activity for Wound Dressing Application

Sebri¹,

Amin²

2017

Orient. J. Chem

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“…Exposure to antibacterial agents usually changes the bacterial cell membrane integrity and permeability [22]. Therefore, leakage of intracellular components is a classic indication of membrane damage.…”

Section: Discussionmentioning

confidence: 99%

Chitosan Silver Nano Composites (Cagncs) as Potential Antibacterial Agent to Control Vibrio tapetis

Shs

Gi²,

Rgpt³

et al. 2014

J Veterinar Sci Technol

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Nanocomposites exhibit high performance on antimicrobial activities and have the potential to be developed alternative antibiotics. In this study, antibacterial effects of chitosan silver nano composites (CAgNCs) were investigated using pathogenic Vibrio tapetis as a bacterial model. Agar disc diffusion and turbidimetric assay results showed that CAgNCs could inhibit the growth of V. tapetis in concentration depended manner. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of CAgNCs against V. tapetis were 50 μg/mL and 100 μg/mL, respectively. The field emission scanning electron microscopy (FE-SEM) images analysis of V. tapetis showed severe structural damage to cells after treating CAgNCs at 75 μg/mL compared to un-treated bacteria. Moreover, CAgNCs induce the intracellular ROS level, leakage of nucleic materials (RNA and DNA), decrease the cell viability and protein level in V. tapetis cells. Herein, we demonstrate that CAgNCs as effective antibacterial agent with capability to disrupt cell membrane, de-stabilize the membrane permeability, induce the oxidative stress and inhibit the expression of protein or synthesis of macromolecules. Overall results from this study suggest that mode of action of CAgNCs may be associated with excessive generation of ROS, loss in membrane integrity and inhibiting protein synthesis that cause the bacterial cell death.

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“…Bacteria for TEM samples were prepared as previously described (12). Overnight cultures of E. coli or S. aureus in MHB were adjusted to 10 8 CFU/ml, and aliquots were added to CD hydrogel, DA, and Triton X-100 in separate experiments.…”

Section: Methodsmentioning

confidence: 99%

Antimicrobial Properties of a Chitosan Dextran-Based Hydrogel for Surgical Use

Aziz

Cabral

Brooks

et al. 2012

Antimicrob Agents Chemother

127

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A chitosan dextran-based (CD) hydrogel, developed for use in endoscopic sinus surgery, was tested for antimicrobial activity in vitro against a range of pathogenic microorganisms. The microdilution technique was used to determine minimum inhibitory, minimum bactericidal, and minimum fungicidal concentrations. In addition, the time-kill efficacy of CD hydrogel was determined for two bacterial species. Scanning and transmission electron microscopy were carried out to elucidate the antimicrobial mechanism of this compound. CD hydrogel was found to be effective against Staphylococcus aureus, Streptococcus pyogenes, Escherichia coli, and Clostridium perfringens at its surgical concentration of 50,000 mg/liter. Minimum bactericidal concentrations ranged from 2,000 to 50,000 mg/liter. Dextran aldehyde (DA) was found to be the antimicrobial component of the CD hydrogel with MBC ranging from 2,000 to 32,000 mg/liter. S. aureus appeared to be killed at a slightly faster rate than E. coli. Candida albicans and Pseudomonas aeruginosa were more resistant to CD hydrogel and DA. Scanning and transmission electron microscopy of E. coli and S. aureus incubated with CD hydrogel and DA alone revealed morphological damage, disrupted cell walls, and loss of cytosolic contents, compatible with the proposed mode of action involving binding to cell wall proteins and disruption of peptide bonds. Motility and chemotaxis tests showed E. coli to be inhibited when incubated with DA. The antibacterial activity of CD hydrogel may make it a useful postsurgical aid at other body sites, especially where there is a risk of Grampositive infections.

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Chitosan kills Escherichia coli through damage to be of cell membrane mechanism

Cited by 179 publications

References 30 publications

Composite Film of Chitosan Loaded Norfloxacin With Improved Flexibility and Antibacterial Activity for Wound Dressing Application

Composite Film of Chitosan Loaded Norfloxacin With Improved Flexibility and Antibacterial Activity for Wound Dressing Application

Chitosan Silver Nano Composites (Cagncs) as Potential Antibacterial Agent to Control Vibrio tapetis

Antimicrobial Properties of a Chitosan Dextran-Based Hydrogel for Surgical Use

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