Chitosan chemically modified to deliver nitric oxide with high antibacterial activity

Pelegrino, Milena T.; Pieretti, Joana C.; Nakazato, Gerson; Gonçalves, Marcelly Chue; Moreira, José Carlos Barbosa; Seabra, Amedea B.

doi:10.1016/j.niox.2020.10.003

Cited by 31 publications

(17 citation statements)

References 53 publications

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“…2d . We have recently reported that the NO release profile from S-nitroso-chitosan (without the nanoplatform) was comprised of an initial rapid NO release for 1 h [ 37 ], whereas Fe 3 O 4 @Ag/CS-NO NPs presented an initial burst of NO release after 3 h of monitoring.…”

Section: Resultsmentioning

confidence: 99%

“…For future research, the amounts of NO, AgNPs and Fe 3 O 4 NPs contents in the final Fe 3 O 4 @Ag/CS-NO NPs can be tailored to treat different types of tumors, achieving the desired cell death pathway or decreasing cell proliferation. Moreover, Fe 3 O 4 NPs are well-known for their magnetic properties, which may be applied not only for NP targeting, but also in hyperthermia therapy, with important effects in the treatment of malignant tumors [ 37 ]. When exposed to alternating external magnetic field, Fe 3 O 4 may promote a local temperature increase from 43 to 46 °C [ 56 ].…”

Section: Resultsmentioning

confidence: 99%

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Multifunctional hybrid nanoplatform based on Fe3O4@Ag NPs for nitric oxide delivery: development, characterization, therapeutic efficacy, and hemocompatibility

Pieretti

Gonçalves

Nakazato

et al. 2021

J Mater Sci: Mater Med

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The combination of Fe3O4@Ag superparamagnetic hybrid nanoparticles and nitric oxide (NO) represents an innovative strategy for a localized NO delivery with a simultaneous antibacterial and antitumoral actions. Here, we report the design of Fe3O4@Ag hybrid nanoparticles, coated with a modified and nitrosated chitosan polymer, able to release NO in a biological medium. After their synthesis, physicochemical characterization confirmed the obtention of small NO-functionalized superparamagnetic Fe3O4@Ag NPs. Antibacterial assays demonstrated enhanced effects compared to control. Bacteriostatic effect against Gram-positive strains and bactericidal effect against E. coli were demonstrated. Moreover, NO-functionalized Fe3O4@Ag NPs demonstrated improved ability to reduce cancer cells viability and less cytotoxicity against non-tumoral cells compared to Fe3O4@Ag NPs. These effects were associated to the ability of these NPs act simultaneous as cytotoxic (necrosis inductors) and cytostatic compounds inducing S-phase cell cycle arrest. NPs also demonstrated low hemolysis ratio (<10%) at ideal work range, evidencing their potential for biomedical applications.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Multifunctional hybrid nanoplatform based on Fe3O4@Ag NPs for nitric oxide delivery: development, characterization, therapeutic efficacy, and hemocompatibility

Pieretti

Gonçalves

Nakazato

et al. 2021

J Mater Sci: Mater Med

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“…against Trypanosoma cruzi , 113 E. coli , S. aureus , Streptococcus mutans. 114 NO–metal complexes (zeolites), with activity against B. subtilis , Clostridium difficile , E. coli , P. aeruginosa , S. aureus (including MRSA). 110 NONOoates (diazeniumdiolates), e.g.…”

Section: Administering Nitric Oxide Donors and Related Compoundsmentioning

confidence: 99%

Nitric oxide for the prevention and treatment of viral, bacterial, protozoal and fungal infections

et al. 2021

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Although the antimicrobial potential of nitric oxide (NO) is widely published, it is little used clinically. NO is a key signalling molecule modulating vascular, neuronal, inflammatory and immune responses. Endogenous antimicrobial activity is largely mediated by high local NO concentrations produced by cellular inducible nitric oxide synthase, and by derivative reactive nitrogen oxide species including peroxynitrite and S-nitrosothiols. NO may be taken as dietary substrate (inorganic nitrate, L-arginine), and therapeutically as gaseous NO, and transdermal, sublingual, oral, intranasal and intravenous nitrite or nitrate. Numerous preclinical studies have demonstrated that NO has generic static and cidal activities against viruses (including β-coronaviruses such as SARS-CoV-2), bacteria, protozoa and fungi/yeasts in vitro. Therapeutic effects have been seen in animal models in vivo, and phase II trials have demonstrated that NO donors can reduce microbial infection. Nevertheless, excess NO, as occurs in septic shock, is associated with increased morbidity and mortality. In view of the dose-dependent positive and negative effects of NO, safety and efficacy trials of NO and its donors are needed for assessing their role in the prevention and treatment of infections. Trials should test dietary inorganic nitrate for pre- or post-exposure prophylaxis and gaseous NO or oral, topical or intravenous nitrite and nitrate for treatment of mild-to-severe infections, including due to SARS-CoV-2 (COVID-19). This review summarises the evidence base from in vitro, in vivo and early phase clinical studies of NO activity in viral, bacterial, protozoal and fungal infections.

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“…17,19 Thus, key factor linked to modulate the dichotomous effect of NO are the amount of NO released, the time-period and the spatial distribution of this signaling molecule in tumor tissue. 20 In addition, tumor microenvironment and heterogeneity also play an important role in the dichotomous effect of NO. 1 Thus, for proposing the use of exogenous NO to cancer cells/tissues, it is of fundamental importance to tailor the desired amounts of NO released to the target site of application.…”

Section: Introduction: the Biological Importance Of Nitric Oxidementioning

confidence: 99%

Recent progress in the toxicity of nitric oxide-releasing nanomaterials

et al. 2021

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Chitosan chemically modified to deliver nitric oxide with high antibacterial activity

Cited by 31 publications

References 53 publications

Multifunctional hybrid nanoplatform based on Fe3O4@Ag NPs for nitric oxide delivery: development, characterization, therapeutic efficacy, and hemocompatibility

Multifunctional hybrid nanoplatform based on Fe3O4@Ag NPs for nitric oxide delivery: development, characterization, therapeutic efficacy, and hemocompatibility

Nitric oxide for the prevention and treatment of viral, bacterial, protozoal and fungal infections

Recent progress in the toxicity of nitric oxide-releasing nanomaterials

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