Chitinolytic activity of phylogenetically diverse Bacillus cereus sensu lato from natural environments

Drewnowska, Justyna M.; Fiodor, Angelika; Barboza‐Corona, José E.; Święcicka, Izabela

doi:10.1016/j.syapm.2020.126075

Cited by 16 publications

(13 citation statements)

References 43 publications

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“…One explanation is that emetic B. cereus isolates typically inhabit the soil, so do not require carbohydrate hydrolases due to the lack of available carbohydrates in this environment. All isolates were able to liquefy gelatin, consistent with a soil-based lifestyle that requires enzymes such as collagenase and chitinase to degrade soil organics (Hwang and Park 2015;Drewnowska et al 2020).…”

Section: Biochemical Charactersmentioning

confidence: 56%

Phenotypic homogeneity of emetic Bacillus cereus isolates in China

Yang

Foster

et al. 2021

Lett Appl Microbiol

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Section: Biochemical Charactersmentioning

confidence: 56%

Phenotypic homogeneity of emetic Bacillus cereus isolates in China

Yang

Foster

et al. 2021

Lett Appl Microbiol

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“…This may be due to the high conserved nature of ISSR regions, which support the using of ISSR molecular markers in the genotyping and profiling of rhizobial isolates. The phylogenetic analysis provides different evolutionary history for particular genes or organisms (Krieg et al, 2010;Drewnowska et al, 2020;Huang et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Genetic Profiling and Diversity of Some Promising Efficient Rhizobial Isolates on Faba Bean Plants

Fathy

Eldomiaty

El-Fattah

et al. 2021

Zagazig Journal of Agricultural Research

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A group of twenty faba bean rhizobial isolates was collected from two Egyptian Governorates (Dakahlia and Damitta). The isolates were further morphologically and physiologically characterized to check their growth and symbiotic performance on faba bean plants. According to remarkable lab and pots tests, five rhizobial isolates (Rh 32, Rh 6-A, Rh 3-4, Rh RL3, and Rh 8-A) were selected and subjected to further biochemical and molecular characterizations. Genetic profiling of the five promising rhizobial isolates was conducted using six ISSR-primers. Amplification of bacterial genomic DNA produced a total of 37 genomic loci, 54% of them were polymorphic and 46% were monomorphic. The rate of polymorphism ranged between 25% to 80% with an average of 54%. Clustering pattern analysis of morphological and physiological data grouped the twenty rhizobial isolates in five clusters and the five selected rhizobial isolates were falling close to each other. Clustering analysis of ISSR data grouped the the five rhizobial isolates in four clusters. Analysis based on ISSR data revealed that the lowest genetic distance was 2.00 between Rh 6-A and Rh 3-4 isolates, while the highest genetic distance of 3.61 was between Rh 32 and each of Rh 6-A, Rh 3-4, and Rh RL3 isolates. The greatest similarity measurement was 0.931 between Rh 6-A and Rh 3-4 isolates; while the lowest similarity was 0.745 between Rh 32 and Rh 3-4 isolates. It can concluded that clustering pattern analysis based on molecular data could be used in facilitating the selection of rhizobial isolates that will be promising as a source of genes for biological nitrogen fixation and plant growth-promotion.

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“…10 μl of cell free supernatant was added to the 90 μl each of the three substrate solutions and incubated in the dark for 40min at 25 °C. Thereafter, the reactions were halted by adding 200 μl of Stop Solution (39). Fluorescence of released 4-methylumbelliferone (4MU) was measured (Excitation 360 nm, Emission 450 nm) in a plate reader (Synergy MX, Biotek).…”

Section: Methodsmentioning

confidence: 99%

Effects of interspecies interactions on marine community ecosystem function

Daniels

Stubbusch

Held

et al. 2022

Preprint

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Microbial communities perform key ecosystem processes collectively. One such process is the degradation of carbohydrate polymers, which are the dominant pool of organic carbon in natural environments. Carbohydrate polymers are often degraded in a stepwise manner. Individual steps are performed by different microbial species, which form trophic cascades with carbon polymers at the bottom and fully oxidised carbon at the top. It is widely believed that these trophic cascades are hierarchically organised, where organisms at each level rely on organisms at the levels below. However, whether and how the higher-level organisms can also affect processes at the lower levels is not well understood. Here we studied how carbohydrate polymer degradation mediated by secreted enzymes is affected by species at higher trophic levels, i.e., species that cannot produce the enzymes for polymer degradation but can grow in presence of the polymer degraders. We used growth and enzyme assays in combination with transcriptomics to study how chitin degradation by a number of Vibrio strains is affected by the presence of different cross-feeders that consume metabolic by-products. We found that interactions between the degraders and cross-feeders influence the rate of chitin degradation by the community. Furthermore, we show that this is a result of changes in chitinase expression by degraders. Overall, our results demonstrate that interactions between species can influence key ecosystem functions performed by individuals within microbial communities. These results challenge the perspective that trophic cascades based on metabolically coupled microbial communities are unidirectional and provide mechanistic insights into these downstream interactions.

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Chitinolytic activity of phylogenetically diverse Bacillus cereus sensu lato from natural environments

Cited by 16 publications

References 43 publications

Phenotypic homogeneity of emetic Bacillus cereus isolates in China

Phenotypic homogeneity of emetic Bacillus cereus isolates in China

Genetic Profiling and Diversity of Some Promising Efficient Rhizobial Isolates on Faba Bean Plants

Effects of interspecies interactions on marine community ecosystem function

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