Chiral Separation of Cetirizine Enantiomers by Cyclodextrin Mediated Capillary Electrophoresis

Abstract

Chiral separation cetirizine, a second generation H1 antagonist was studied by cyclodextrine (CD) mediated capillary electrophoresis. The influence on the separation of several parameters including pH and concentration of the background electrolyte (BGE), CD type and concentration, applied voltage and temperature were studied and the electrophoretic and analytic parameters were optimized. The best conditions for the chiral separation were obtained using 25mM disodium hydrogenophosphate -25mM sodium didydrogeno-phosphate (1:1) as BGE, 5mM sulfobuthyl ether-β-CD as chiral selector, a voltage of + 20kV, temperature of 20°C, injection pressure/time of 50mbar/ 1sec, UV detection at 230nm. The analytical performance of the method was evaluated. The proposed method was successfully applied to the enantioselective assay of cetirizine in pharmaceutical formulations. CE proved to be a rapid, specific, reliable and cost-effective method for the chiral separation of cetirizine enantiomers and can be useful for laboratories performing routine analysis.

Efficient determination of non-steroidal anti-inflammatory drugs by micellar electrokinetic chromatography in wastewater

“…33,34 The presence of an organic modifier influences the distribution of analytes between the aqueous phase and micelles; additionally, it can affect the charge of ionizable groups. 24,26,29 It is worth noting that the presence of methanol can change the order migration of KET and ASA (Fig. 3A and B), which could be explained by the fact that methanol reduced the partition coefficient between the micelle and the bulk solution.…”

“…MEKC separation is based on the differential partition of analytes between micelles and aqueous buffer, whereas CZE separation is based on differences in the analytes' electrophoretic mobilities. 24 In the presence of SDS in the BGE the analytes get separated due to the combination of electrophoretic mobility and differential interaction of the analyte with negatively charged SDS. 27 Therefore, the migration behavior of the solute depends on several properties, including hydrophobicity and the degree of dissociation in the solution.…”

“…3A and B), which could be explained by the fact that methanol reduced the partition coefficient between the micelle and the bulk solution. 24…”

“…The most commonly used surfactant is sodium dodecyl sulfate (SDS). 24 This surfactant has a low critical micelle concentration (8.1 mM in pure water at 25 °C) and provides good selectivity and efficiency. 25 Although MEKC was developed for the analysis of neutral compounds, it also proved to be useful in the separation of charged solutes.…”

“…27 Therefore, the migration behavior of the solute depends on several properties, including hydrophobicity and the degree of dissociation in the solution. 24,27,28 The different ionization constants of the ve NSAID analytes affect how well they separate. For example, PAC (pK a 9.5) is a zwitterion, which means that it has both acidic and basic groups.…”