Chinese medicinal herb extract inhibits PQS-mediated quorum sensing system in Pseudomonas aeruginosa

Wei, Qing; Bhasme, Pramod; Wang, Zhiguo; Wang, Li; Wang, Shiwei; Zeng, Yunfei; Wang, Yi; Zhang, Luyan; Li, Yan

doi:10.1016/j.jep.2019.112272

Cited by 26 publications

(20 citation statements)

References 46 publications

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“…Studies have found that many organisms can secrete quorum-sensing signal analogs, competitively combine with bacterial quorum-sensing signal receptors, interfere with the regulation of the quorum-sensing system, and significantly reduce the pathogenicity of bacteria. Wei et al [115] discovered a medicinal herb extract (MHE). The Pseudomonas quinolone signaling (PQS) system was completely suppressed, the rhlR/rhlI QS system was moderately suppressed, and the lasR/lasI QS system was only slightly affected, suggesting that MHE may selectively target the PQS system to inhibit bacterial toxicity.…”

Section: Inhibition Of Signal Molecule Conduction or Binding To Recepmentioning

confidence: 99%

Quorum-Sensing Regulation of Antimicrobial Resistance in Bacteria

2020

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Quorum sensing is a cell-to-cell communication system that exists widely in the microbiome and is related to cell density. The high-density colony population can generate a sufficient number of small molecule signals, activate a variety of downstream cellular processes including virulence and drug resistance mechanisms, tolerate antibiotics, and harm the host. This article gives a general introduction to the current research status of microbial quorum-sensing systems, focuses on the role of quorum-sensing systems in regulating microbial resistance mechanisms, such as drug efflux pump and microbial biofilm formation regulation, and discusses a new strategy for the treatment of drug-resistant bacteria proposed by using quorum quenching to prevent microbial resistance.

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Section: Inhibition Of Signal Molecule Conduction or Binding To Recepmentioning

confidence: 99%

Quorum-Sensing Regulation of Antimicrobial Resistance in Bacteria

2020

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“…For example, we have recently shown that one of the Chinese medicinal herb extracts (MHE) could interrupt the binding of MvfR to the pqsA promoter region, thus inhibiting the virulence factors production. Chemical analysis and molecular docking analysis showed that butyrolactone (BTL) and furfuryl alcohol (FA) within MHE could function as interference molecule(s) with the PQS signal to compete with MvfR (Wei et al, 2019a). Therefore, it would be interesting to identify the chemical components within TRQ to test for their performance and involvement in ligand-receptor binding interference.…”

Section: Discussionmentioning

confidence: 99%

Traditional Chinese Medicine Tanreqing Inhibits Quorum Sensing Systems in Pseudomonas aeruginosa

et al. 2020

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Pseudomonas aeruginosa is an opportunistic pathogen that can infect a wide variety of hosts including humans, plants, and animals. The production of virulence factors is the determinant of the infection paradigm and is under orchestrated regulation via cell-to-cell communication process called quorum sensing (QS). To disable QS circuits and prevent bacterial infections, a large battery of anti-QS agents, particularly from traditional Chinese medicine have been developed. Here, we used P. aeruginosa as a model microorganism to investigate the effect of traditional Chinese medicine Tanreqing (TRQ) formula on bacterial pathogenicity. Phenotypic analysis showed that TRQ treatment could completely inhibit the production of phenazine pyocyanin and moderately inhibit the production of virulence factors such as rhamnolipids, elastase, and alkaline protease. Further transcriptomic analyses revealed that TRQ treatment could significantly attenuate the expression of QS-regulated genes in P. aeruginosa and TRQ-treated P. aeruginosa regulon shared a large overlap with QS regulon. Component contribution to QS inhibition shed light on the indispensable role of all five components in TRQ formula. Further genetic analysis indicated that upstream regulators of QS systems, including two-component systems GacS/GacA and PprA/PprB, were both inhibited by TRQ treatment. Finally, our TRQ formula could efficiently protect Caenorhabditis elegans from killing by P. aeruginosa. Altogether, we have proved TRQ formula as an effective and specific agent to attenuate bacterial virulence and combat bacterial infections.

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“…A better understanding of QS signal molecules will provide critical information for the development of chemicals or methods for controlling biofilm. This has become a hot research area in the search for an effective molecule that can either neutralize QS molecules or compete with their receptors [41][42][43].…”

Section: Antibiotic Resistance Through Quorum Sensingmentioning

confidence: 99%

“…Two types of anti-QS chemicals are particularly promising, namely phytochemicals and plant by-products [72]. Researchers are also actively seeking natural compounds that possess anti-quorum sensing properties [43,[72][73][74]. Anti-QS agents are mechanistically sound, promising a new class of agents to tackle biofilms.…”

Section: Block Quorum Sensing Signalingmentioning

confidence: 99%

Mechanisms and Control Strategies of Antibiotic Resistance in Pathological Biofilms

Ying¹,

Yang²,

Zhang³

et al. 2021

J. Microbiol. Biotechnol.

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Biofilms are protected microbial structures enclosed by a self-secreted extracellular matrix [1]. Bacteria biofilm is one of the most successful forms of life, being widely distributed in a diversity of environments [2]. All higher organisms (including humans) are colonized by microorganisms that form biofilms, mostly bacteria [3]. The ability of biofilms to escape the host immune system and resist antibiotics poses great health threats to patients [4,5].Biofilm formation contributes to drug resistance and inflammation, causing persistent infections in patients [6]. Biofilms also function as cell reservoirs that can repopulate infection sites after the release of suppression. For example, biofilm formation is a major concern for cystic fibrosis pneumonia caused by Pseudomonas aeruginosa and urinary tract infections caused by Escherichia coli, both of which are refractory to multiple antibiotics [7]. Therefore, basic research on biofilm formation and the mechanism of their resistance is of great clinical relevance. The objective of this review is to summarize the current research progress in antibiotic resistance mechanisms associated with bacterial biofilms and anti-biofilm strategies, which may greatly benefit patients who suffer from biofilm-related infections. Bacterial Biofilms Structure and Characteristics of Bacterial BiofilmThe physical scaffold of a biofilm is the matrix of extracellular polymeric substances (EPSs), self-secreted substances that keep bacterial cells in a contained structure and attach them to surfaces [8]. Most of the biomass of the biofilm is hydrated EPS rather than bacterial cells, which only make up between 2% to 15% of the total biofilm mass [9]. EPS is mostly composed of polysaccharides, proteins, lipids and extracellular DNA (eDNA) (Fig. 1) [9]. Enhanced antimicrobial resistance, nutrient capture and social cooperation are three main characteristic features of biofilms, and the EPS matrix underlies these important properties [2]. The structures of biofilms somewhat resembles tissues of higher organisms, which are structurally complex and highly heterogenous in gene expression [10], both contributing to the resistance mechanisms of biofilm. Life Cycle of Bacterial BiofilmsThe transition from planktonic growth to biofilm is a complex and highly regulated process that follows a few steps (Fig. 1). The first step is the initial attachment of planktic bacterial cells to a surface, which often happens when cells are under environmental stress. The propelling structures, such as appendages, fimbriae and sex pili, Bacterial biofilm is a community of bacteria that are embedded and structured in a self-secreted extracellular matrix. An important clinical-related characteristic of bacterial biofilms is that they are much more resistant to antimicrobial agents than the planktonic cells (up to 1,000 times), which is one of the main causes of antibiotic resistance in clinics. Therefore, infections caused by biofilms are notoriously difficult to eradicate, such as lung infection caused by Pseudom...

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Chinese medicinal herb extract inhibits PQS-mediated quorum sensing system in Pseudomonas aeruginosa

Cited by 26 publications

References 46 publications

Quorum-Sensing Regulation of Antimicrobial Resistance in Bacteria

Quorum-Sensing Regulation of Antimicrobial Resistance in Bacteria

Traditional Chinese Medicine Tanreqing Inhibits Quorum Sensing Systems in Pseudomonas aeruginosa

Mechanisms and Control Strategies of Antibiotic Resistance in Pathological Biofilms

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